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Invitrogen
Ephrin B2 Recombinant Rabbit Monoclonal Antibody (JM53-21)
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FIGURE: 1 / 8
Ephrin B2 Antibody (MA5-32740) in ICC/IF
Immunofluorescence analysis of EFNB2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Ephrin B2 Recombinant Rabbit Monoclonal Antibody (JM53-21) (Product # MA5-32740) at 1 µg/mL in 0.1% BSA, incubated at... View More
Product Details
MA5-32740
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM53-21
Immunogen
Synthetic peptide within Human Ephrin B aa 47-96
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2810017
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ELF-2; EPH-related receptor tyrosine kinase ligand 5; Ephrin-B2; HTK ligand; HTK-L; LERK-5; ligand of eph-related kinase 5; MGC126226; MGC126227; MGC126228
Gene Aliases: EFNB2; ELF-2; Elf2; Epl5; EPLG5; Htk-L; HTKL; LERK-5; LERK5; NLERK-1
UniProt ID: (Human) P52799, (Mouse) P52800
Entrez Gene ID: (Human) 1948, (Mouse) 13642, (Rat) 306636
angiogenesis
lymph vessel development
cell migration involved in sprouting angiogenesis
cell adhesion
multicellular organism development
nervous system development
axon guidance
organ morphogenesis
negative regulation of keratinocyte proliferation
cell migration
cell differentiation
ephrin receptor signaling pathway
blood vessel morphogenesis
regulation of chemotaxis
nephric duct morphogenesis
positive regulation of cardiac muscle cell differentiation
cell-cell signaling
anatomical structure morphogenesis
T cell costimulation
viral entry into host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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