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Invitrogen
EPS8 Polyclonal Antibody
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FIGURE: 1 / 6
EPS8 Antibody (PA5-18196) in ICC/IF
Immunofluorescence analysis of EPS8 was performed using 70% confluent log phase RAW 264.7 cells treated with LPS (100 ng/mL, 24h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EPS8 Polyclonal Antibody (Product # PA5-18196) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor®... View More
Product Details
PA5-18196
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (SGVESFDEGSSH) corresponding to the C-terminus amino acids of EPS8 (aa 811-822).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10988198
Product Specific Information
This antibody is predicted to react with canine, human, mouse, porcine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.
Target Information
This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Epidermal growth factor receptor kinase substrate 8
Gene Aliases: AW261790; DFNB102; EPS8
UniProt ID: (Human) Q12929, (Mouse) Q08509
Entrez Gene ID: (Human) 2059, (Mouse) 13860
adult locomotory behavior
regulation of cell shape
exit from mitosis
Rac protein signal transduction
regulation of actin filament length
actin cytoskeleton reorganization
dendritic cell migration
behavioral response to ethanol
barbed-end actin filament capping
actin filament bundle assembly
actin crosslink formation
actin polymerization-dependent cell motility
signal transduction
epidermal growth factor receptor signaling pathway
cell proliferation
positive regulation of signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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