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Eg5 Antibody (100-401-J04) in WB
Western Blot of Rabbit Anti-Eg-5 antibody. Lane 1: E.coli cell lysate expressing histidine tagged protein. Lane 2: Mouse brain lysate. Lane 3: Recombinant truncated Eg-5. Lane 4: MW Markers. Load: 35µg/lane for cell lysate, 50ng of recombinant protein. Primary antibody: Eg-5 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 120 kDa and 72 kDa for Eg5.
Product Details
100-401-J04
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Eg5 was prepared from whole rabbit serum produced by repeated immunizations with a truncated Eg5 construct expressed in E. coli corresponding to human Eg5 protein.
Conjugate
Form
Liquid
Concentration
46 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Eg5 is directed against the human Eg5 protein. A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with Eg5 from other sources have not been determined.
Target Information
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Kines; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5
Gene Aliases: EG5; HKSP; KIF11; KNSL1; MCLMR; TRIP5
UniProt ID: (Human) P52732
Entrez Gene ID: (Human) 3832
retrograde vesicle-mediated transport, Golgi to ER
microtubule-based movement
spindle organization
mitotic spindle organization
chromosome segregation
mitotic nuclear division
mitotic centrosome separation
antigen processing and presentation of exogenous peptide antigen via MHC class II
regulation of mitotic centrosome separation
cell division
mitotic spindle assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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