Invitrogen

ERK1/ERK2 Polyclonal Antibody

15 Published Figures

28 References

View all (85) ERK1/2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Published Figures (15)

ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

ERK1/ERK2 Antibody (44-654G) in ICC/IF

Immunofluorescent analysis of ERK1 + ERK2 Antibody was done on 70% confluent log phase U87-MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ERK1 + ERK2 Antibody (Product # 44-654G) at 1:250 dilution in 1% BSA and incubated for 3 hours at room tempe... View More

ERK1/ERK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

44-654G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 26 publications 26 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Dog, Human, Insect, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antibody was produced using a synthetic peptide derived from amino acids 317-339 within the C-terminal half of the human ERK1 protein; the sequence is conserved in human and rat

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533710

Product Specific Information

For western blotting applications, we recommend using the antibody at a 1:1,000 starting dilution. Positive controls used in western blotting were human epidermoid carcinoma A431, mouse NIH3T3, and rat pheochromocytoma (PC12) cells.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Using the Culex pipiens sperm proteome to identify elements essential for mosquito reproduction.

PloS one

Thaler CD,Carstens K,Martinez G,Stephens K,Cardullo RA

44-654G was used in Western Blot to highlight subsets of proteins related to flagellar structure and sperm motility and compare the identified protein components to previous studies examining essential functions of sperm.

Wed Feb 22 00:00:00 UTC 2023

Changes of RAS Pathway Phosphorylation in Lymphoblastoid Cell Lines from Noonan Syndrome Patients Carrying Hypomorphic Variants in Two NS Genes.

International journal of molecular sciences

Tritto V,Capitanio D,Gelfi C,Riva P

44-654G was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that the presence of one subclinical variant can activate the RAS pathway below the pathological threshold, which can instead be exceeded by the additive effect due to the co-presence of two subclinical variants causing NS, supporting our digenic inheritance hypothesis.

Fri Feb 17 00:00:00 UTC 2023

Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis.

Frontiers in cell and developmental biology

Roversi FM,Bueno MLP,Pericole FV,Saad STO

44-654G was used in Western Blot to study the role of one SFK member, the Hematopoietic Cell Kinase (HCK), in CXCL12/CXCR4 pathway and its contribution to the AML pathogenesis.

Tue Apr 13 00:00:00 UTC 2021

Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis.

Frontiers in cell and developmental biology

Roversi FM,Bueno MLP,Pericole FV,Saad STO

44-654G was used in Western Blot to study the role of one SFK member, the Hematopoietic Cell Kinase (HCK), in CXCL12/CXCR4 pathway and its contribution to the AML pathogenesis.

Tue Apr 13 00:00:00 UTC 2021

Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages.

Redox biology

Guo C,Davies MJ,Hawkins CL

44-654G was used in Western Blotting to provide further insight into the cellular pathways by which SCN- could exert protective effects on supplementation to decrease the development of chronic inflammatory diseases, such as atherosclerosis.

Tue Sep 01 00:00:00 UTC 2020

Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages.

Redox biology

Guo C,Davies MJ,Hawkins CL

Tue Sep 01 00:00:00 UTC 2020

ZO-1 protein is required for hydrogen peroxide to increase MDCK cell paracellular permeability in an ERK 1/2-dependent manner.

American journal of physiology. Cell physiology

Bilal S,Jaggi S,Janosevic D,Shah N,Teymour S,Voronina A,Watari J,Axis J,Amsler K

44-654G was used in Western Blotting to demonstrate that H2O2 increases MDCK cell paracellular permeability through activation of ERK 1/2.

Sat Sep 01 00:00:00 UTC 2018

Neonatal maternal deprivation impairs localized de novo activity-induced protein translation at the synapse in the rat hippocampus.

Bioscience reports

Ahmad F,Salahuddin M,Alsamman K,Herzallah HK,Al-Otaibi ST

44-654G was used in Western Blotting to elucidate the molecular mechanisms underlying the detrimental alterations in brain functions induced by early-life stress.

Fri Jun 29 00:00:00 UTC 2018

Development of MK-8353, an orally administered ERK1/2 inhibitor, in patients with advanced solid tumors.

JCI insight

Moschos SJ,Sullivan RJ,Hwu WJ,Ramanathan RK,Adjei AA,Fong PC,Shapira-Frommer R,Tawbi HA,Rubino J,Rush TS,Zhang D,Miselis NR,Samatar AA,Chun P,Rubin EH,Schiller J,Long BJ,Dayananth P,Carr D,Kirschmeier P,Bishop WR,Deng Y,Cooper A,Shipps GW,Moreno BH,Robert L,Ribas A,Flaherty KT

Published figure using ERK1/ERK2 polyclonal antibody (Product # 44-654G) in Western Blot

Thu Feb 22 00:00:00 UTC 2018

Metformin exerts multitarget antileukemia activity in JAK2^V617F-positive myeloproliferative neoplasms.

Cell death & disease

Machado-Neto JA,Fenerich BA,Scopim-Ribeiro R,Eide CA,Coelho-Silva JL,Dechandt CRP,Fernandes JC,Rodrigues Alves APN,Scheucher PS,Simões BP,Alberici LC,de Figueiredo Pontes LL,Tognon CE,Druker BJ,Rego EM,Traina F

Published figure using ERK1/ERK2 polyclonal antibody (Product # 44-654G) in Western Blot

Thu Feb 22 00:00:00 UTC 2018

Hematopoietic cell kinase (HCK) is a potential therapeutic target for dysplastic and leukemic cells due to integration of erythropoietin/PI3K pathway and regulation of erythropoiesis: HCK in erythropoietin/PI3K pathway.

Biochimica et biophysica acta. Molecular basis of disease

Roversi FM,Pericole FV,Machado-Neto JA,da Silva Santos Duarte A,Longhini AL,Corrocher FA,Palodetto B,Ferro KP,Rosa RG,Baratti MO,Verjovski-Almeida S,Traina F,Molinari A,Botta M,Saad ST

44654G was used in western blot to characterize a new specific pharmacological hematopoietic cell kinase inhibitor

Wed Feb 01 00:00:00 UTC 2017

WNK1 kinase balances T cell adhesion versus migration in vivo.

Nature immunology

Köchl R,Thelen F,Vanes L,Brazão TF,Fountain K,Xie J,Huang CL,Lyck R,Stein JV,Tybulewicz VL

44654G was used in western blot to discover that WNK1 kinase regulates integrin-mediated adhesion and T cell migration

Thu Sep 01 00:00:00 UTC 2016

WNK1 kinase balances T cell adhesion versus migration in vivo.

Nature immunology

Köchl R,Thelen F,Vanes L,Brazão TF,Fountain K,Xie J,Huang CL,Lyck R,Stein JV,Tybulewicz VL

44654G was used in western blot to discover that WNK1 kinase regulates integrin-mediated adhesion and T cell migration

Thu Sep 01 00:00:00 UTC 2016

Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi.

International journal of molecular sciences

Margaryan NV,Gilgur A,Seftor EA,Purnell C,Arva NC,Gosain AK,Hendrix MJ,Strizzi L

44-654G was used in western blot to research pediatric large congenital melanocytic nevi to show melanocytes affect nodal expression and signaling in melanoma cells

Tue Mar 22 00:00:00 UTC 2016

Simultaneous Activation of Induced Heterodimerization between CXCR4 Chemokine Receptor and Cannabinoid Receptor 2 (CB2) Reveals a Mechanism for Regulation of Tumor Progression.

The Journal of biological chemistry

Coke CJ,Scarlett KA,Chetram MA,Jones KJ,Sandifer BJ,Davis AS,Marcus AI,Hinton CV

44-654G was used in western blot to determine a mechanism for regulation of tumor progression due to simultaneous activation of induced heterodimerization between cannabinoid receptor 2 (CB2) and CXCR4 chemokine receptor

Fri May 06 00:00:00 UTC 2016

ANKHD1 silencing inhibits Stathmin 1 activity, cell proliferation and migration of leukemia cells.

Biochimica et biophysica acta

Machado-Neto JA,Lazarini M,Favaro P,de Melo Campos P,Scopim-Ribeiro R,Franchi Junior GC,Nowill AE,Lima PR,Costa FF,Benichou S,Olalla Saad ST,Traina F

44-654G was used in western blot to identify binding partners ANKHD1 in leukemia cells.

Sun Mar 01 00:00:00 UTC 2015

Effects of a novel Nodal-targeting monoclonal antibody in melanoma.

Oncotarget

Strizzi L,Sandomenico A,Margaryan NV,Focà A,Sanguigno L,Bodenstine TM,Chandler GS,Reed DW,Gilgur A,Seftor EA,Seftor RE,Khalkhali-Ellis Z,Leonardi A,Ruvo M,Hendrix MJ

44-654G was used in western blot to assess the use of 3D1 mAb for treating Nodal expressing cancers.

Tue Oct 27 00:00:00 UTC 2015

Candidate tumor suppressor B-cell translocation gene 3 impedes neoplastic progression by suppression of AKT.

Cell death & disease

Cheng YC,Chen PH,Chiang HY,Suen CS,Hwang MJ,Lin TY,Yang HC,Lin WC,Lai PL,Shieh SY

44-654G was used in western blot to elucidate the tumor suppressor function of BTG3

Thu Jan 08 00:00:00 UTC 2015

Increased ERK and JNK activation and decreased ERK/JNK ratio are associated with long-term organ damage in patients with systemic lupus erythematosus.

Rheumatology (Oxford, England)

Bloch O,Amit-Vazina M,Yona E,Molad Y,Rapoport MJ

44-654G was used in western blot to test if extracellular signal-regulated kinase and c-Jun N-terminal kinase are associated with long-term organ damage in SLE patients.

Sun Jun 01 00:00:00 UTC 2014

Silver and fullerene nanoparticles' effect on interleukin-2-dependent proliferation of CD4 (+) T cells.

Toxicology in vitro : an international journal published in association with BIBRA

Côté-Maurais G,Bernier J

44-654G was used in western blot to determine if nanoparticles inhibit IL-2-dependent T cell proliferation.

Mon Dec 01 00:00:00 UTC 2014

Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression.

The Journal of cell biology

Lim ST,Miller NL,Chen XL,Tancioni I,Walsh CT,Lawson C,Uryu S,Weis SM,Cheresh DA,Schlaepfer DD

Published figure using ERK1/ERK2 polyclonal antibody (Product # 44-654G) in Western Blot

Mon Jun 25 00:00:00 UTC 2012

Lyn is a redox sensor that mediates leukocyte wound attraction in vivo.

Nature

Yoo SK,Starnes TW,Deng Q,Huttenlocher A

44-654G was used in western blot to identify Lyn as a redox sensor that recruits neutrophils to wounds in zebrafish larvae.

Sun Nov 20 00:00:00 UTC 2011

Increased ERK and JNK activities correlate with disease activity in patients with systemic lupus erythematosus.

Annals of the rheumatic diseases

Molad Y,Amit-Vasina M,Bloch O,Yona E,Rapoport MJ

44-654G was used in western blot to discuss the contribution of the p21ras/MAP kinase pathway to systemic lupus erythematosus

Fri Jan 01 00:00:00 UTC 2010

Alternative modes of GM-CSF receptor activation revealed using activated mutants of the common beta-subunit.

Blood

Perugini M,Brown AL,Salerno DG,Booker GW,Stojkoski C,Hercus TR,Lopez AF,Hibbs ML,Gonda TJ,D'Andrea RJ

Thu Apr 22 00:00:00 UTC 2010

Alternative modes of GM-CSF receptor activation revealed using activated mutants of the common beta-subunit.

Blood

Perugini M,Brown AL,Salerno DG,Booker GW,Stojkoski C,Hercus TR,Lopez AF,Hibbs ML,Gonda TJ,D'Andrea RJ

Thu Apr 22 00:00:00 UTC 2010

The absence of caveolin-1 increases proliferation and anchorage- independent growth by a Rac-dependent, Erk-independent mechanism.

Molecular and cellular biology

Cerezo A,Guadamillas MC,Goetz JG,Sánchez-Perales S,Klein E,Assoian RK,del Pozo MA

44-654G was used in western blot to elucidate the mechanisms by which loss of Cav1 function promotes cancer cell growth

Tue Sep 01 00:00:00 UTC 2009

Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling.

Journal of leukocyte biology

Ratthé C,Pelletier M,Chiasson S,Girard D

Tue May 01 00:00:00 UTC 2007

The mitogen-activated protein kinases (MAPK) p38 and JNK are markers of tumor progression in breast carcinoma.

Gynecologic oncology

Davidson B,Konstantinovsky S,Kleinberg L,Nguyen MT,Bassarova A,Kvalheim G,Nesland JM,Reich R

Fri Sep 01 00:00:00 UTC 2006

Phospho-caveolin-1 mediates integrin-regulated membrane domain internalization.

Nature cell biology

del Pozo MA,Balasubramanian N,Alderson NB,Kiosses WB,Grande-García A,Anderson RG,Schwartz MA

Thu Sep 01 00:00:00 UTC 2005

Phospho-caveolin-1 mediates integrin-regulated membrane domain internalization.

Nature cell biology

del Pozo MA,Balasubramanian N,Alderson NB,Kiosses WB,Grande-García A,Anderson RG,Schwartz MA

Thu Sep 01 00:00:00 UTC 2005

TNF-alpha promotes a stop signal that inhibits neutrophil polarization and migration via a p38 MAPK pathway.

Journal of leukocyte biology

Lokuta MA,Huttenlocher A

Fri Jul 01 00:00:00 UTC 2005

Matrix metalloproteinases (MMP), EMMPRIN (extracellular matrix metalloproteinase inducer) and mitogen-activated protein kinases (MAPK): co-expression in metastatic serous ovarian carcinoma.

Clinical & experimental metastasis

Davidson B,Givant-Horwitz V,Lazarovici P,Risberg B,Nesland JM,Trope CG,Schaefer E,Reich R

Thu Dec 18 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein tyrosine kinase ERK2

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Gene Aliases: 9030612K14Rik; AA407128; AU018647; BOS_16809; BOS_22892; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

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UniProt ID: (Bovine) P46196, (Human) P28482, (Human) P27361, (Mouse) P63085, (Rat) P63086, (Rat) P21708, (Mouse) Q63844

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Entrez Gene ID: (Bovine) 327672, (Human) 5594, (Bovine) 531391, (Human) 5595, (Mouse) 26413, (Rat) 116590, (Rat) 50689, (Mouse) 26417

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Function(s)

phosphotyrosine binding protein serine/threonine kinase activity MAP kinase activity ATP binding RNA polymerase II carboxy-terminal domain kinase activity phosphatase binding DNA binding protein binding transcription factor binding kinase activity mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ERK1/ERK2 Polyclonal Antibody

ERK1/ERK2 Antibody (44-654G) in ICC/IF

Product Details

44-654G

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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