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ESRP1 Antibody (PA5-89948) in WB
Western blot analysis of ESRP1 in extracts of various cell lines. Samples were incubated with ESRP1 Polyclonal antibody (Product # PA5-89948) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-89948
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ESRP1 (NP_0601672)
Conjugate
Form
Liquid
Concentration
2.75 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805860
Product Specific Information
Immunogen sequence: MTASPDYLVV LFGITAGATG AKLGSDEKEL ILLFWKVVDL ANKKVGQLHE VLVRPDQLEL TEDCKEETKI DVESLSSASQ LDQALRQFNQ SVSNELNIGV GTSFCLCTDG QLHVRQILHP EASKKNVLLP ECFYSFFDLR KEFKKCCPGS PDIDKLDVAT MTEYLNFEKS SSVSRYGASQ VEDMGNIILA MISEPYNHRF SDPERVNYKF ESGTCSKMEL; Positive Samples: LO2, MCF7, HT-29, 293T, Mouse thymus, Mouse pancreas; Cellular Location: Nucleus
Target Information
RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Epithelial splicing regulatory protein 1; RNA binding motif protein 35A; RNA-binding motif protein 35A; RNA-binding protein 35A
Gene Aliases: 2210008M09Rik; A630065D16; BC031468; ESRP1; RBM35A; RMB35A
UniProt ID: (Human) Q6NXG1, (Mouse) Q3US41
Entrez Gene ID: (Human) 54845, (Mouse) 207920
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