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ENOX2 Polyclonal Antibody

ENOX2 Antibody in Immunocytochemistry (ICC/IF)

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ENOX2 Antibody (10423-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 10423-1-AP (ENOX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ENOX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ENOX2 Antibody in Immunoprecipitation (IP)

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Product Details

10423-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ENOX2 Fusion Protein Ag0674 (292-610 aa encoded by BC019254)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MIQSANSHV RRLVNEKAAH EKDMEEAKEK FKQALSGILI QFEQIVAVYH SASKQKAWDH FTKAQRKNIS VWCKQAEEIR NIHNDELMGI RREEEMEMSD DEIEEMTETK ETEESVSQAE ALKEENDSLR WQLDAYRNEV ELLKQEQGKV HREDDPNKEQ QLKLLQQALQ GMQQHLLKVQ EEYKKKEAEL EKLKDDKLQV EKMLENLKEK ESCASRLCAS NQDSEYPLEK TMNSSPIKSE REALLVGIIS TFLHVHPFGA SIEYICSYLH RLDNKICTSD VECLMGRLQH TFKQEMTGVG ASLEKRWKFC GFEGLKLT (292-610 aa encoded by BC019254)

Target Information

This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bis(chloroacetamidino)-Derived Heteroarene-Fused Anthraquinones Bind to and Cause Proteasomal Degradation of tNOX, Leading to c-Flip Downregulation and Apoptosis in Oral Cancer Cells.

Cancers

Chang JS,Chen CY,Tikhomirov AS,Islam A,Liang RH,Weng CW,Wu WH,Shchekotikhin AE,Chueh PJ

Wed Sep 28 00:00:00 UTC 2022

Capsaicin exerts therapeutic effects by targeting tNOX-SIRT1 axis and augmenting ROS-dependent autophagy in melanoma cancer cells.

American journal of cancer research

Islam A,Hsieh PF,Liu PF,Chou JC,Liao JW,Hsieh MK,Chueh PJ

Fri Oct 22 00:00:00 UTC 2021

Capsaicin acts through tNOX (ENOX2) to induce autophagic apoptosis in p53-mutated HSC-3 cells but autophagy in p53-functional SAS oral cancer cells.

American journal of cancer research

Chang CF,Islam A,Liu PF,Zhan JH,Chueh PJ

Thu Nov 12 00:00:00 UTC 2020

Synthesis and Characterization of 4,11-Diaminoanthra[2,3-b]furan-5,10-diones: Tumor Cell Apoptosis through tNOX-Modulated NAD(+)/NADH Ratio and SIRT1.

Journal of medicinal chemistry

Tikhomirov AS,Shchekotikhin AE,Lee YH,Chen YA,Yeh CA,Tatarskiy VV,Dezhenkova LG,Glazunova VA,Balzarini J,Shtil AA,Preobrazhenskaya MN,Chueh PJ

Thu Dec 24 00:00:00 UTC 2015

Synthesis and Characterization of 4,11-Diaminoanthra[2,3-b]furan-5,10-diones: Tumor Cell Apoptosis through tNOX-Modulated NAD(+)/NADH Ratio and SIRT1.

Journal of medicinal chemistry

Tikhomirov AS,Shchekotikhin AE,Lee YH,Chen YA,Yeh CA,Tatarskiy VV,Dezhenkova LG,Glazunova VA,Balzarini J,Shtil AA,Preobrazhenskaya MN,Chueh PJ

Thu Dec 24 00:00:00 UTC 2015

Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo.

Journal of agricultural and food chemistry

Liu NC,Hsieh PF,Hsieh MK,Zeng ZM,Cheng HL,Liao JW,Chueh PJ

Wed Mar 14 00:00:00 UTC 2012

Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo.

Journal of agricultural and food chemistry

Liu NC,Hsieh PF,Hsieh MK,Zeng ZM,Cheng HL,Liao JW,Chueh PJ

Wed Mar 14 00:00:00 UTC 2012

Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells.

Experimental cell research

Zeng ZM,Chuang SM,Chang TC,Hong CW,Chou JC,Yang JJ,Chueh PJ

Wed Aug 15 00:00:00 UTC 2012

Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells.

Experimental cell research

Zeng ZM,Chuang SM,Chang TC,Hong CW,Chou JC,Yang JJ,Chueh PJ

Wed Aug 15 00:00:00 UTC 2012

Chemotherapeutic agents enhance cell migration and epithelial-to-mesenchymal transition through transient up-regulation of tNOX (ENOX2) protein.

Biochimica et biophysica acta

Su YC,Lin YH,Zeng ZM,Shao KN,Chueh PJ

Thu Nov 01 00:00:00 UTC 2012

Chemotherapeutic agents enhance cell migration and epithelial-to-mesenchymal transition through transient up-regulation of tNOX (ENOX2) protein.

Biochimica et biophysica acta

Su YC,Lin YH,Zeng ZM,Shao KN,Chueh PJ

Thu Nov 01 00:00:00 UTC 2012

Down-regulation of tumor-associated NADH oxidase, tNOX (ENOX2), enhances capsaicin-induced inhibition of gastric cancer cell growth.

Cell biochemistry and biophysics

Wang HM,Chuang SM,Su YC,Li YH,Chueh PJ

Tue Nov 01 00:00:00 UTC 2011

Down-regulation of tumor-associated NADH oxidase, tNOX (ENOX2), enhances capsaicin-induced inhibition of gastric cancer cell growth.

Cell biochemistry and biophysics

Wang HM,Chuang SM,Su YC,Li YH,Chueh PJ

Tue Nov 01 00:00:00 UTC 2011

RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac.

Biochemical and biophysical research communications

Liu SC,Yang JJ,Shao KN,Chueh PJ

Fri Jan 25 00:00:00 UTC 2008

RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac.

Biochemical and biophysical research communications

Liu SC,Yang JJ,Shao KN,Chueh PJ

Fri Jan 25 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: APK1 antigen; Cytosolic ovarian carcinoma antigen 1; Ecto-NOX disulfide-thiol exchanger 2; tNOX; Tumor-associated hydroquinone oxidase

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Gene Aliases: APK1; COVA1; ENOX2; tNOX

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UniProt ID: (Human) Q16206, (Mouse) Q8R0Z2

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Entrez Gene ID: (Human) 10495, (Mouse) 209224

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Function(s)

nucleotide binding nucleic acid binding protein disulfide oxidoreductase activity N-ethylmaleimide reductase activity oxidoreductase activity reduced coenzyme F420 dehydrogenase activity sulfur oxygenase reductase activity malolactic enzyme activity NADPH:sulfur oxidoreductase activity epoxyqueuosine reductase activity

Process(es)

transport ultradian rhythm regulation of growth rhythmic process oxidation-reduction process cell growth

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ENOX2 Polyclonal Antibody

ENOX2 Antibody (10423-1-AP) in ICC/IF

Product Details

10423-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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