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ERCC1 Polyclonal Antibody

ERCC1 Antibody in Immunocytochemistry (ICC/IF)

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ERCC1 Antibody (14586-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed MCF-7 cells using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ERCC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ERCC1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14586-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ERCC1 Fusion Protein Ag6112 (1-323 aa encoded by BC052813)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDPGKDKEG VPQPSGPPAR KKFVIPLDED EVPPGVAKPL FRSTQSLPTV DTSAQAAPQT YAEYAISQPL EGAGATCPTG SEPLAGETPN QALKPGAKSN SIIVSPRQRG NPVLKFVRNV PWEFGDVIPD YVLGQSTCAL FLSLRYHNLH PDYIHGRLQS LGKNFALRVL LVQVDVKDPQ QALKELAKMC ILADCTLILA WSPEEAGRYL ETYKAYEQKP ADLLMEKLEQ DFVSRVTECL TTVKSVNKTD SQTLLTTFGS LEQLIAASRE DLALCPGLGP QKVRALGKNP RSWGKERAPN KHNLRPQSFK VKKEPKTRHS GFRL (1-323 aa encoded by BC052813)

Target Information

ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. It is responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). It participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. (Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Synergistic effects of olaparib combined with ERCC1 on the sensitivity of cisplatin in non-small cell lung cancer.

Oncology letters

Xie K,Ni X,Lv S,Zhou G,He H

Sat May 01 00:00:00 UTC 2021

Mechanistic study of lncRNA UCA1 promoting growth and cisplatin resistance in lung adenocarcinoma.

Cancer cell international

Fu J,Pan J,Yang X,Zhang Y,Shao F,Chen J,Huang K,Wang Y

Mon Sep 20 00:00:00 UTC 2021

Sulforaphane enhances the cisplatin sensitivity through regulating DNA repair and accumulation of intracellular cisplatin in ovarian cancer cells.

Experimental cell research

Gong TT,Liu XD,Zhan ZP,Wu QJ

Sat Aug 15 00:00:00 UTC 2020

Severe ineffective erythropoiesis discriminates prognosis in myelodysplastic syndromes: analysis based on 776 patients from a single centre.

Blood cancer journal

Huang H,Xu C,Gao J,Li B,Qin T,Xu Z,Ren S,Zhang Y,Jiao M,Qu S,Pan L,Hu N,Liu J,Cai W,Zhang Y,Wu D,Zhang P,Gale RP,Huang G,Zhou J,Shi L,Xiao Z

Fri Aug 14 00:00:00 UTC 2020

The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1.

Oncology research

Wei W,Ma XD,Jiang GM,Shi B,Zhong W,Sun CL,Zhao L,Hou YJ,Wang H

Tue Sep 01 00:00:00 UTC 2020

The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1.

Oncology research

Wei W,Ma XD,Jiang GM,Shi B,Zhong W,Sun CL,Zhao L,Hou YJ,Wang H

Tue Sep 01 00:00:00 UTC 2020

MEK inhibitor enhanced the antitumor effect of oxaliplatin and 5‑fluorouracil in MEK1 Q56P‑mutant colorectal cancer cells.

Molecular medicine reports

Jing C,Li H,Du Y,Cao H,Liu S,Wang Z,Ma R,Feng J,Wu J

Fri Feb 01 00:00:00 UTC 2019

MEK inhibitor enhanced the antitumor effect of oxaliplatin and 5‑fluorouracil in MEK1 Q56P‑mutant colorectal cancer cells.

Molecular medicine reports

Jing C,Li H,Du Y,Cao H,Liu S,Wang Z,Ma R,Feng J,Wu J

Fri Feb 01 00:00:00 UTC 2019

Dehydrogenase/reductase SDR family member 2 silencing sensitizes an oxaliplatin‑resistant cell line to oxaliplatin by inhibiting excision repair cross‑complementing group 1 protein expression.

Oncology reports

Li JM,Jiang GM,Zhao L,Yang F,Yuan WQ,Wang H,Luo YQ

Fri Nov 01 00:00:00 UTC 2019

CLEC4M is associated with poor prognosis and promotes cisplatin resistance in NSCLC patients.

Journal of Cancer

Tan LM,Li X,Qiu CF,Zhu T,Hu CP,Yin JY,Zhang W,Zhou HH,Liu ZQ

Thu Oct 01 00:00:00 UTC 2020

Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.

Journal of cellular and molecular medicine

Gao X,Wang J,Li M,Wang J,Lv J,Zhang L,Sun C,Ji J,Yang W,Zhao Z,Mao W

Tue Oct 01 00:00:00 UTC 2019

PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.

Cell death & disease

Shi WK,Zhu XD,Wang CH,Zhang YY,Cai H,Li XL,Cao MQ,Zhang SZ,Li KS,Sun HC

Sun Apr 01 00:00:00 UTC 2018

PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.

Cell death & disease

Shi WK,Zhu XD,Wang CH,Zhang YY,Cai H,Li XL,Cao MQ,Zhang SZ,Li KS,Sun HC

Sun Apr 01 00:00:00 UTC 2018

Chemosensitizing effect of shRNA-mediated ERCC1 silencing on a Xuanwei lung adenocarcinoma cell line and its clinical significance.

Oncology reports

Wang W,Zhang L,Liu L,Zheng Y,Zhang Y,Yang S,Shi R,Wang S

Sat Apr 01 00:00:00 UTC 2017

Chemosensitizing effect of shRNA-mediated ERCC1 silencing on a Xuanwei lung adenocarcinoma cell line and its clinical significance.

Oncology reports

Wang W,Zhang L,Liu L,Zheng Y,Zhang Y,Yang S,Shi R,Wang S

Sat Apr 01 00:00:00 UTC 2017

Chemosensitizing effect of shRNA-mediated ERCC1 silencing on a Xuanwei lung adenocarcinoma cell line and its clinical significance.

Oncology reports

Wang W,Zhang L,Liu L,Zheng Y,Zhang Y,Yang S,Shi R,Wang S

Sat Apr 01 00:00:00 UTC 2017

Chemosensitizing effect of shRNA-mediated ERCC1 silencing on a Xuanwei lung adenocarcinoma cell line and its clinical significance.

Oncology reports

Wang W,Zhang L,Liu L,Zheng Y,Zhang Y,Yang S,Shi R,Wang S

Sat Apr 01 00:00:00 UTC 2017

Aberrant methylation of nucleotide excision repair genes is associated with chronic arsenic poisoning.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals

Zhang A,Li H,Xiao Y,Chen L,Zhu X,Li J,Ma L,Pan X,Chen W,He Z

Sat Jul 01 00:00:00 UTC 2017

Functional characterization of a novel transcript of ERCC1 in chemotherapy resistance of ovarian cancer.

Oncotarget

Liu J,Zhang L,Mao P,Jiang G,Liu L,Wang J,Yang W,Owusu L,Li W

Tue Oct 17 00:00:00 UTC 2017

Functional characterization of a novel transcript of ERCC1 in chemotherapy resistance of ovarian cancer.

Oncotarget

Liu J,Zhang L,Mao P,Jiang G,Liu L,Wang J,Yang W,Owusu L,Li W

Tue Oct 17 00:00:00 UTC 2017

Establishment of a first-line second-line treatment model for human pulmonary adenocarcinoma.

Oncology letters

Wang L,Wang Y,Guan Q,Liu Y,He T,Wang J

Thu Dec 01 00:00:00 UTC 2016

Establishment of a first-line second-line treatment model for human pulmonary adenocarcinoma.

Oncology letters

Wang L,Wang Y,Guan Q,Liu Y,He T,Wang J

Thu Dec 01 00:00:00 UTC 2016

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 UTC 2015

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing 1; excision repair cross-complementing rodent repair deficiency, complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)

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Gene Aliases: COFS4; Ercc-1; ERCC1; RAD10; UV20

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UniProt ID: (Human) B3KRR0, (Mouse) P07903

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Entrez Gene ID: (Human) 2067, (Rat) 292673, (Mouse) 13870

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Function(s)

single-stranded DNA endodeoxyribonuclease activity TFIID-class transcription factor binding damaged DNA binding single-stranded DNA binding protein binding protein C-terminus binding protein domain specific binding structure-specific DNA binding molecular_function TBP-class protein binding DNA binding nuclease activity endonuclease activity hydrolase activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ERCC1 Polyclonal Antibody

ERCC1 Antibody (14586-1-AP) in ICC/IF

Product Details

14586-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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