Invitrogen

EOMES Monoclonal Antibody (21Mags8), eBioscience™

FIGURE: 1 / 4

EOMES Antibody (14-4876-82) in WB

Mouse spleen cell lysate were resolved by SDS-PAGE and electroblotted onto a PVDF membrane. The membrane was probed with 2 µg/mL of Anti-Human/Mouse EOMES Purified and revealed using an Anti-Rat IgG HRP antibody.

Product Image Gallery

EOMES Antibody — Figure 5 T-bet-deficient T cells are hyper-responsive to IL-23. C57BL/6 Rag1 -/- mice were injected i.p. with either WT or Tbx21 -/- CD4 + CD25 - CD45RB hi T cells or a 1:1 mixtures of WT (CD45.1 + ) and Tbx21 -/- (CD45.2 + ) CD4 + CD25 - CD45RB hi T cells. Mice were killed when recipients of Tbx21 -/- T cells developed clinical signs of disease (~5 weeks) and assessed for intestinal inflammation. ( a ) Colitis scores. ( b ) Frequencies of IL-17A + and/or IFN-gamma + cells in the colon of mice receiving mix of WT and Tbx21 -/- CD4 + T cells. ( c ) CD4 + T cells were purified by flow cytometry from the inflamed colon of mice receiving a mix of WT and Tbx21 -/- CD4 + T cells and mRNA levels of indicated genes were analysed by quantitative reverse transcription PCR (qRT-PCR). ( d ) ChIP-qRT-PCR for the occupancy of H3K4me3 or RNA Pol II around the TSS of indicated genes in CD4 + T cells purified as described in c . ( e ) Immunoblot for pY705-STAT3, total STAT3, EOMES, Runx3 and Actin on whole cells lysates of CD4 + T cells purified as in c , left unstimulated or stimulated with IL-23 for 12 h. ( f ) Concentrations of indicated cytokines secreted into culture medium by CD4 + T cells purified and stimulated as described in e . Data in a - c and f represent results from one of two independent experiments ( n =5 for WT, n =6 for Tbx21 -/- , n =6 for WT+ Tbx21 -/- ). Bars are the mean and each symbol represents an individual mouse. Bars are the mean and error bars represent s.e.m. Da {TM}

Product Details

14-4876-82

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

21Mags8

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1210517

Product Specific Information

Description: This 21Mags8 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Note: For detection of mouse Eomes, we recommend 12-4875 and 51-4875 and for detection of human Eomes, we recommend 51-4877 due to the brighter staining intensity of these products compared to clone 21Mags8.

Applications Reported: This 21Mags8 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).

Applications Tested: This 21Mags8 antibody has been tested by western blot analysis of mouse splenocytes. As a starting concentration, this antibody can be used at 2 µg/mL.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

T-bet is a key modulator of IL-23-driven pathogenic CD4(+) T cell responses in the intestine.

Nature communications

Krausgruber T,Schiering C,Adelmann K,Harrison OJ,Chomka A,Pearson C,Ahern PP,Shale M,Oukka M,Powrie F

14-4876 was used in Western Blotting to identify T-bet as a key modulator of IL-23-driven colitogenic responses in the intestine.

Thu May 19 00:00:00 UTC 2016

TBX3 Directs Cell-Fate Decision toward Mesendoderm.

Stem cell reports

Weidgang CE,Russell R,Tata PR,Kühl SJ,Illing A,Müller M,Lin Q,Brunner C,Boeckers TM,Bauer K,Kartikasari AE,Guo Y,Radenz M,Bernemann C,Weiß M,Seufferlein T,Zenke M,Iacovino M,Kyba M,Schöler HR,Kühl M,Liebau S,Kleger A

14487682 was used in immunocytochemistry to report that TBX3 is an upstream regulator of the mesendoderm transcriptional program during gastrulation

Mon May 11 00:00:00 UTC 2015

Unique features of naive CD8+ T cell activation by IL-2.

Journal of immunology (Baltimore, Md. : 1950)

Cho JH,Kim HO,Kim KS,Yang DH,Surh CD,Sprent J

14-4876 was used in Western Blotting to demonstrate that IL-2 elicits a unique signaling pattern associated with strong lymphocyte-specific protein tyrosine kinase/JAK3-dependent activation of the PI3K/AKT pathway.

Sun Dec 01 00:00:00 UTC 2013

The T-box transcription factor Eomesodermin acts upstream of Mesp1 to specify cardiac mesoderm during mouse gastrulation.

Nature cell biology

Costello I,Pimeisl IM,Dräger S,Bikoff EK,Robertson EJ,Arnold SJ

14-4876 was used in Immunofluorescence to show that Eomes governs transcriptional programmes that sequentially specify cardiac and definitive endoderm progenitors during gastrulation.

Sun Aug 07 00:00:00 UTC 2011

The T-box transcription factor Eomesodermin acts upstream of Mesp1 to specify cardiac mesoderm during mouse gastrulation.

Nature cell biology

Costello I,Pimeisl IM,Dräger S,Bikoff EK,Robertson EJ,Arnold SJ

14-4876 was used in Immunofluorescence to show that Eomes governs transcriptional programmes that sequentially specify cardiac and definitive endoderm progenitors during gastrulation.

Sun Aug 07 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

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Gene Aliases: C77258; EOMES; TBR-2; TBR2

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UniProt ID: (Human) O95936, (Mouse) O54839

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Entrez Gene ID: (Human) 8320, (Mouse) 13813

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification trophectodermal cell differentiation adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response transcription, DNA-templated brain development cardioblast differentiation stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation blastocyst development immune system process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development gastrulation regulation of gene expression cell differentiation regulation of neurogenesis

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EOMES Monoclonal Antibody (21Mags8), eBioscience™

EOMES Antibody (14-4876-82) in WB

Product Details

14-4876-82

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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