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          • Primary Antibodies ›
          • Endothelin 1 Antibodies

          Invitrogen

          Endothelin 1 Monoclonal Antibody (TR.ET.48.5)

          5 Published Figures
          40 References
          View all (14) Endothelin 1 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite Endothelin 1 Monoclonal Antibody (TR.ET.48.5)

          • Antibody Testing Data (9)
          • Published Figures (5)
          Endothelin 1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Endothelin 1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          Endothelin 1 Antibody (MA3-005) in ICC/IF

          Immunofluorescent analysis of Endothelin 1 (green) showing positive staining in the secretion of HUVEC cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endothelin 1 monoclonal antibody (Product # MA3-005... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Endothelin 1 Antibody in Immunocytochemistry (ICC/IF)
          Endothelin 1 Antibody in Immunocytochemistry (ICC/IF)
          Endothelin 1 Antibody in Immunocytochemistry (ICC/IF)
          Endothelin 1 Antibody in Immunohistochemistry (IHC)
          Endothelin 1 Antibody in Immunohistochemistry (IHC)
          Endothelin 1 Antibody in Western Blot (WB)
          Endothelin 1 Antibody in Flow Cytometry (Flow)
          Endothelin 1 Antibody in Flow Cytometry (Flow)
          Endothelin 1 Antibody in Flow Cytometry (Flow)
          Endothelin 1 Antibody in Immunohistochemistry (IHC)
          Endothelin 1 Antibody in Immunohistochemistry (IHC)
          Endothelin 1 Antibody in Western Blot (WB)
          Endothelin 1 Antibody in Western Blot (WB)
          Endothelin 1 Antibody in Mass Spectrometry (MS)

          Product Details

          MA3-005

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:100-1:1,000
          View 9 publications 9 publications

          Immunohistochemistry (IHC)

          -
          View 27 publications 27 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:250
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          1:250
          -

          Immunocytochemistry (ICC/IF)

          1:200-1:1,000
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          0.5 µg/test
          View 1 publication 1 publication

          Radioimmune Assays (RIA)

          1:25,000
          -

          Mass Spectrometry (MS)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Sheep, Rat

          Published species

          Dog, Human, Mouse, Pig, Rat, Sheep

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          TR.ET.48.5

          Immunogen

          Pure endothelin-1 conjugated to KLH.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          2.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2096246

          Product Specific Information

          MA3-005 detects ET-1 from human, sheep, mouse and rat tissues. This antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides.

          MA3-005 has been successfully used in Western blot, flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and radioimmune assay procedures. Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with MA3-005 results in staining of endothelial cells. Radioimmune assays using MA3-005 can be used to concentrate ET-1 in solution (e.g. serµm/plasma, milk, urine). This antibody is not recommended for human placenta tissue or HepG2 cells in Western blot applications.

          For immunohistochemistry with MA3-005 formalin-fixed, paraffin-embedded tissue sections has been used.

          The MA3-005 antigen is purified human endothelin-1. Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.

          Target Information

          The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Big endothelin 1; Big ET; Big ET 1; Big ET1; BigET; Endothelin-1; Et; ET-1; ppET-1; PPET1; preproendothelin; Preproendothelin-1

          View more View less

          Gene Aliases: ARCND3; EDN1; ET-1; ET1; HDLCQ7; PPET-1; PPET1; preproET; QME

          View more View less

          UniProt ID: (Sheep) Q9BG76, (Human) P05305, (Rat) P22388, (Mouse) P22387

          View more View less

          Entrez Gene ID: (Sheep) 443498, (Human) 1906, (Rat) 24323, (Mouse) 13614

          View more View less

          Function(s)
          cytokine activity hormone activity protein binding endothelin A receptor binding endothelin B receptor binding receptor binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter skeletal system development prostaglandin biosynthetic process patterning of blood vessels response to hypoxia in utero embryonic development histamine secretion regulation of systemic arterial blood pressure by endothelin regulation of pH cell surface receptor signaling pathway G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration protein kinase C-activating G-protein coupled receptor signaling pathway cell-cell signaling heart development respiratory gaseous exchange body fluid secretion regulation of blood pressure positive regulation of cell proliferation dorsal/ventral pattern formation response to ozone multicellular organism aging positive regulation of heart rate positive regulation of cardiac muscle hypertrophy positive regulation of receptor biosynthetic process neural crest cell development phosphatidylinositol 3-kinase signaling response to activity artery smooth muscle contraction vein smooth muscle contraction glucose transport cell growth regulation of vasoconstriction sensory perception of pain calcium-mediated signaling peptide hormone secretion nitric oxide transport positive regulation of cell migration neutrophil chemotaxis negative regulation of cAMP biosynthetic process phospholipase D-activating G-protein coupled receptor signaling pathway negative regulation of cellular protein metabolic process positive regulation of prostaglandin secretion response to lipopolysaccharide response to testosterone negative regulation of smooth muscle cell apoptotic process response to prostaglandin F response to nicotine intracellular signal transduction cellular response to drug positive regulation of urine volume positive regulation of renal sodium excretion response to muscle stretch epithelial fluid transport vasoconstriction protein kinase C deactivation middle ear morphogenesis positive regulation of odontogenesis response to drug superoxide anion generation rhythmic excitation response to amino acid positive regulation of MAP kinase activity positive regulation of JUN kinase activity response to leptin positive regulation of cell size positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle contraction positive regulation of hormone secretion negative regulation of hormone secretion inositol phosphate-mediated signaling blood vessel morphogenesis positive regulation of smooth muscle cell proliferation cartilage development positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway negative regulation of nitric-oxide synthase biosynthetic process membrane depolarization regulation of sensory perception of pain maternal process involved in parturition positive regulation of sarcomere organization positive regulation of prostaglandin-endoperoxide synthase activity cellular response to calcium ion cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to peptide hormone stimulus cellular response to glucocorticoid stimulus cellular response to mineralocorticoid stimulus cellular response to fatty acid cellular response to hypoxia response to dexamethasone response to transforming growth factor beta cellular response to transforming growth factor beta stimulus positive regulation of neutrophil chemotaxis response to salt regulation of blood vessel size positive regulation of endothelial cell migration negative regulation of blood coagulation leukocyte activation positive regulation of nitric oxide biosynthetic process positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of cell growth involved in cardiac muscle cell development positive regulation of chemokine-mediated signaling pathway
          It has to be done as per old AB suggested Products section.
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