Proteintech

EYA3 Polyclonal Antibody

View all (10) EYA3 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (10)

EYA3 Antibody in Immunocytochemistry (ICC/IF)

EYA3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

EYA3 Antibody (21196-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using EYA3 antibody 21196-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

EYA3 Antibody in Immunocytochemistry (ICC/IF)

EYA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EYA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EYA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EYA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EYA3 Antibody in Western Blot (WB)

EYA3 Antibody in Western Blot (WB)

EYA3 Antibody in Western Blot (WB)

EYA3 Antibody in Western Blot (WB)

EYA3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

EYA3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using EYA3 antibody 21196-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

EYA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue slide using (Product # 21196-1-AP) EYA3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EYA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue slide using (Product # 21196-1-AP) EYA3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EYA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21196-1-AP ( EYA3 Antibody) at dilution of 1:50 (under 40x lens).

EYA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21196-1-AP ( EYA3 Antibody) at dilution of 1:50 (under 10x lens).

EYA3 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 21196-1-AP ( EYA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

EYA3 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EYA3 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

EYA3 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

EYA3 Antibody in Immunoprecipitation (IP) — IP result of anti-EYA3 (IP:21196-1-AP, 4ug; Detection:21196-1-AP 1:1000) with L02 cells lysate 1800ug.

Product Details

21196-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

-

View 1 publication 1 publication

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EYA3 Fusion Protein Ag15544 (1-300 aa encoded by BC029500)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to EYA3.

Immunogen sequence: MEEEQDLPE QPVKKAKMQE SGEQTISQVS NPDVSDQKPE TSSLASNLPM SEEIMTCTDY IPRSSNDYTS QMYSAKPYAH ILSVPVSETA YPGQTQYQTL QQTQPYAVYP QATQTYGLPP FGALWPGMKP ESGLIQTPSP SQHSVLTCTT GLTTSQPSPA HYSYPIQASS TNASLISTSS TIANIPAAAV ASISNQDYPT YTILGQNQYQ ACYPSSSFGV TGQTNSDAES TTLAATTYQS EKPSVMAPAP AAQRLSSGDP STSPSLSQTT PSKDTDDQSR KNMTSKNRGK RKADATSSQD S (1-300 aa encoded by BC029500)

Target Information

Eyes absent homolog 3 (EYA3) is a member of the eyes absent (EYA) family of proteins. This protein may act as a transcriptional activator and have a role during development. A similar protein in mice can act as a transcriptional activator. Two transcript variants encoding distinct isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

miR-342-5p downstream to Notch enhances arterialization of endothelial cells in response to shear stress by repressing MYC.

Molecular therapy. Nucleic acids

Zhang X,Sun J,Zhang P,Wen T,Wang R,Liang L,Yang Z,Li J,Zhang J,Che B,Feng X,Liu X,Han H,Yan X

Tue Jun 13 00:00:00 UTC 2023

Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression.

Annals of translational medicine

Yang C,Liu H

Fri Jul 01 00:00:00 UTC 2022

Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression.

Annals of translational medicine

Yang C,Liu H

Fri Jul 01 00:00:00 UTC 2022

Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression.

Annals of translational medicine

Yang C,Liu H

Fri Jul 01 00:00:00 UTC 2022

Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression.

Annals of translational medicine

Yang C,Liu H

Fri Jul 01 00:00:00 UTC 2022

Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression.

Annals of translational medicine

Yang C,Liu H

Fri Jul 01 00:00:00 UTC 2022

Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression.

Annals of translational medicine

Yang C,Liu H

Fri Jul 01 00:00:00 UTC 2022

Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation.

International journal of molecular sciences

Ionescu AE,Mentel M,Munteanu CVA,Sima LE,Martin EC,Necula-Petrareanu G,Szedlacsek SE

Fri Dec 13 00:00:00 UTC 2019

Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation.

International journal of molecular sciences

Ionescu AE,Mentel M,Munteanu CVA,Sima LE,Martin EC,Necula-Petrareanu G,Szedlacsek SE

Fri Dec 13 00:00:00 UTC 2019

The EYA3 tyrosine phosphatase activity promotes pulmonary vascular remodeling in pulmonary arterial hypertension.

Nature communications

Wang Y,Pandey RN,York AJ,Mallela J,Nichols WC,Hu YC,Molkentin JD,Wikenheiser-Brokamp KA,Hegde RS

Thu Sep 12 00:00:00 UTC 2019

The EYA3 tyrosine phosphatase activity promotes pulmonary vascular remodeling in pulmonary arterial hypertension.

Nature communications

Wang Y,Pandey RN,York AJ,Mallela J,Nichols WC,Hu YC,Molkentin JD,Wikenheiser-Brokamp KA,Hegde RS

Thu Sep 12 00:00:00 UTC 2019

The EYA3 tyrosine phosphatase activity promotes pulmonary vascular remodeling in pulmonary arterial hypertension.

Nature communications

Wang Y,Pandey RN,York AJ,Mallela J,Nichols WC,Hu YC,Molkentin JD,Wikenheiser-Brokamp KA,Hegde RS

Thu Sep 12 00:00:00 UTC 2019

WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton.

Scientific reports

Mentel M,Ionescu AE,Puscalau-Girtu I,Helm MS,Badea RA,Rizzoli SO,Szedlacsek SE

Tue Feb 13 00:00:00 UTC 2018

WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton.

Scientific reports

Mentel M,Ionescu AE,Puscalau-Girtu I,Helm MS,Badea RA,Rizzoli SO,Szedlacsek SE

Tue Feb 13 00:00:00 UTC 2018

Structure-activity relationships of benzbromarone metabolites and derivatives as EYA inhibitory anti-angiogenic agents.

PloS one

Pandey RN,Wang TS,Tadjuidje E,McDonald MG,Rettie AE,Hegde RS

Mon Jul 28 00:00:00 UTC 2014

Structure-activity relationships of benzbromarone metabolites and derivatives as EYA inhibitory anti-angiogenic agents.

PloS one

Pandey RN,Wang TS,Tadjuidje E,McDonald MG,Rettie AE,Hegde RS

Mon Jul 28 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: eyes absent 3; Eyes absent homolog 3; Protein phosphatase EYA3

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Gene Aliases: EYA3

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UniProt ID: (Human) Q99504

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Entrez Gene ID: (Human) 2140

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protein tyrosine phosphatase activity protein binding metal ion binding

double-strand break repair transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development visual perception anatomical structure morphogenesis response to ionizing radiation histone dephosphorylation peptidyl-tyrosine dephosphorylation positive regulation of DNA repair

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