Invitrogen

ENPP2 Polyclonal Antibody

2 Published Figures

4 References

View all (21) ENPP2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (2)

ENPP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

ENPP2 Antibody (PA5-12478) in IHC (P)

Immunohistochemistry analysis of ENPP2 in formalin fixed and paraffin embedded human hepatocarcinoma. Samples were incubated with ENPP2 polyclonal antibody (Product # PA5-12478) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ENPP2 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-12478

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Flow Cytometry (Flow)

1:10-1:50

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 401-430 amino acids from the central region of human ENPP2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Size-exclusion - Dialysis, Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2231274

Product Specific Information

This antibody is predicted to react with bovine based on sequence homology.

Target Information

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endothelial ENPP2 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 2) Increases Atherosclerosis in Female and Male Mice.

Arteriosclerosis, thrombosis, and vascular biology

Karshovska E,Mohibullah R,Zhu M,Zahedi F,Thomas D,Magkrioti C,Geissler C,Megens RTA,Bianchini M,Nazari-Jahantigh M,Ferreirós N,Aidinis V,Schober A

PA5-12478 was used in Immunohistochemistry to demonstrate that the expression of ENPP2 in ECs promotes atherosclerosis and endothelial inflammation in a sex-independent manner.

Mon Aug 01 00:00:00 UTC 2022

Comparative Proteomic Profiling Identifies Reciprocal Expression of Mitochondrial Proteins Between White and Gray Matter Lesions From Multiple Sclerosis Brains.

Frontiers in neurology

Rai NK,Singh V,Li L,Willard B,Tripathi A,Dutta R

PA5-12478 was used in Immunohistochemistry to provide evidence of opposite expression of mitochondrial proteins in response to demyelination of white- and gray-matter regions in progressive MS brain.

Sat Jul 16 00:00:00 UTC 2022

Comparative Proteomic Profiling Identifies Reciprocal Expression of Mitochondrial Proteins Between White and Gray Matter Lesions From Multiple Sclerosis Brains.

Frontiers in neurology

Rai NK,Singh V,Li L,Willard B,Tripathi A,Dutta R

PA5-12478 was used in Immunohistochemistry to provide evidence of opposite expression of mitochondrial proteins in response to demyelination of white- and gray-matter regions in progressive MS brain.

Sat Jul 16 00:00:00 UTC 2022

Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease.

British journal of pharmacology

Sikura KÉ,Potor L,Szerafin T,Oros M,Nagy P,Méhes G,Hendrik Z,Zarjou A,Agarwal A,Posta N,Torregrossa R,Whiteman M,Fürtös I,Balla G,Balla J

PA5-12478 was used in Western Blot to investigate H2 S as a potential therapeutic in valvular calcification and to identify its targets in the pathogenesis.

Sat Feb 01 00:00:00 UTC 2020

Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease.

British journal of pharmacology

Sikura KÉ,Potor L,Szerafin T,Oros M,Nagy P,Méhes G,Hendrik Z,Zarjou A,Agarwal A,Posta N,Torregrossa R,Whiteman M,Fürtös I,Balla G,Balla J

PA5-12478 was used in Western Blot to investigate H2 S as a potential therapeutic in valvular calcification and to identify its targets in the pathogenesis.

Sat Feb 01 00:00:00 UTC 2020

Interaction of Autotaxin With Lipoprotein(a) in Patients With Calcific Aortic Valve Stenosis.

JACC. Basic to translational science

Bourgeois R,Devillers R,Perrot N,Després AA,Boulanger MC,Mitchell PL,Guertin J,Couture P,Boffa MB,Scipione CA,Pibarot P,Koschinsky ML,Mathieu P,Arsenault BJ

PA5-12478 was used in Absorbance assay to determine whether autotaxin (ATX) is transported by lipoprotein(a) [Lp(a)] in human plasma and if could be used as a biomarker of calcific aortic valve stenosis (CAVS).

Tue Sep 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Autotaxin; autotaxin-t; AutotaxinATX; E-NPP 2; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; Extracellular lysophospholipase D; FLJ26803; LysoPLD; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D

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Gene Aliases: ATX; ATX-X; AUTOTAXIN; ENPP2; LysoPLD; NPP2; PD-IALPHA; PDNP2

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UniProt ID: (Human) Q13822

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Entrez Gene ID: (Human) 5168

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Function(s)

nucleic acid binding phosphodiesterase I activity nucleotide diphosphatase activity lysophospholipase activity scavenger receptor activity calcium ion binding transcription factor binding zinc ion binding hydrolase activity polysaccharide binding alkylglycerophosphoethanolamine phosphodiesterase activity

Process(es)

phosphate-containing compound metabolic process receptor-mediated endocytosis movement of cell or subcellular component chemotaxis immune response G-protein coupled receptor signaling pathway phospholipid catabolic process positive regulation of epithelial cell migration regulation of cell migration phosphatidylcholine catabolic process regulation of angiogenesis positive regulation of peptidyl-tyrosine phosphorylation nucleic acid phosphodiester bond hydrolysis positive regulation of lamellipodium morphogenesis

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ENPP2 Polyclonal Antibody

ENPP2 Antibody (PA5-12478) in IHC (P)

Product Details

PA5-12478

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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