Invitrogen

EphB4 Monoclonal Antibody (3D7G8)

EphB4 Antibody in Immunocytochemistry (ICC/IF)

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EphB4 Antibody (37-1800) in ICC/IF

Immunofluorescent analysis of EphB4 (green) in HT29 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a EphB4 mouse monoclonal antibody (Product # 37-1800) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731) at a dilution of 1:500 for at least 30 minutes at a room ... View More

EphB4 Antibody in Immunohistochemistry (IHC)

EphB4 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin), Flow Cytometry (ICC/IF, IHC (P), Flow)

EphB4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

EphB4 Antibody — Figure 5 .Immunohistochemistry was used to detect ephrin-B and EphB4 protein expression. Representative immunostaining of a (A) negative ephrin-B tumor, (B) membranous ephrin-B, (C) EphB4-negative, (D) nuclear EphB4 and (E) membranous EphB4. Scale bars, 100 u m. (F) EphB4 antibody validation in MCF7 cells was performed by immunoblotting and the intensity of the bands quantified (n=2). Error bars represent the standard error of the mean (SEM). The AllStar siRNA was used as negative control and GAPDH siRNA as positive control. The ephrin B antibody was the same used in all the in vitro experiments. {KD}

Product Details

37-1800

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

3-5 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Peptide Array (Array)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3D7G8

Immunogen

Recombinant protein derived from the C-terminal region of the human EphB4 protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533303

Product Specific Information

37-1800 has been successfully used in Western Blot, immunohistochemical, immunofluorescence, ELISA and immunoprecipitation assays. By western blot this antibody detects a ~125kDa protein representing EPHB4 in MCF7, HT-29 and U2OS lysate.

Target Information

EPHB4 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluation of EphB4 as Target for Image-Guided Surgery of Breast Cancer.

Pharmaceuticals (Basel, Switzerland)

de Muijnck C,van Gorkom Y,van Duijvenvoorde M,Eghtesadi M,Dekker-Ensink G,Bhairosingh SS,Affinito A,Kuppen PJK,Vahrmeijer AL,Sier CFM

37-1800 was used in Immunohistochemistry to prove that EphB4 is a promising candidate for targeted image-guided surgery of breast cancer, especially for Her2 negative cases.

Thu Jul 30 00:00:00 UTC 2020

Evaluation of EphB4 as Target for Image-Guided Surgery of Breast Cancer.

Pharmaceuticals (Basel, Switzerland)

de Muijnck C,van Gorkom Y,van Duijvenvoorde M,Eghtesadi M,Dekker-Ensink G,Bhairosingh SS,Affinito A,Kuppen PJK,Vahrmeijer AL,Sier CFM

37-1800 was used in Immunohistochemistry to prove that EphB4 is a promising candidate for targeted image-guided surgery of breast cancer, especially for Her2 negative cases.

Thu Jul 30 00:00:00 UTC 2020

Human blood vessel organoids as a model of diabetic vasculopathy.

Nature

Wimmer RA,Leopoldi A,Aichinger M,Wick N,Hantusch B,Novatchkova M,Taubenschmid J,Hämmerle M,Esk C,Bagley JA,Lindenhofer D,Chen G,Boehm M,Agu CA,Yang F,Fu B,Zuber J,Knoblich JA,Kerjaschki D,Penninger JM

37-1800 was used in Immunocytochemistry-immunoflourescence to suggest that blood vessel organoids derived from human stem cells faithfully recapitulate the structure and function of human blood vessels and are a suitable model for diabetic vasculopathy.

Tue Jan 01 00:00:00 UTC 2019

Ephrin‑B2 inhibits cell proliferation and motility in vitro and predicts longer metastasis‑free survival in breast cancer.

International journal of oncology

Magic Z,Sandström J,Perez-Tenorio G

Published figure using EphB4 monoclonal antibody (Product # 37-1800) in Western Blot

Sun Dec 01 00:00:00 UTC 2019

Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery.

International journal of molecular sciences

Stammes MA,Prevoo HA,Ter Horst MC,Groot SA,Van de Velde CJ,Chan AB,de Geus-Oei LF,Kuppen PJ,Vahrmeijer AL,Pasquale EB,Sier CF

371800 was used in immunohistochemistry - paraffin section to examine if EphA2 and EphB4 proteins are suitable targets for image-guided oncologic surgery of colorectal cancer

Fri Feb 03 00:00:00 UTC 2017

Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery.

International journal of molecular sciences

Stammes MA,Prevoo HA,Ter Horst MC,Groot SA,Van de Velde CJ,Chan AB,de Geus-Oei LF,Kuppen PJ,Vahrmeijer AL,Pasquale EB,Sier CF

371800 was used in immunohistochemistry - paraffin section to examine if EphA2 and EphB4 proteins are suitable targets for image-guided oncologic surgery of colorectal cancer

Fri Feb 03 00:00:00 UTC 2017

Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery.

International journal of molecular sciences

Stammes MA,Prevoo HA,Ter Horst MC,Groot SA,Van de Velde CJ,Chan AB,de Geus-Oei LF,Kuppen PJ,Vahrmeijer AL,Pasquale EB,Sier CF

371800 was used in immunohistochemistry - paraffin section to examine if EphA2 and EphB4 proteins are suitable targets for image-guided oncologic surgery of colorectal cancer

Fri Feb 03 00:00:00 UTC 2017

Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery.

International journal of molecular sciences

Stammes MA,Prevoo HA,Ter Horst MC,Groot SA,Van de Velde CJ,Chan AB,de Geus-Oei LF,Kuppen PJ,Vahrmeijer AL,Pasquale EB,Sier CF

371800 was used in immunohistochemistry - paraffin section to examine if EphA2 and EphB4 proteins are suitable targets for image-guided oncologic surgery of colorectal cancer

Fri Feb 03 00:00:00 UTC 2017

Erythropoietin Stimulates Tumor Growth via EphB4.

Cancer cell

Pradeep S,Huang J,Mora EM,Nick AM,Cho MS,Wu SY,Noh K,Pecot CV,Rupaimoole R,Stein MA,Brock S,Wen Y,Xiong C,Gharpure K,Hansen JM,Nagaraja AS,Previs RA,Vivas-Mejia P,Han HD,Hu W,Mangala LS,Zand B,Stagg LJ,Ladbury JE,Ozpolat B,Alpay SN,Nishimura M,Stone RL,Matsuo K,Armaiz-Peña GN,Dalton HJ,Danes C,Goodman B,Rodriguez-Aguayo C,Kruger C,Schneider A,Haghpeykar S,Jaladurgam P,Hung MC,Coleman RL,Liu J,Li C,Urbauer D,Lopez-Berestein G,Jackson DB,Sood AK

Published figure using EphB4 monoclonal antibody (Product # 37-1800) in Immunohistochemistry

Mon Nov 09 00:00:00 UTC 2015

YES oncogenic activity is specified by its SH4 domain and regulates RAS/MAPK signaling in colon carcinoma cells.

American journal of cancer research

Dubois F,Leroy C,Simon V,Benistant C,Roche S

37-1800 was used in immunoprecipitation to elucidate how YES contributes to colorectal cancer progression

Thu Aug 13 00:00:00 UTC 2015

Gene expression profiling identifies EPHB4 as a potential predictive biomarker in colorectal cancer patients treated with bevacizumab.

Medical oncology (Northwood, London, England)

Guijarro-Muñoz I,Sánchez A,Martínez-Martínez E,García JM,Salas C,Provencio M,Alvarez-Vallina L,Sanz L

37-1800 was used in immunohistochemistry (paraffin) to investigate the prognostic value of EPHB4 in colorectal cancer therapy.

Fri Oct 25 00:00:00 UTC 2013

EPHB4 and survival of colorectal cancer patients.

Cancer research

Davalos V,Dopeso H,Castaño J,Wilson AJ,Vilardell F,Romero-Gimenez J,Espín E,Armengol M,Capella G,Mariadason JM,Aaltonen LA,Schwartz S,Arango D

37-1800 was used in immunohistochemistry - paraffin section and western blot to study the role of EPHB4 in colorectal cancer and determine its value as a prognostic marker

Fri Sep 15 00:00:00 UTC 2006

EphB receptor-binding peptides identified by phage display enable design of an antagonist with ephrin-like affinity.

The Journal of biological chemistry

Koolpe M,Burgess R,Dail M,Pasquale EB

Fri Apr 29 00:00:00 UTC 2005

EphB receptor-binding peptides identified by phage display enable design of an antagonist with ephrin-like affinity.

The Journal of biological chemistry

Koolpe M,Burgess R,Dail M,Pasquale EB

Fri Apr 29 00:00:00 UTC 2005

EphB receptor-binding peptides identified by phage display enable design of an antagonist with ephrin-like affinity.

The Journal of biological chemistry

Koolpe M,Burgess R,Dail M,Pasquale EB

Fri Apr 29 00:00:00 UTC 2005

EphB receptor-binding peptides identified by phage display enable design of an antagonist with ephrin-like affinity.

The Journal of biological chemistry

Koolpe M,Burgess R,Dail M,Pasquale EB

Fri Apr 29 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11

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Gene Aliases: EPHB4; HTK; MYK1; TYRO11

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UniProt ID: (Human) P54760

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Entrez Gene ID: (Human) 2050

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Function(s)

transmembrane receptor protein tyrosine kinase activity ephrin receptor activity protein binding ATP binding

Process(es)

angiogenesis cell migration involved in sprouting angiogenesis heart morphogenesis cell adhesion peptidyl-tyrosine phosphorylation protein autophosphorylation ephrin receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EphB4 Monoclonal Antibody (3D7G8)

EphB4 Antibody (37-1800) in ICC/IF

Product Details

37-1800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Peptide Array (Array)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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