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ERAP1 Antibody (TA812892) in WB
Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Cat # PS100001, Left lane) or pCMV6-ENTRY ERAP1 (Cat # RC205249, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and blotted with anti-ERAP1 (Cat # TA812892)(1:250).
Product Details
TA812892
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI4H2
Immunogen
Human recombinant protein fragment corresponding to amino acids 529-941 of human ERAP1 (NP_001035548) produced in E.coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 50% glycerol, 1% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is a 120 kDa protein localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. Peptides that are presented by MHC class I on the surface of a cell must be 8-11 residues long, and ERAP1 specifically trims peptides of 9 amino acids or more. ERAP1 is also induced by interferon- gamma. The gene encoding human ERAP1 maps to chromosome 5q15. ERAP1 has previously been characterized as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). A-LAP is thought to inactivate several bioactive peptides, including angiotensin II and, subsequently, may be involved in the regulation of blood pressure. PILS-AP is described as playing a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and ARTS-1 is characterized as a TNFR1 binding protein that promotes TNFR1 shedding. Further research will be necessary to fully elucidate the functions of this protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-LAP; Adipocyte-derived leucine aminopeptidase; Aminopeptidase PILS; aminopeptidase regulator of TNFR1 shedding; ARTS-1; CTD-2260A17.2; Endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase 1 delta-Exon-11 isoform; endoplasmic reticulum aminopeptidase 1 delta-Exon-13 isoform; endoplasmic reticulum aminopeptidase 1 delta-Exon-14 isoform; endoplasmic reticulum aminopeptidase 1 delta-Exon-15 isoform; endoplasmic reticulum aminopeptidase associated with antigen processing; ER-aminopeptidase 1; leucyl-specific aminopeptidase PILS; PILS-AP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; VEGF-induced aminopeptidase
Gene Aliases: A-LAP; ALAP; APPILS; ARTS-1; ARTS1; ERAAP; ERAAP1; ERAP1; KIAA0525; PILS-AP; PILSA; PILSAP; UNQ584/PRO1154
UniProt ID: (Human) Q9NZ08, (Mouse) Q9EQH2
Entrez Gene ID: (Human) 51752, (Rat) 80897, (Mouse) 80898
endopeptidase activity
aminopeptidase activity
interleukin-6 receptor binding
interleukin-1, Type II receptor binding
tumor necrosis factor receptor binding
protein binding
metalloexopeptidase activity
zinc ion binding
peptide binding
metalloaminopeptidase activity
peptidase activity
metallopeptidase activity
hydrolase activity
metal ion binding
angiogenesis
adaptive immune response
antigen processing and presentation of peptide antigen via MHC class I
membrane protein ectodomain proteolysis
regulation of blood pressure
response to bacterium
antigen processing and presentation of endogenous peptide antigen via MHC class I
peptide catabolic process
regulation of innate immune response
fat cell differentiation
positive regulation of angiogenesis
proteolysis
antigen processing and presentation
immune system process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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