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Invitrogen
ERLIN2 Monoclonal Antibody (3H9A2)
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FIGURE: 1 / 7
ERLIN2 Antibody (MA5-49296) in ICC/IF
Immunocytochemistry analysis of ERLIN2 in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in ERLIN2 Monoclonal antibody (Product # MA5-49296) using a dilution of 5 μg/mL. Cy3 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the ... View More
Product Details
MA5-49296
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3H9A2
Immunogen
E.coli-derived human Erlin-2/ERLIN2 recombinant protein (Position: D87-N339).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074954
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human T47D whole cell, human HCCT tissue, human HCCP tissue. IHC: human ovarian serous carcinoma tissue, human placenta tissue, human squamous cell lung carcinoma tissue, human thyroiditis tissue. ICC/IF: T47D cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
ERLIN2, also named as C8orf2 and SPFH2, is a component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1, 4, 5-trisphosphate receptors (IP3Rs) such as ITPR1. It promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex. ERLIN2 is involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. The MW of ERLIN1/ERLIN2 complex is about 70 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Endoplasmic reticulum lipid raft-associated protein 2; Erlin-2; HGNC:1356; MGC87072; SPFH domain family, member 2; SPFH domain-containing protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2; UNQ2441
Gene Aliases: C8orf2; Erlin-2; ERLIN2; NET32; SPFH2; SPG18; UNQ2441/PRO5003/PRO9924
UniProt ID: (Human) O94905
Entrez Gene ID: (Human) 11160
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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