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Invitrogen
EZH1 Recombinant Rabbit Monoclonal Antibody (PS01-09)
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FIGURE: 1 / 10
EZH1 Antibody (MA5-46620) in ICC/IF
Immunofluorescence analysis of EZH1 was performed using 70% confluent log phase SK-MEL-5 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Anti-EZH1 Recombinant Rabbit Monoclonal Antibody (PS01-09) (Product # MA5-46620) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alex... View More
Product Details
MA5-46620
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PS01-09
Immunogen
Synthetic peptide within human EZH1 aa 471-510.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937692
Product Specific Information
Positive Control: HCT116 cell lysate, K562 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat large intestine tissue, human colon tissue, mouse large intestine tissue.
Subcellular Location: Nucleus.
Tissue Specificity: Tissue enhanced (retina).
Sequence Similarities: 97% Mouse/Rat.
Target Information
EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Enhancer of zeste homolog 1; ENX-2; Histone-lysine N-methyltransferase EZH1
Gene Aliases: ENX-2; Enx2; EZH1; KIAA0388; KMT6B
UniProt ID: (Human) Q92800, (Mouse) P70351
Entrez Gene ID: (Human) 2145, (Rat) 303547, (Mouse) 14055
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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