Invitrogen

ERN1/ERN2 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-115434

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ERN1(Accession O75460), corresponding to amino acid residues P603-V653.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2900070

Product Specific Information

Antibody detects endogenous levels of total IRE1.

Target Information

ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1) is a Protein Coding gene. Diseases associated with ERN1 include Arrhythmogenic Right Ventricular Dysplasia, Familial, 5 and Epiphyseal Dysplasia, Multiple, With Early-Onset Diabetes Mellitus. Among its related pathways are Spinocerebellar ataxia and Photodynamic therapy-induced unfolded protein response. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is ERN2. This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: endoplasmic reticulum (ER) to nucleus signalling 1; endoplasmic reticulum (ER) to nucleus signalling 2; endoplasmic reticulum to nucleus signalling 2; Endoplasmic reticulum-to-nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 2; ER to nucleus signalling 1; ER to nucleus signalling 2; ERN1; ERN2; hIRE1p; hIRE2p; inositol-requiring 1; inositol-requiring 1 (Yeast homologue); inositol-requiring 1 alpha; inositol-requiring 1 beta; inositol-requiring enzyme 1; Inositol-requiring protein 1; Inositol-requiring protein 2; IRE1; IRE1 beta; IRE1, S. cerevisiae, homolog of; Ire1-alpha; Ire1-beta; IRE1a; IRE1b; IRE2; mIre1; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1; Serine/threonine-protein kinase/endoribonuclease IRE2

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Gene Aliases: 9030414B18Rik; AI225830; C85377; ERN1; ERN2; hIRE1p; hIRE2p; IRE1; IRE1-BETA; IRE1a; Ire1alpha; IRE1b; IRE1P; IRE2; IRE2p; RGD1559716

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UniProt ID: (Human) O75460, (Human) Q76MJ5, (Mouse) Q9EQY0, (Mouse) Q9Z2E3

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Entrez Gene ID: (Human) 2081, (Human) 10595, (Mouse) 78943, (Rat) 498013, (Mouse) 26918, (Rat) 365363

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Function(s)
magnesium ion binding endoribonuclease activity protein serine/threonine kinase activity protein binding ATP binding enzyme binding Hsp70 protein binding identical protein binding protein homodimerization activity ADP binding Hsp90 protein binding endonuclease activity ribonuclease activity nucleotide binding catalytic activity protein kinase activity kinase activity bidentate ribonuclease III activity transferase activity transferase activity, transferring phosphorus-containing groups hydrolase activity metal ion binding
Process(es)
transcription, DNA-templated mRNA processing protein phosphorylation rRNA catabolic process apoptotic chromosome condensation response to endoplasmic reticulum stress negative regulation of transcription, DNA-templated RNA phosphodiester bond hydrolysis endothelial cell proliferation regulation of transcription, DNA-templated mRNA cleavage apoptotic process response to unfolded protein activation of signaling protein activity involved in unfolded protein response cell cycle arrest activation of JUN kinase activity metabolic process phosphorylation endoplasmic reticulum unfolded protein response mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response positive regulation of RNA splicing cellular response to vascular endothelial growth factor stimulus peptidyl-serine autophosphorylation IRE1-mediated unfolded protein response protein autophosphorylation mRNA splicing, via endonucleolytic cleavage and ligation mRNA endonucleolytic cleavage involved in unfolded protein response intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to glucose stimulus RNA phosphodiester bond hydrolysis, endonucleolytic positive regulation of endoplasmic reticulum unfolded protein response insulin metabolic process peptidyl-serine trans-autophosphorylation mRNA catabolic process regulation of macroautophagy nucleic acid phosphodiester bond hydrolysis RNA splicing
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