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.jpg?time=20250104 "Epha1 Antibody in Immunohistochemistry (Frozen) (IHC (F))")
FIGURE: 1 / 2
Epha1 Antibody (PA5-47435) in IHC (F)
Immunohistochemical analysis of EphA1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-mouse EphA1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47435) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue). Specific labeling was localized to thymic stromal cells.
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.jpg?time=20250104?time=20250104 "Epha1 Antibody (PA5-47435) in IHC (F)")
Product Details
PA5-47435
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphA1 Glu27-Glu548
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607409
Product Specific Information
In direct ELISAs, approximately 35% cross-reactivity with recombinant human EphA1 and less than 5% cross-reactivity with recombinant mouse (rm) EphA4, rmEphA6, and rmEphA7 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
EphA1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA1 is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Embryonic stem cell kinase; Ephrin type-A receptor 1; mEpha1; MGC163163; Tyrosine-protein kinase receptor ESK
Gene Aliases: 5730453L17Rik; AL033318; Eph; Epha1; Esk
UniProt ID: (Mouse) Q60750
Entrez Gene ID: (Mouse) 13835
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
ephrin receptor activity
transmembrane-ephrin receptor activity
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
angiogenesis
positive regulation of cell-matrix adhesion
protein phosphorylation
negative regulation of protein kinase activity
cell adhesion
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
phosphorylation
peptidyl-tyrosine phosphorylation
positive regulation of cell migration
negative regulation of cell migration
substrate adhesion-dependent cell spreading
regulation of GTPase activity
positive regulation of angiogenesis
protein autophosphorylation
ephrin receptor signaling pathway
positive regulation of stress fiber assembly
activation of GTPase activity
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