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Invitrogen
ESRRA Polyclonal Antibody
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FIGURE: 1 / 3
ESRRA Antibody (PA1-314) in WB
Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of PC-3 (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3) and whole cell extracts (30 µg lysate) of SK-OV-3 (Lane 4) and PC-12 (Lane 5). The blots were probed with Anti-Estrogen Related Receptor Rabbit Polyclonal (Product # PA1-314, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 45 kDa band corresponding to Estrogen- Related Recepto... View More
Product Details
PA1-314
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the residues M(1) S S Q V V G I E P L Y I K A E(16) of human ERR1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2293666
Product Specific Information
PA1-314 detects human ERR1 from transfected cells and shows no cross-reactivity to ERR3.
PA1-314 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~52 kDa protein representing ERR1 from COS-7 transfected cells. An unidentified band is seen at ~37 kDa.
The PA1-314 immunogen is a synthetic peptide corresponding to the residues M(1) S S Q V V G I E P L Y I K A E(16) of human ERR1. This sequence is completely conserved between canine, human, mouse and rat. This immunizing peptide (PEP-231) can be used for neutralization and control experiments.
Target Information
Estrogen-related receptor alpha 1 (ERR alpha, ERR1) is an orphan member of the nuclear hormone receptor superfamily. All the ERR family members share an almost identical DNA-binding domain, which has 68% amino acid identity with that of estrogen receptor. This protein binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-tnaaggtca-3' can bind to the medium-chain acyl coenzyme a dehydrogenase (mcad) response element nrre-1 and may act as an important regulator of mcad promoter. Studies have shown that ERR1 functions a metabolic regulator and is often expressed in tissues that preferentially metabolize fatty acids. The corresponding gene for the ERR1 is NR3B1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ERR alpha; ERR-alpha; Estrogen receptor-like 1; estrogen-related nuclear receptor alpha; Estrogen-related receptor alpha; estrogen-related receptor, alpha; HERR1; Nuclear receptor subfamily 3 group B member 1; orphan nuclear receptor; Steroid hormone receptor ERR1
Gene Aliases: ERR1; ERRa; ERRalpha; Errra; ESRL1; ESRRA; NR3B1
UniProt ID: (Human) P11474, (Rat) Q5QJV7
Entrez Gene ID: (Human) 2101, (Rat) 293701
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
steroid binding
protein binding
zinc ion binding
protein domain specific binding
sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
mitochondrion organization
intracellular receptor signaling pathway
response to estradiol
regulation of cell proliferation
steroid hormone mediated signaling pathway
regulation of osteoblast differentiation
regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
positive regulation of cellular response to insulin stimulus
transcription, DNA-templated
transcription from RNA polymerase II promoter
regulation of ossification
positive regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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