Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ERK1/2 Antibodies

          Invitrogen

          ERK1/ERK2 Monoclonal Antibody (K.913.4)

          Advanced Verification
          4 Published Figures
          9 References
          View all (85) ERK1/2 antibodies

          Promotion

          View available promotion(s)

          Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite ERK1/ERK2 Monoclonal Antibody (K.913.4)

          • Antibody Testing Data (7)
          • Published Figures (4)
          • Advanced Verification (3)
          ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          ERK1/ERK2 Antibody (MA5-15134) in ICC/IF

          Immunofluorescence analysis of ERK1/ERK2 was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ERK1/ERK2 Rabbit Monoclonal Antibody(K.913.4) (Product # MA5-15134) at 1:100 dilution in 0.1% BSA, incubated at ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
          ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
          ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
          ERK1/ERK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Flow Cytometry (Flow)
          ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody
          ERK1/ERK2 Antibody
          ERK1/ERK2 Antibody

          Product Details

          MA5-15134

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 6 publications 6 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:125-1:500
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          1:200-1:800
          -

          Immunoprecipitation (IP)

          1:50
          -
          Product Specifications

          Species Reactivity

          Bovine, Dog, C. elegans, Fruit fly, Hamster, Human, Mink, Mouse, Non-human primate, Pig, Rat, Zebrafish

          Published species

          Chicken, Human, Pig, Rat, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Monoclonal

          Type

          Antibody

          Clone

          K.913.4

          Immunogen

          A synthetic peptide corresponding to residues near the C-terminus of human p44 MAP kinase.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          84 µg/mL

          Purification

          Affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          <0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10982335

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with JNK/SAPK or p38 MAP kinase.

          Target Information

          ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; Diphospho-ERK; drosophila ERK; enhancer of seven in absentia 7; ERK; Erk MAP kinase; ERK-1; ERK-2; ERK/rolled; erk1 erk2; ERK1b; ERK2; ERT1; ERT2; extracellular signal-regulated kinase; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; Insulin-stimulated MAP2 kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAP-kinase; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; phospho-ERK; pp42/MAP kinase; protein kinase; Protein rolled; protein tyrosine kinase ERK2; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; rolled; sevenmaker

          View more View less

          Gene Aliases: 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; BOS_16809; BOS_22892; C78273; CG12559; CG18732; CT34260; CT39192; D-ERK; DERK; DERK-A; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmErk; DmERK-A; DmERKA; DmMAPK; dp-ERK; DpERK; dpERK1; dpMAPK; Dsor2; E(sina)7; EC2-1; EK2-1; ERK; ERK-1; ERK-2; ERK-A; Erk/Map kinase; ERK1; ERK2; ERKA; ERT1; ERT2; Esrk1; EY2-2; fi06b09; GroupII; HS44KDAP; HUMKER1A; I79_009500; I79_018350; l(2)41Ac; l(2R)EMS45-39; MAP-k; MAPK; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; pERK; pMAPK; PRKM1; PRKM2; PRKM3; RL; rl/ERK; rl/MAPK; rll; SEM; SR2-1; Su(Raf)2B; wu:fi06b09; zERK1; zERK2

          View more View less

          UniProt ID: (Human) P28482, (Bovine) P46196, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708, (Fruit fly) P40417

          View more View less

          Entrez Gene ID: (Human) 5594, (Dog) 477575, (Bovine) 327672, (Pig) 100153927, (Bovine) 531391, (Human) 5595, (Dog) 100855877, (Chinese hamster) 100759514, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689, (Chinese hamster) 100753149, (Zebrafish) 360144, (Zebrafish) 399480, (Fruit fly) 3354888

          View more View less

          Function(s)
          phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding protein kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups JUN kinase activity
          Process(es)
          MAPK cascade activation of MAPK activity MAPK import into nucleus positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly protein phosphorylation apoptotic process DNA damage induced protein phosphorylation cell cycle axon guidance fibroblast growth factor receptor signaling pathway response to toxic substance regulation of phosphatidylinositol 3-kinase signaling viral process phosphorylation peptidyl-serine phosphorylation sensory perception of pain arachidonic acid metabolic process platelet activation BMP signaling pathway thyroid gland development positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase regulation of stress-activated MAPK cascade positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis outer ear morphogenesis response to exogenous dsRNA positive regulation of translation positive regulation of transcription from RNA polymerase II promoter thymus development regulation of sequence-specific DNA binding transcription factor activity cartilage development regulation of cytoskeleton organization positive regulation of telomerase activity Bergmann glial cell differentiation face development JAK-STAT cascade involved in growth hormone signaling pathway lung morphogenesis trachea formation cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus caveolin-mediated endocytosis regulation of Golgi inheritance regulation of cellular response to heat positive regulation of telomere capping positive regulation of xenophagy regulation of early endosome to late endosome transport negative regulation of apolipoprotein binding eye development brain development regulation of gene expression central nervous system projection neuron axonogenesis intracellular signal transduction chordate embryonic development cellular response to organic substance transcription, DNA-templated chemotaxis response to stress cellular response to DNA damage stimulus signal transduction chemical synaptic transmission positive regulation of cell proliferation positive regulation of peptidyl-threonine phosphorylation peptidyl-threonine phosphorylation cytosine metabolic process positive regulation of cell migration regulation of protein stability mammary gland epithelial cell proliferation ERBB signaling pathway response to estrogen negative regulation of cell differentiation positive regulation of transcription, DNA-templated T cell receptor signaling pathway B cell receptor signaling pathway long-term synaptic potentiation labyrinthine layer blood vessel development cellular response to granulocyte macrophage colony-stimulating factor stimulus organ morphogenesis response to lipopolysaccharide regulation of transcription, DNA-templated mitotic nuclear division transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway gastrulation imaginal disc-derived wing vein specification imaginal disc-derived wing morphogenesis heart development metamorphosis torso signaling pathway determination of adult lifespan imaginal disc-derived wing vein morphogenesis anterior/posterior axis specification, embryo cellular response to starvation negative regulation of macroautophagy adherens junction maintenance cellular response to reactive oxygen species negative regulation of apoptotic process R7 cell development sevenless signaling pathway positive regulation of photoreceptor cell differentiation positive regulation of Ras protein signal transduction behavioral response to ethanol regulation of synapse structure or activity modulation of synaptic transmission cellular response to arsenic-containing substance cellular response to cadmium ion positive regulation of wound healing regulation of heart morphogenesis regulation of response to drug
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-67c77f6dc7-n6nf2:80/100.66.135.186:80.
          git-commit: 545e792480003146e539d624bd2a21b51d198228
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.31.0-2025.06.23-1.0