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Invitrogen
Epiregulin Polyclonal Antibody
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Epiregulin Antibody (PA5-46969) in IHC (P)
Immunohistochemical analysis of Epiregulin in immersion fixed paraffin-embedded sections of human skin. Samples were incubated in Epiregulin polyclonal antibody (Product # PA5-46969) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes.
Product Details
PA5-46969
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human Epiregulin Val63-Leu108
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610406
Product Specific Information
In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Epiregulin is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Epiregulin is an EGF-related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes, as well as in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0 kDa mature secreted sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Proepiregulin
Gene Aliases: Ep; EPR; ER; EREG
UniProt ID: (Human) O14944
Entrez Gene ID: (Human) 2069
MAPK cascade
angiogenesis
ovarian cumulus expansion
oocyte maturation
positive regulation of cytokine production
female meiotic division
epidermal growth factor receptor signaling pathway
cell-cell signaling
positive regulation of cell proliferation
negative regulation of cell proliferation
mRNA transcription
anatomical structure morphogenesis
organ morphogenesis
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
keratinocyte differentiation
ovulation
ERBB2 signaling pathway
wound healing
positive regulation of cytokine biosynthetic process
positive regulation of phosphorylation
luteinizing hormone signaling pathway
response to peptide hormone
positive regulation of GTPase activity
keratinocyte proliferation
positive regulation of innate immune response
positive regulation of interleukin-6 biosynthetic process
positive regulation of DNA replication
positive regulation of epidermal growth factor-activated receptor activity
positive regulation of mitotic nuclear division
positive regulation of protein kinase activity
negative regulation of transcription, DNA-templated
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of fibroblast proliferation
primary follicle stage
positive regulation of smooth muscle cell proliferation
negative regulation of epithelial cell proliferation
negative regulation of smooth muscle cell differentiation
positive regulation of cell division
regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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