Invitrogen

ERK1/ERK2 Recombinant Superclonal™ Antibody (3HCLC)

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View all (85) ERK1/2 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

710214

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
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ChIP assay (ChIP)

1 µL
-
Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

3HCLC

Immunogen

Peptide corresponding to amino acids 293-315 of human extracellular signal regulated kinase 1/2
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532630

Product Specific Information

This antibody is predicted to react with equine, mouse and rat based on sequence homology.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44-ERK1; p44-MAPK; protein tyrosine kinase ERK2

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Gene Aliases: ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

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UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Rat) P21708

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Entrez Gene ID: (Human) 5594, (Human) 5595, (Rat) 116590, (Rat) 50689

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Function(s)
phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding protein kinase activity protein kinase binding
Process(es)
MAPK cascade MAPK import into nucleus positive regulation of protein phosphorylation transcription, DNA-templated protein complex assembly protein phosphorylation apoptotic process cellular response to DNA damage stimulus DNA damage induced protein phosphorylation response to toxic substance organ morphogenesis phosphorylation peptidyl-serine phosphorylation sensory perception of pain arachidonic acid metabolic process BMP signaling pathway lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide regulation of stress-activated MAPK cascade positive regulation of histone phosphorylation positive regulation of histone acetylation intracellular signal transduction peptidyl-tyrosine autophosphorylation response to exogenous dsRNA positive regulation of translation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of sequence-specific DNA binding transcription factor activity cartilage development regulation of cytoskeleton organization lung morphogenesis trachea formation positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus caveolin-mediated endocytosis regulation of Golgi inheritance regulation of early endosome to late endosome transport negative regulation of apolipoprotein binding activation of MAPK activity transcription initiation from RNA polymerase I promoter cell cycle axon guidance fibroblast growth factor receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling viral process platelet activation thyroid gland development positive regulation of cyclase activity positive regulation of telomere maintenance via telomerase Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis outer ear morphogenesis thymus development positive regulation of telomerase activity Bergmann glial cell differentiation face development JAK-STAT cascade involved in growth hormone signaling pathway cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade regulation of cellular response to heat positive regulation of telomere capping positive regulation of xenophagy chemotaxis response to stress signal transduction chemical synaptic transmission positive regulation of cell proliferation positive regulation of peptidyl-threonine phosphorylation peptidyl-threonine phosphorylation cytosine metabolic process positive regulation of cell migration regulation of protein stability mammary gland epithelial cell proliferation ERBB signaling pathway response to estrogen negative regulation of cell differentiation T cell receptor signaling pathway B cell receptor signaling pathway long-term synaptic potentiation labyrinthine layer blood vessel development cellular response to granulocyte macrophage colony-stimulating factor stimulus cellular response to organic substance
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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