Proteintech

ETFA Polyclonal Antibody

ETFA Antibody in Immunocytochemistry (ICC/IF)

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ETFA Antibody (12262-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETFA antibody (Product # 12262-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594phalloidin (red).

ETFA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

12262-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ETFA Fusion Protein Ag2931 (1-333 aa encoded by BC015526)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MFRAAAPGQ LRRAASLLRF QSTLVIAEHA NDSLAPITLN TITAATRLGG EVSCLVAGTK CDKVAQDLCK VAGIAKVLVA QHDVYKGLLP EELTPLILAT QKQFNYTHIC AGASAFGKNL LPRVAAKLEV APISDIIAIK SPDTFVRTIY AGNALCTVKC DEKVKVFSVR GTSFDAAATS GGSASSEKAS STSPVEISEW LDQKLTKSDR PELTGAKVVV SGGRGLKSGE NFKLLYDLAD QLHAAVGASR AAVDAGFVPN DMQVGQTGKI VAPELYIAVG ISGAIQHLAG MKDSKTIVAI NKDPEAPIFQ VADYGIVADL FKVVPEMTEI LKKK (1-333 aa encoded by BC015526)

Target Information

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Cellular and molecular life sciences : CMLS

Peng MZ,Shao YX,Li XZ,Zhang KD,Cai YN,Lin YT,Jiang MY,Liu ZC,Su XY,Zhang W,Jiang XL,Liu L

Tue Jun 21 00:00:00 UTC 2022

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Molecular cell

Jiang B,Zhang J,Zhao G,Liu M,Hu J,Lin F,Wang J,Zhao W,Ma H,Zhang C,Wu C,Yao L,Liu Q,Chen X,Cao Y,Zheng Y,Zhang C,Han A,Lin D,Li Q

Thu May 19 00:00:00 UTC 2022

Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis.

Journal of proteomics

Muto T,Taniguchi H,Kushima R,Tsuda H,Yonemori H,Chen C,Sugihara Y,Sakamoto K,Kobori Y,Palmer H,Nakamura Y,Tomonaga T,Tanaka H,Mizushima H,Fujita S,Kondo T

Mon May 16 00:00:00 UTC 2011

Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis.

Journal of proteomics

Muto T,Taniguchi H,Kushima R,Tsuda H,Yonemori H,Chen C,Sugihara Y,Sakamoto K,Kobori Y,Palmer H,Nakamura Y,Tomonaga T,Tanaka H,Mizushima H,Fujita S,Kondo T

Mon May 16 00:00:00 UTC 2011

Proteomic analysis of nuclei isolated from cancer cell lines treated with indenoisoquinoline NSC 724998, a novel topoisomerase I inhibitor.

Journal of proteome research

Han B,Stockwin LH,Hancock C,Yu SX,Hollingshead MG,Newton DL

Fri Aug 06 00:00:00 UTC 2010

Proteomic analysis of nuclei isolated from cancer cell lines treated with indenoisoquinoline NSC 724998, a novel topoisomerase I inhibitor.

Journal of proteome research

Han B,Stockwin LH,Hancock C,Yu SX,Hollingshead MG,Newton DL

Fri Aug 06 00:00:00 UTC 2010

Activity-based protein profiling of the hepatitis C virus replication in Huh-7 hepatoma cells using a non-directed active site probe.

Proteome science

Singaravelu R,Blais DR,McKay CS,Pezacki JP

Thu Feb 04 00:00:00 UTC 2010

Activity-based protein profiling of the hepatitis C virus replication in Huh-7 hepatoma cells using a non-directed active site probe.

Proteome science

Singaravelu R,Blais DR,McKay CS,Pezacki JP

Thu Feb 04 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: Alpha-ETF; electron transfer flavoprotein alpha-subunit; Electron transfer flavoprotein subunit alpha, mitochondrial; electron transfer flavoprotein, alpha polypeptide; electron transferring flavoprotein, alpha polypeptide; electron-transfer-flavoprotein alpha polypeptide; glutaric aciduria II

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Gene Aliases: 2010200I21Rik; D9Ertd394e; EMA; ETF; ETFA; GA2; MADD

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UniProt ID: (Human) P13804, (Mouse) Q99LC5, (Rat) P13803

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Entrez Gene ID: (Human) 2108, (Mouse) 110842, (Rat) 300726

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Function(s)

fatty-acyl-CoA binding acyl-CoA dehydrogenase activity electron carrier activity oxidoreductase activity flavin adenine dinucleotide binding oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor protein binding

Process(es)

transport oxidation-reduction process biological_process respiratory electron transport chain fatty acid beta-oxidation using acyl-CoA dehydrogenase lipid homeostasis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ETFA Polyclonal Antibody

ETFA Antibody (12262-1-AP) in ICC/IF

Product Details

12262-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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