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ERp57 Antibody (MA5-45229) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with Erp57 (Grp58) monoclonal antibody (Product # MA5-45229) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erp57 (Grp58) antib... View More
Product Details
MA5-45229
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Map.ERp57
Immunogen
Human recombinant ERp57 (Grp58)
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931683
Product Specific Information
0.5 µg/mL of MA5-45229 was sufficient for detection of ERp57 in 10 µg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 57kDa.
Target Information
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER-60; endoplasmic reticulum P58; endoplasmic reticulum protein; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER p57; ER protein 57; ER protein 60; ER-60 protease; ERp 57; ERp57; ERP57 protein; ERP60; glucose regulated protein 58; glucose regulated protein 58kD; glucose regulated protein, 58 kDa; glucose regulated protein, 58kDa; GRP 57; GRP 58; HIP-70; oxidoreductase ERp57; p58; PDIA 3; phospholipase C, alpha; phospholipase C-alpha; PI PLC; protein disulfide isomerase associated 3; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; Protein disulfide-isomerase A3; Q-2
Gene Aliases: 58kDa; BOS_20460; ER60; Erp; ERP57; ERP60; ERp61; grp-58; GRP57; GRP58; HEL-S-269; HEL-S-93n; HsT17083; I79_003765; P58; PDI; PDI-Q2; PDIA3; PI-PLC; PLC[a]; Plca
UniProt ID: (Human) P30101, (Mouse) P27773, (Rat) P11598
protein disulfide isomerase activity
cysteine-type endopeptidase activity
phospholipase C activity
protein binding
disulfide oxidoreductase activity
poly(A) RNA binding
electron carrier activity
protein disulfide oxidoreductase activity
peptidase activity
isomerase activity
protein-disulfide reductase (glutathione) activity
MHC class I protein binding
protein folding
proteolysis
response to endoplasmic reticulum stress
cell redox homeostasis
oxidation-reduction process
cellular response to vitamin D
positive regulation of protein folding
positive regulation of extrinsic apoptotic signaling pathway
antigen processing and presentation of peptide antigen via MHC class I
protein import into nucleus
protein retention in ER lumen
signal transduction
protein folding in endoplasmic reticulum
glycerol ether metabolic process
positive regulation of apoptotic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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