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Invitrogen
ERO1L Recombinant Rabbit Monoclonal Antibody (11H3L5)
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FIGURE: 1 / 5
ERO1L Antibody (702709) in ICC/IF
For immunofluorescence analysis, SW480 cells were fixed and permeabilized for detection of endogenous ERO1 using Anti- ERO1 Recombinant Rabbit Monoclonal Antibody (Product # 702709, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of ERO1 (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel... View More
Product Details
702709
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
11H3L5
Immunogen
Protein corresponding to human ERO1L (aa22-aa468)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2716886
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Bovine
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in ER membrane as peripheral membrane protein on the lumenal side. Its association with ERP44 is essential for its retention in the ER and is unique in coupling oxygen reduction to de novo disulfides formation. The ERO1L enzyme is broadly distributed at low levels in several tissues with highest levels in the upper digestive tract and is stimulated by hypoxia via HIF-signaling. ERO1L generally exists as monomer, but has the ability to function as monomer or homodimer and interacts with PDILT. After translation, ERO1L gets n-glycosylated and the disulfide bonds constitute the redox-active center whereby the Cys-94/Cys-99 disulfide bond accepts an electron from P4HB followed by funneling the same to the active site disulfide Cys-394/Cys-397. ERO1L acts by oxidizing directly to P4HB/PDI isomerase through direct disulfide exchange and associates with ERP44. ERO1L is implicated in immunoglobulin folding, oxidative stress, release of unfolded cholera toxin from reduced P4HB/PDI upon Vibrio cholerae infection, and plays a key role in ER stress-induced CHOP-dependent apoptosis through inositol 1,4,5-trisphosphate receptor IP3R1 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Endoplasmic oxidoreductin-1-like protein; endoplasmic reticulum oxidation 1; Endoplasmic reticulum oxidoreductase alpha; Endoplasmic reticulum oxidoreductin-1-like protein; ero1; ERO1-L; ERO1-L-alpha; ERO1-like protein alpha; Oxidoreductin-1-L-alpha
Gene Aliases: ERO1-alpha; ERO1-L; ERO1-L-alpha; ERO1A; Ero1alpha; ERO1L; ERO1LA; UNQ434/PRO865
UniProt ID: (Human) Q96HE7, (Mouse) Q8R180
Entrez Gene ID: (Human) 30001, (Mouse) 50527
protein disulfide isomerase activity
protein binding
protein disulfide oxidoreductase activity
disulfide oxidoreductase activity
oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor
peptide-methionine (S)-S-oxide reductase activity
peptide-methionine (R)-S-oxide reductase activity
methionine-R-sulfoxide reductase activity
protein folding
transport
apoptotic process
4-hydroxyproline metabolic process
protein maturation by protein folding
extracellular matrix organization
endoplasmic reticulum unfolded protein response
response to endoplasmic reticulum stress
cell redox homeostasis
brown fat cell differentiation
chaperone mediated protein folding requiring cofactor
release of sequestered calcium ion into cytosol
oxidation-reduction process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
response to reactive oxygen species
cellular protein modification process
response to temperature stimulus
organ senescence
cellular response to hypoxia
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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