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FIGURE: 1 / 1
EPB42 Antibody (PA5-109686) in WB
Western blot analysis of EPB42 in various lysates. Samples were incubated with EPB42 Polyclonal antibody (Product # PA5-109686) using a dilution of 1:800, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
Product Details
PA5-109686
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 10-240 of human EPB42 (NP_001107606).
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855097
Product Specific Information
Immunogen sequence: CDFQAARNNE EHHTKALSSR RLFVRRGQPF TIILYFRAPV RAFLPALKKV ALTAQTGEQP SKINRTQATF PISSLGDRKW WSAVVEERDA QSWTISVTTP ADAVIGHYSL LLQVSGRKQL LLGQFTLLFN PWNREDAVFL KNEAQRMEYL LNQNGLIYLG TADCIQAESW DFGQFEGDVI DLSLRLLSKD KQVEKWSQPV HVARVLGALL HFLKEQRVLP TPQTQATQEG A
Target Information
Erythrocyte membrane protein band 4.2 is an ATP-binding protein which may regulate the association of protein 3 with ankyrin. It probably has a role in erythrocyte shape and mechanical property regulation. Mutations in the EPB42 gene are associated with recessive spherocytic elliptocytosis and recessively transmitted hereditary hemolytic anemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Erythrocyte membrane protein band 4.2; Erythrocyte protein 4.2; erythrocyte surface protein band 4.2; P4.2; Protein 4.2
Gene Aliases: E42P; Epb4.2; EPB42; PA; SPH5
UniProt ID: (Human) P16452, (Mouse) P49222
Entrez Gene ID: (Human) 2038, (Mouse) 13828
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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