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Invitrogen
Ephrin A5 Polyclonal Antibody
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.jpg?time=20250104 "Ephrin A5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))")
FIGURE: 1 / 1
Ephrin A5 Antibody (PA5-47532) in IHC (P)
Immunohistochemical analysis of Ephrin-A5 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-human Ephrin-A5 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47532) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.
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.jpg?time=20250104?time=20250104 "Ephrin A5 Antibody (PA5-47532) in IHC (P)")
Product Details
PA5-47532
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ephrin-A5 Gln21-Asn203
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608568
Product Specific Information
In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-A1, rmEphrin-A2, recombinant human (rh) Ephrin-A3, rhEphrin-A4, rhEphrin-B3, rmEphrin-A4, rmEphrin-B1 and rmEphrin-B2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Ephrin A5 is a cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AL-1; EPH-related receptor tyrosine kinase ligand 7; Ephrin-A5; LERK-7
Gene Aliases: AF1; EFL5; EFNA5; EPLG7; GLC1M; LERK7; RAGS
UniProt ID: (Human) P52803
Entrez Gene ID: (Human) 1946
positive regulation of protein phosphorylation
apoptotic process
activation of transmembrane receptor protein tyrosine kinase activity
nervous system development
axon guidance
regulation of cell-cell adhesion
regulation of cell morphogenesis
retinal ganglion cell axon guidance
regulation of actin cytoskeleton organization
regulation of GTPase activity
ephrin receptor signaling pathway
collateral sprouting
positive regulation of collateral sprouting
positive regulation of peptidyl-tyrosine phosphorylation
negative chemotaxis
regulation of focal adhesion assembly
positive regulation of synapse assembly
regulation of insulin secretion involved in cellular response to glucose stimulus
regulation of microtubule cytoskeleton organization
negative regulation of substrate adhesion-dependent cell spreading
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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