Invitrogen

ERK2 Recombinant Rabbit Monoclonal Antibody (SZ25-01)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-32025

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SZ25-01

Immunogen

Synthetic peptide within Human ERK2 aa 311-360
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809319

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ERK-2; ERT1; Extracellular signal-regulated kinase 2; extracellular-signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; mitogen activated protein kinase 1; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p42-MAPK; protein tyrosine kinase ERK2

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Gene Aliases: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-2; ERK2; ERT1; Mapk; MAPK1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2

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UniProt ID: (Human) P28482, (Mouse) P63085, (Rat) P63086

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Entrez Gene ID: (Human) 5594, (Mouse) 26413, (Rat) 116590

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Function(s)
phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding
Process(es)
MAPK cascade MAPK import into nucleus transcription, DNA-templated protein phosphorylation apoptotic process cellular response to DNA damage stimulus cell cycle signal transduction response to toxic substance organ morphogenesis positive regulation of peptidyl-threonine phosphorylation phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation sensory perception of pain cytosine metabolic process lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide regulation of stress-activated MAPK cascade mammary gland epithelial cell proliferation intracellular signal transduction ERBB signaling pathway response to exogenous dsRNA response to estrogen negative regulation of cell differentiation positive regulation of translation T cell receptor signaling pathway B cell receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity regulation of cytoskeleton organization long-term synaptic potentiation lung morphogenesis trachea formation labyrinthine layer blood vessel development ERK1 and ERK2 cascade response to epidermal growth factor cellular response to organic substance caveolin-mediated endocytosis regulation of Golgi inheritance cellular response to granulocyte macrophage colony-stimulating factor stimulus regulation of early endosome to late endosome transport activation of MAPK activity chemotaxis response to stress chemical synaptic transmission axon guidance positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling viral process platelet activation positive regulation of cell migration thyroid gland development regulation of protein stability positive regulation of telomere maintenance via telomerase Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis outer ear morphogenesis positive regulation of transcription, DNA-templated thymus development positive regulation of telomerase activity Bergmann glial cell differentiation face development JAK-STAT cascade involved in growth hormone signaling pathway cardiac neural crest cell development involved in heart development regulation of cellular response to heat positive regulation of telomere capping
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