Proteintech

EPHB4 Polyclonal Antibody

EPHB4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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EPHB4 Antibody (20883-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue slide using (Product # 20883-1-AP) EPHB4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

20883-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EPHB4 Fusion Protein Ag10033 (639-987 aa encoded by BC052804)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KKESCVAIK TLKGGYTERQ RREFLSEASI MGQFEHPNII RLEGVVTNSM PVMILTEFME NGALDSFLRL NDGQFTVIQL VGMLRGIASG MRYLAEMSYV HRDLAARNIL VNSNLVCKVS DFGLSRFLEE NSSDPTYTSS LGGKIPIRWT APEAIAFRKF TSASDAWSYG IVMWEVMSFG ERPYWDMSNQ DVINAIEQDY RLPPPPDCPT SLHQLMLDCW QKDRNARPRF PQVVSALDKM IRNPASLKIV ARENGGASHP LLDQRQPHYS AFGSVGEWLR AIKMGRYEES FAAAGFGSFE LVSQISAEDL LRIGVTLAGH QKKILASVQH MKSQAKPGTP GGTGGPAPQY (639-987 aa encoded by BC052804)

Target Information

EPHB4 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The role of ephrinB2-EphB4 signalling in bone remodelling during orthodontic tooth movement.

Orthodontics & craniofacial research

Jiang Y,Tao G,Guan Y,Chen S,He Y,Li T,Zou S,Li Y

Wed Feb 01 00:00:00 UTC 2023

Extracellular signal-regulated kinase inhibition prevents venous adaptive remodeling via regulation of Eph-B4.

Vascular

Guo Y,Zhu F,Zhang X,Wu G,Fu P,Yang J

Tue Feb 01 00:00:00 UTC 2022

Extracellular signal-regulated kinase inhibition prevents venous adaptive remodeling via regulation of Eph-B4.

Vascular

Guo Y,Zhu F,Zhang X,Wu G,Fu P,Yang J

Tue Feb 01 00:00:00 UTC 2022

Extracellular signal-regulated kinase inhibition prevents venous adaptive remodeling via regulation of Eph-B4.

Vascular

Guo Y,Zhu F,Zhang X,Wu G,Fu P,Yang J

Tue Feb 01 00:00:00 UTC 2022

Biodegraded PCl and gelatin fabricated vascular patch in rat aortic and inferior vena cava angioplasty.

Microvascular research

Bai H,Xie B,Li M,Sun P,Wei S,Zhang L,Zhang C

Sun May 01 00:00:00 UTC 2022

Wood-Derived Vascular Patches Loaded With Rapamycin Inhibit Neointimal Hyperplasia.

Frontiers in bioengineering and biotechnology

Xie B,Zhang L,Lou C,Wei S,Li J,Bai H,Dardik A

Sat Aug 06 00:00:00 UTC 2022

Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1α pathways in breast cancer.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Su Q,Wang J,Wu Q,Ullah A,Ghauri MA,Sarwar A,Chen L,Liu F,Zhang Y

Thu Apr 01 00:00:00 UTC 2021

Bach1 plays an important role in angiogenesis through regulation of oxidative stress.

Microvascular research

Yusoff FM,Maruhashi T,Kawano KI,Nakashima A,Chayama K,Tashiro S,Igarashi K,Higashi Y

Mon Mar 01 00:00:00 UTC 2021

The application of tissue-engineered fish swim bladder vascular graft.

Communications biology

Bai H,Sun P,Wu H,Wei S,Xie B,Wang W,Hou Y,Li J,Dardik A,Li Z

Tue Oct 05 00:00:00 UTC 2021

Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling.

Journal of cellular physiology

Li T,Wang H,Lv C,Huang L,Zhang C,Zhou C,Zou S,Duan P

Mon Mar 01 00:00:00 UTC 2021

Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling.

Journal of cellular physiology

Li T,Wang H,Lv C,Huang L,Zhang C,Zhou C,Zou S,Duan P

Mon Mar 01 00:00:00 UTC 2021

The role of EphB4/ephrinB2 signaling in root repair after orthodontically-induced root resorption.

American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics

Li T,Wang H,Liu R,Wang X,Huang L,Wu Z,Yin X,Zou S,Duan P

Mon Mar 01 00:00:00 UTC 2021

Adult Human Vein Grafts Retain Plasticity of Vessel Identity.

Annals of vascular surgery

Bai H,Wang Z,Li M,Sun P,Wei S,Wang Z,Xing Y,Dardik A

Thu Oct 01 00:00:00 UTC 2020

Kinome multigenic panel identified novel druggable EPHB4-V871I somatic variant in high-risk neuroblastoma.

Journal of cellular and molecular medicine

Andolfo I,Lasorsa VA,Manna F,Rosato BE,Formicola D,Iolascon A,Capasso M

Mon Jun 01 00:00:00 UTC 2020

Immunohistochemical assessment of Eph/ephrin expression in oral squamous cell carcinoma and precursor lesions.

Odontology

Saito H,Oikawa M,Kouketsu A,Takahashi T,Kumamoto H

Wed Apr 01 00:00:00 UTC 2020

Low-level blast exposure disrupts gliovascular and neurovascular connections and induces a chronic vascular pathology in rat brain.

Acta neuropathologica communications

Gama Sosa MA,De Gasperi R,Perez Garcia GS,Perez GM,Searcy C,Vargas D,Spencer A,Janssen PL,Tschiffely AE,McCarron RM,Ache B,Manoharan R,Janssen WG,Tappan SJ,Hanson RW,Gandy S,Hof PR,Ahlers ST,Elder GA

Wed Jan 09 00:00:00 UTC 2019

Low-level blast exposure disrupts gliovascular and neurovascular connections and induces a chronic vascular pathology in rat brain.

Acta neuropathologica communications

Gama Sosa MA,De Gasperi R,Perez Garcia GS,Perez GM,Searcy C,Vargas D,Spencer A,Janssen PL,Tschiffely AE,McCarron RM,Ache B,Manoharan R,Janssen WG,Tappan SJ,Hanson RW,Gandy S,Hof PR,Ahlers ST,Elder GA

Wed Jan 09 00:00:00 UTC 2019

Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD.

Frontiers in pharmacology

Grandi A,Zini I,Palese S,Giorgio C,Tognolini M,Marchesani F,Bruno S,Flammini L,Cantoni AM,Castelli R,Lodola A,Fusari A,Barocelli E,Bertoni S

Wed Sep 30 00:00:00 UTC 2020

Tumor necrosis factor-alpha inhibits osteogenic differentiation of pre-osteoblasts by downregulation of EphB4 signaling via activated nuclear factor-kappaB signaling pathway.

Journal of periodontal research

Wang LM,Zhao N,Zhang J,Sun QF,Yang CZ,Yang PS

Thu Feb 01 00:00:00 UTC 2018

Tumor necrosis factor-alpha inhibits osteogenic differentiation of pre-osteoblasts by downregulation of EphB4 signaling via activated nuclear factor-kappaB signaling pathway.

Journal of periodontal research

Wang LM,Zhao N,Zhang J,Sun QF,Yang CZ,Yang PS

Thu Feb 01 00:00:00 UTC 2018

Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres.

Small (Weinheim an der Bergstrasse, Germany)

Guo X,Li W,Zhou J,Hou W,Wen X,Zhang H,Kong F,Luo L,Li Q,Du Y,You J

Sat Apr 01 00:00:00 UTC 2017

Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres.

Small (Weinheim an der Bergstrasse, Germany)

Guo X,Li W,Zhou J,Hou W,Wen X,Zhang H,Kong F,Luo L,Li Q,Du Y,You J

Sat Apr 01 00:00:00 UTC 2017

Low concentrations of TNF-α promote osteogenic differentiation via activation of the ephrinB2-EphB4 signalling pathway.

Cell proliferation

Wang L,Zhang J,Wang C,Qi Y,Du M,Liu W,Yang C,Yang P

Wed Feb 01 00:00:00 UTC 2017

Low concentrations of TNF-α promote osteogenic differentiation via activation of the ephrinB2-EphB4 signalling pathway.

Cell proliferation

Wang L,Zhang J,Wang C,Qi Y,Du M,Liu W,Yang C,Yang P

Wed Feb 01 00:00:00 UTC 2017

Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging.

Drug delivery

Wang Z,Xuan S,Qiu W,Zhu J,Guo X,Li W,Zhang H,Zhu X,Du YZ,You J

Wed Nov 01 00:00:00 UTC 2017

Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging.

Drug delivery

Wang Z,Xuan S,Qiu W,Zhu J,Guo X,Li W,Zhang H,Zhu X,Du YZ,You J

Wed Nov 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Developmental kinase 2; EPH receptor B4; ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; mDK-2; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyrosine kinase MYK-1; tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11

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Gene Aliases: AI042935; EPHB4; HTK; MDK2; MYK1; TYRO11

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UniProt ID: (Human) P54760, (Mouse) P54761

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Entrez Gene ID: (Human) 2050, (Rat) 686310, (Mouse) 13846

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Function(s)

transmembrane receptor protein tyrosine kinase activity ephrin receptor activity protein binding ATP binding nucleotide binding protein kinase activity protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

angiogenesis cell migration involved in sprouting angiogenesis heart morphogenesis cell adhesion peptidyl-tyrosine phosphorylation protein autophosphorylation ephrin receptor signaling pathway protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EPHB4 Polyclonal Antibody

EPHB4 Antibody (20883-1-AP) in IHC (P)

Product Details

20883-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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