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Invitrogen
Ephrin B1 Monoclonal Antibody (2D3E9)
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FIGURE: 1 / 7
Ephrin B1 Antibody (37-8100) in ICC/IF
Immunofluorescent analysis of Ephrin-B Pan was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ephrin-B Pan (2D3E9) Mouse Monoclonal Antibody (Product # 37-8100) at 2 µg/mL in 0.1% BSA and incubated for ... View More
Product Details
37-8100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2D3E9
Immunogen
Synthetic peptide derived from the ephrin-B sequence.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533342
Target Information
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CEK5 ligand; CEK5-L; CEL5-L; EFL-3; EFL6; EFNB3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; Ephrin B3; Ephrin-B1; Ephrin-B1 precursor; EphrinB1; EPLG8; kinase ephrin-B1; LERK-2; LERK8; STRA1 protein
Gene Aliases: CFND; CFNS; EFB1; EFL3; EFNB1; Elk-L; EPLG2; LERK2
UniProt ID: (Chicken) O73612, (Human) P98172
Entrez Gene ID: (Chicken) 395896, (Human) 1947
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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