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Invitrogen
ERF Polyclonal Antibody
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FIGURE: 1 / 8
ERF Antibody (PA5-30237) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of ERF was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ERF Polyclonal Antibody (Product # PA5-30237) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Product Details
PA5-30237
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ERF. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.24 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547711
Product Specific Information
Recommended positive controls: A431, ERF-transfected 293T.
Predicted reactivity: Mouse (99%), Rat (99%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Est2 repressor factor; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE 2; PE-2
Gene Aliases: CRS4; ERF; PE-2; PE2
UniProt ID: (Human) P50548, (Mouse) P70459
Entrez Gene ID: (Human) 2077, (Mouse) 13875
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
ectodermal cell differentiation
trophoblast giant cell differentiation
chorio-allantoic fusion
cell cycle
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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