Proteintech

Emerin Polyclonal Antibody

Emerin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

Emerin Antibody (10351-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (Product # 10351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Emerin Antibody in Flow Cytometry (Flow)

Emerin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10351-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 16 publications 16 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Emerin Fusion Protein Ag0236 (66-252 aa encoded by BC000738)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: STRGDADMY DLPKKEDALL YQSKGYNDDY YEESYFTTRT YGEPESAGPS RAVRQSVTSF PDADAFHHQV HDDDLLSSSE EECKDRERPM YGRDSAYQSI THYRPVSASR SSLDLSYYPT SSSTSFMSSS SSSSSWLTRR AIRPENRAPG AGLGQDRQVP LWGQLLLFLV FVIVLFFIYH FMQAEEGN (66-252 aa encoded by BC000738 )

Target Information

Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is an enzyme essential for DNA repair of the major kinds of solar ultraviolet (UV)-induced DNA damages. Human XPG nuclease makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981-1009) contains the PCNA binding activity. A conserved arginine in XPG (Arg992) is crucial for its PCNA binding activity. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, the xeroderma pigmentosum damage-recognition protein XPA and the endonuclease XPG.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chromatin remodeling and nucleoskeleton synergistically control osteogenic differentiation in different matrix stiffnesses.

Materials today. Bio

Xu X,Zhang H,Li Y,Liu F,Jing Z,Ren M,Chen T,Fu Y,Wu Y,Ji P,Yang S

Thu Jun 01 00:00:00 UTC 2023

Chromatin remodeling and nucleoskeleton synergistically control osteogenic differentiation in different matrix stiffnesses.

Materials today. Bio

Xu X,Zhang H,Li Y,Liu F,Jing Z,Ren M,Chen T,Fu Y,Wu Y,Ji P,Yang S

Thu Jun 01 00:00:00 UTC 2023

LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion.

Nature cell biology

Jung-Garcia Y,Maiques O,Monger J,Rodriguez-Hernandez I,Fanshawe B,Domart MC,Renshaw MJ,Marti RM,Matias-Guiu X,Collinson LM,Sanz-Moreno V,Carlton JG

Sun Jan 01 00:00:00 UTC 2023

The BAF A12T mutation disrupts lamin A/C interaction, impairing robust repair of nuclear envelope ruptures in Nestor-Guillermo progeria syndrome cells.

Nucleic acids research

Janssen A,Marcelot A,Breusegem S,Legrand P,Zinn-Justin S,Larrieu D

Fri Sep 09 00:00:00 UTC 2022

The BAF A12T mutation disrupts lamin A/C interaction, impairing robust repair of nuclear envelope ruptures in Nestor-Guillermo progeria syndrome cells.

Nucleic acids research

Janssen A,Marcelot A,Breusegem S,Legrand P,Zinn-Justin S,Larrieu D

Fri Sep 09 00:00:00 UTC 2022

Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.

The Journal of cell biology

Golchoubian B,Brunner A,Bragulat-Teixidor H,Neuner A,Akarlar BA,Ozlu N,Schlaitz AL

Mon Feb 07 00:00:00 UTC 2022

Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery.

Frontiers in cell and developmental biology

Marano N,Holaska JM

Sat Jun 29 00:00:00 UTC 2024

Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer.

World journal of surgical oncology

He T,Zhang Y,Li X,Liu C,Zhu G,Yin X,Zhang Z,Zhao K,Wang Z,Zhao P,Wang K

Thu Jun 02 00:00:00 UTC 2022

Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer.

World journal of surgical oncology

He T,Zhang Y,Li X,Liu C,Zhu G,Yin X,Zhang Z,Zhao K,Wang Z,Zhao P,Wang K

Thu Jun 02 00:00:00 UTC 2022

Recruitment of DNA to tumor-derived microvesicles.

Cell reports

Clancy JW,Sheehan CS,Boomgarden AC,D'Souza-Schorey C

Tue Mar 01 00:00:00 UTC 2022

BCAP31 is involved in modulating colorectal cancer cell proliferation via the Emerin/β-catenin axis.

Experimental cell research

Han L,Shi J,Zhao L,Deng J,Li Y,Zhao H,Wang H,Yan Y,Zou F

Thu Sep 01 00:00:00 UTC 2022

Off-the-shelf proximity biotinylation for interaction proteomics.

Nature communications

Santos-Barriopedro I,van Mierlo G,Vermeulen M

Wed Aug 18 00:00:00 UTC 2021

Off-the-shelf proximity biotinylation for interaction proteomics.

Nature communications

Santos-Barriopedro I,van Mierlo G,Vermeulen M

Wed Aug 18 00:00:00 UTC 2021

Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.

Developmental cell

Merta H,Carrasquillo Rodríguez JW,Anjur-Dietrich MI,Vitale T,Granade ME,Harris TE,Needleman DJ,Bahmanyar S

Mon Dec 20 00:00:00 UTC 2021

Defects in Emerin-Nucleoskeleton Binding Disrupt Nuclear Structure and Promote Breast Cancer Cell Motility and Metastasis.

Molecular cancer research : MCR

Liddane AG,McNamara CA,Campbell MC,Mercier I,Holaska JM

Thu Jul 01 00:00:00 UTC 2021

Defects in Emerin-Nucleoskeleton Binding Disrupt Nuclear Structure and Promote Breast Cancer Cell Motility and Metastasis.

Molecular cancer research : MCR

Liddane AG,McNamara CA,Campbell MC,Mercier I,Holaska JM

Thu Jul 01 00:00:00 UTC 2021

CDK1 controls CHMP7-dependent nuclear envelope reformation.

eLife

Gatta AT,Olmos Y,Stoten CL,Chen Q,Rosenthal PB,Carlton JG

Wed Jul 21 00:00:00 UTC 2021

Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

Nature

Procter DJ,Furey C,Garza-Gongora AG,Kosak ST,Walsh D

Sun Nov 01 00:00:00 UTC 2020

Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

Nature

Procter DJ,Furey C,Garza-Gongora AG,Kosak ST,Walsh D

Sun Nov 01 00:00:00 UTC 2020

EDMD-Causing Emerin Mutant Myogenic Progenitors Exhibit Impaired Differentiation Using Similar Mechanisms.

Cells

Iyer A,Holaska JM

Mon Jun 15 00:00:00 UTC 2020

Emerin Is Required for Proper Nucleus Reassembly after Mitosis: Implications for New Pathogenetic Mechanisms for Laminopathies Detected in EDMD1 Patients.

Cells

Dubińska-Magiera M,Kozioł K,Machowska M,Piekarowicz K,Filipczak D,Rzepecki R

Wed Mar 13 00:00:00 UTC 2019

Emerin Is Required for Proper Nucleus Reassembly after Mitosis: Implications for New Pathogenetic Mechanisms for Laminopathies Detected in EDMD1 Patients.

Cells

Dubińska-Magiera M,Kozioł K,Machowska M,Piekarowicz K,Filipczak D,Rzepecki R

Wed Mar 13 00:00:00 UTC 2019

Targeting of LRRC59 to the Endoplasmic Reticulum and the Inner Nuclear Membrane.

International journal of molecular sciences

Blenski M,Kehlenbach RH

Tue Jan 15 00:00:00 UTC 2019

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 UTC 2018

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 UTC 2018

The effect of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability, and mobility in embryonic cells.

Chromosoma

Piekarowicz K,Machowska M,Dratkiewicz E,Lorek D,Madej-Pilarczyk A,Rzepecki R

Tue Aug 01 00:00:00 UTC 2017

The effect of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability, and mobility in embryonic cells.

Chromosoma

Piekarowicz K,Machowska M,Dratkiewicz E,Lorek D,Madej-Pilarczyk A,Rzepecki R

Tue Aug 01 00:00:00 UTC 2017

The effect of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability, and mobility in embryonic cells.

Chromosoma

Piekarowicz K,Machowska M,Dratkiewicz E,Lorek D,Madej-Pilarczyk A,Rzepecki R

Tue Aug 01 00:00:00 UTC 2017

The effect of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability, and mobility in embryonic cells.

Chromosoma

Piekarowicz K,Machowska M,Dratkiewicz E,Lorek D,Madej-Pilarczyk A,Rzepecki R

Tue Aug 01 00:00:00 UTC 2017

Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression.

BMC cell biology

Smith ER,Meng Y,Moore R,Tse JD,Xu AG,Xu XX

Sat Jan 14 00:00:00 UTC 2017

Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression.

BMC cell biology

Smith ER,Meng Y,Moore R,Tse JD,Xu AG,Xu XX

Sat Jan 14 00:00:00 UTC 2017

DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.

Cell

Samwer M,Schneider MWG,Hoefler R,Schmalhorst PS,Jude JG,Zuber J,Gerlich DW

Thu Aug 24 00:00:00 UTC 2017

Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Journal of cell science

Pfaff J,Rivera Monroy J,Jamieson C,Rajanala K,Vilardi F,Schwappach B,Kehlenbach RH

Mon Feb 01 00:00:00 UTC 2016

Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Journal of cell science

Pfaff J,Rivera Monroy J,Jamieson C,Rajanala K,Vilardi F,Schwappach B,Kehlenbach RH

Mon Feb 01 00:00:00 UTC 2016

Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Journal of cell science

Pfaff J,Rivera Monroy J,Jamieson C,Rajanala K,Vilardi F,Schwappach B,Kehlenbach RH

Mon Feb 01 00:00:00 UTC 2016

Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Journal of cell science

Pfaff J,Rivera Monroy J,Jamieson C,Rajanala K,Vilardi F,Schwappach B,Kehlenbach RH

Mon Feb 01 00:00:00 UTC 2016

Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence.

Carcinogenesis

Lenain C,Gusyatiner O,Douma S,van den Broek B,Peeper DS

Sun Nov 01 00:00:00 UTC 2015

Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence.

Carcinogenesis

Lenain C,Gusyatiner O,Douma S,van den Broek B,Peeper DS

Sun Nov 01 00:00:00 UTC 2015

Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence.

Carcinogenesis

Lenain C,Gusyatiner O,Douma S,van den Broek B,Peeper DS

Sun Nov 01 00:00:00 UTC 2015

Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence.

Carcinogenesis

Lenain C,Gusyatiner O,Douma S,van den Broek B,Peeper DS

Sun Nov 01 00:00:00 UTC 2015

MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.

Scientific reports

Fu Y,Lv P,Yan G,Fan H,Cheng L,Zhang F,Dang Y,Wu H,Wen B

Wed Nov 25 00:00:00 UTC 2015

MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.

Scientific reports

Fu Y,Lv P,Yan G,Fan H,Cheng L,Zhang F,Dang Y,Wu H,Wen B

Wed Nov 25 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Emerin; LEM domain containing 5

View more View less

Gene Aliases: AW550900; EDMD; EMD; LEMD5; STA

View more View less

UniProt ID: (Human) P50402, (Mouse) O08579

View more View less

Entrez Gene ID: (Human) 2010, (Mouse) 13726

View more View less

Function(s)

actin binding protein binding beta-tubulin binding cadherin binding involved in cell-cell adhesion

Process(es)

muscle contraction mitotic nuclear envelope disassembly mitotic nuclear envelope reassembly muscle organ development negative regulation of catenin import into nucleus skeletal muscle cell differentiation positive regulation of protein export from nucleus negative regulation of fibroblast proliferation regulation of canonical Wnt signaling pathway cellular response to growth factor stimulus cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Emerin Polyclonal Antibody

Emerin Antibody (10351-1-AP) in ICC/IF

Product Details

10351-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!