Proteintech

ETV5 Polyclonal Antibody

ETV5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

ETV5 Antibody (13011-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed PC-3 cells using 13011-1-AP (ETV5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

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Product Details

13011-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 8 publications 8 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

Assay-dependent

View 3 publications 3 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ETV5 Fusion Protein Ag3708 (160-510 aa encoded by BC007333)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.53 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SGHAPAAGP VQGVGPAPAP HSLPEPGPQQ QTFAVPRPPH QPLQMPKMMP ENQYPSEQRF QRQLSEPCHP FPPQPGVPGD NRPSYHRQMS EPIVPAAPPP PQGFKQEYHD PLYEHGVPGM PGPPAHGFQS PMGIKQEPRD YCVDSEVPNC QSSYMRGGYF SSSHEGFSYE KDPRLYFDDT CVVPERLEGK VKQEPTMYRE GPPYQRRGSL QLWQFLVTLL DDPANAHFIA WTGRGMEFKL IEPEEVARRW GIQKNRPAMN YDKLSRSLRY YYEKGIMQKV AGERYVYKFV CDPDALFSMA FPDNQRPFLK AESECHLSEE DTLPLTHFED SPAYLLDMDR CSSLPYAEGF AY (160-510 aa encoded by BC007333)

Target Information

ETS variant 5, transcription factor of the ETS family, divergent member of the winged helix-turn-helix superfamily, expressed in brain, placenta and other tissues involved in development. Tissue specificity: Ubiquitous. ERM is expressed in Sertoli cells and spermatogonial stem cells (SSCs). Studies on knockout mice have shown that it is required for SSC self-renewal but not differentiation, thus affected mice exhibit progressive germ cell depletion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Optimized lentiviral vector transduction of adherent cells and analysis in sulforhodamine B proliferation and chromatin immunoprecipitation assays.

STAR protocols

Li L,Trent JC,Eid JE

Fri Mar 17 00:00:00 UTC 2023

Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development.

Nature communications

Gao G,Li X,Jiang Z,Osorio L,Tang YL,Yu X,Jin G,Zhou Z

Tue Apr 25 00:00:00 UTC 2023

Inhibition of MAPK/ERK pathway activation rescues congenital anomalies of the kidney and urinary tract (CAKUT) in Robo2^PB/+ Gen1^PB/+ mice.

Biochemical and biophysical research communications

Du X,Yu M,Ju H,Xue S,Li Y,Wu X,Xu H,Shen Q

Sun Apr 23 00:00:00 UTC 2023

A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility.

Nature communications

Kiyozumi D,Shimada K,Chalick M,Emori C,Kodani M,Oura S,Noda T,Endo T,Matzuk MM,Wreschner DH,Ikawa M

Mon Apr 24 00:00:00 UTC 2023

ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth.

Frontiers in molecular biosciences

Park J,Park GY,Lee J,Park J,Kim S,Kim E,Park SY,Yoon JH,Lee Y

Wed Jan 11 00:00:00 UTC 2023

ETV5 Regulates Hepatic Fatty Acid Metabolism Through PPAR Signaling Pathway.

Diabetes

Mao Z,Feng M,Li Z,Zhou M,Xu L,Pan K,Wang S,Su W,Zhang W

Fri Jan 01 00:00:00 UTC 2021

ETV5 Regulates Hepatic Fatty Acid Metabolism Through PPAR Signaling Pathway.

Diabetes

Mao Z,Feng M,Li Z,Zhou M,Xu L,Pan K,Wang S,Su W,Zhang W

Fri Jan 01 00:00:00 UTC 2021

The dynamic, combinatorial cis-regulatory lexicon of epidermal differentiation.

Nature genetics

Kim DS,Risca VI,Reynolds DL,Chappell J,Rubin AJ,Jung N,Donohue LKH,Lopez-Pajares V,Kathiria A,Shi M,Zhao Z,Deep H,Sharmin M,Rao D,Lin S,Chang HY,Snyder MP,Greenleaf WJ,Kundaje A,Khavari PA

Mon Nov 01 00:00:00 UTC 2021

MAPK pathway activation selectively inhibits ASCL1-driven small cell lung cancer.

iScience

Caeser R,Hulton C,Costa E,Durani V,Little M,Chen X,Tischfield SE,Asher M,Kombak FE,Chavan SS,Shah NS,Ciampricotti M,de Stanchina E,Poirier JT,Rudin CM,Sen T

Fri Nov 19 00:00:00 UTC 2021

Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.

Cell death discovery

Zhou B,Ge Y,Shao Q,Yang L,Chen X,Jiang G

Sat Jun 26 00:00:00 UTC 2021

ETS variant transcription factor 5 and c-Myc cooperate in derepressing the human telomerase gene promoter via composite ETS/E-box motifs.

The Journal of biological chemistry

Zhang F,Wang S,Zhu J

Fri Jul 17 00:00:00 UTC 2020

Capicua restricts cancer stem cell-like properties in breast cancer cells.

Oncogene

Yoe J,Kim D,Kim S,Lee Y

Wed Apr 01 00:00:00 UTC 2020

Regulation of the ERK signalling pathway in the developing mouse blastocyst.

Development (Cambridge, England)

Azami T,Bassalert C,Allègre N,Valverde Estrella L,Pouchin P,Ema M,Chazaud C

Thu Jul 25 00:00:00 UTC 2019

Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription.

Cell

Suh J,Romano DM,Nitschke L,Herrick SP,DiMarzio BA,Dzhala V,Bae JS,Oram MK,Zheng Y,Hooli B,Mullin K,Gennarino VA,Wasco W,Schmahmann JD,Albers MW,Zoghbi HY,Tanzi RE

Thu Aug 22 00:00:00 UTC 2019

FAT4 Fine-Tunes Kidney Development by Regulating RET Signaling.

Developmental cell

Zhang H,Bagherie-Lachidan M,Badouel C,Enderle L,Peidis P,Bremner R,Kuure S,Jain S,McNeill H

Mon Mar 25 00:00:00 UTC 2019

Interaction of BRAF-induced ETS factors with mutant TERT promoter in papillary thyroid cancer.

Endocrine-related cancer

Song YS,Yoo SK,Kim HH,Jung G,Oh AR,Cha JY,Kim SJ,Cho SW,Lee KE,Seo JS,Park YJ

Sat Jun 01 00:00:00 UTC 2019

Capicua regulates neural stem cell proliferation and lineage specification through control of Ets factors.

Nature communications

Ahmad ST,Rogers AD,Chen MJ,Dixit R,Adnani L,Frankiw LS,Lawn SO,Blough MD,Alshehri M,Wu W,Marra MA,Robbins SM,Cairncross JG,Schuurmans C,Chan JA

Wed May 01 00:00:00 UTC 2019

Capicua regulates neural stem cell proliferation and lineage specification through control of Ets factors.

Nature communications

Ahmad ST,Rogers AD,Chen MJ,Dixit R,Adnani L,Frankiw LS,Lawn SO,Blough MD,Alshehri M,Wu W,Marra MA,Robbins SM,Cairncross JG,Schuurmans C,Chan JA

Wed May 01 00:00:00 UTC 2019

ETV5 regulates GOAT/ghrelin system in an mTORC1-dependent manner.

Molecular and cellular endocrinology

Mao Z,Yang Q,Yin W,Su W,Lin H,Feng M,Pan K,Yin Y,Zhang W

Fri Apr 05 00:00:00 UTC 2019

Biological and RNA regulatory function of MOV10 in mammalian germ cells.

BMC biology

Fu K,Tian S,Tan H,Wang C,Wang H,Wang M,Wang Y,Chen Z,Wang Y,Yue Q,Xu Q,Zhang S,Li H,Xie J,Lin M,Luo M,Chen F,Ye L,Zheng K

Tue May 14 00:00:00 UTC 2019

Transcription factor Etv5 is essential for the maintenance of alveolar type II cells.

Proceedings of the National Academy of Sciences of the United States of America

Zhang Z,Newton K,Kummerfeld SK,Webster J,Kirkpatrick DS,Phu L,Eastham-Anderson J,Liu J,Lee WP,Wu J,Li H,Junttila MR,Dixit VM

Tue Apr 11 00:00:00 UTC 2017

Transcription factor Etv5 is essential for the maintenance of alveolar type II cells.

Proceedings of the National Academy of Sciences of the United States of America

Zhang Z,Newton K,Kummerfeld SK,Webster J,Kirkpatrick DS,Phu L,Eastham-Anderson J,Liu J,Lee WP,Wu J,Li H,Junttila MR,Dixit VM

Tue Apr 11 00:00:00 UTC 2017

Transcription factor Etv5 is essential for the maintenance of alveolar type II cells.

Proceedings of the National Academy of Sciences of the United States of America

Zhang Z,Newton K,Kummerfeld SK,Webster J,Kirkpatrick DS,Phu L,Eastham-Anderson J,Liu J,Lee WP,Wu J,Li H,Junttila MR,Dixit VM

Tue Apr 11 00:00:00 UTC 2017

Transcription factor Etv5 is essential for the maintenance of alveolar type II cells.

Proceedings of the National Academy of Sciences of the United States of America

Zhang Z,Newton K,Kummerfeld SK,Webster J,Kirkpatrick DS,Phu L,Eastham-Anderson J,Liu J,Lee WP,Wu J,Li H,Junttila MR,Dixit VM

Tue Apr 11 00:00:00 UTC 2017

Ret and Etv4 Promote Directed Movements of Progenitor Cells during Renal Branching Morphogenesis.

PLoS biology

Riccio P,Cebrian C,Zong H,Hippenmeyer S,Costantini F

Mon Feb 01 00:00:00 UTC 2016

Ret and Etv4 Promote Directed Movements of Progenitor Cells during Renal Branching Morphogenesis.

PLoS biology

Riccio P,Cebrian C,Zong H,Hippenmeyer S,Costantini F

Mon Feb 01 00:00:00 UTC 2016

A critical role for NF2 and the Hippo pathway in branching morphogenesis.

Nature communications

Reginensi A,Enderle L,Gregorieff A,Johnson RL,Wrana JL,McNeill H

Tue Aug 02 00:00:00 UTC 2016

A critical role for NF2 and the Hippo pathway in branching morphogenesis.

Nature communications

Reginensi A,Enderle L,Gregorieff A,Johnson RL,Wrana JL,McNeill H

Tue Aug 02 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: ETS translocation variant 5; ets variant gene 5; ets variant gene 5 (ets-related molecule); ets-related molecule; Ets-related protein ERM

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Gene Aliases: 1110005E01Rik; 8430401F14Rik; ERM; ETV5

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UniProt ID: (Human) P41161, (Mouse) Q9CXC9

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Entrez Gene ID: (Human) 2119, (Rat) 303828, (Mouse) 104156

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription regulatory region DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding molecular_function sequence-specific DNA binding

Process(es)

transcription from RNA polymerase II promoter neuromuscular synaptic transmission locomotory behavior cell differentiation cellular response to oxidative stress positive regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter male germ-line stem cell asymmetric division regulation of synapse organization positive regulation of glial cell proliferation regulation of branching involved in mammary gland duct morphogenesis skeletal muscle acetylcholine-gated channel clustering regulation of transcription from RNA polymerase II promoter positive regulation of transcription, DNA-templated regulation of transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ETV5 Polyclonal Antibody

ETV5 Antibody (13011-1-AP) in ICC/IF

Product Details

13011-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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