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ENY2 Antibody (PA5-34959) in WB
Western Blot using ENY2 Polyclonal Antibody (Product # PA5-34959). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 Polyclonal Antibody (Product # PA5-34959) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-34959
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ENY2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2552308
Product Specific Information
Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (86%), Xenopus laevis (93%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ENY2 is a component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. May also participate in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: enhancer of yellow 2 homolog; Enhancer of yellow 2 transcription factor homolog; ENY 2; ENY-2; ENY2; Transcription and mRNA export factor ENY2
Gene Aliases: DC6; e(y)2; ENY2; Sus1
UniProt ID: (Human) Q9NPA8
Entrez Gene ID: (Human) 56943
regulation of transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
protein transport
histone deubiquitination
poly(A)+ mRNA export from nucleus
positive regulation of transcription, DNA-templated
negative regulation of insulin secretion involved in cellular response to glucose stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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