Invitrogen

ErbB2 (HER-2) Monoclonal Antibody (MJD2), eBioscience™

ErbB2 (HER-2) Antibody in Immunocytochemistry (ICC/IF)

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ErbB2 (HER-2) Antibody (14-9757-95) in ICC/IF

Immunocytochemistry of fixed and permeabilized BT474 cells using 5 µg/mL of Anti-Human Epidermal Growth Factor Receptor-2 (ErbB2/HER-2) Purified followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.

ErbB2 (HER-2) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ErbB2 (HER-2) Antibody in Western Blot (WB)

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Product Details

14-9757-95

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MJD2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_2865418

Product Specific Information

Description: This MJD2 monoclonal antibody reacts with HER-2, the product of the ErbB2 gene and a member of the human epidermal growth factor receptor (EGFR) family. HER-2 is a 185 kDa transmembrane receptor with an extracellular binding domain and an intracellular tyrosine kinase domain. Activation of HER-2 occurs following formation of homodimers or heterodimers with ligand-bound EGFR family members, HER3 or HER4. Overexpression of HER-2 results in auto-phosphorylation and increased proliferation and invasiveness and is associated with subsets of multiple cancer types such as breast, ovarian, cervical, uterine, and gastric. The use of HER-2 targeted agents has been used extensively to treat HER-2-positive breast cancer, but a recent study found that these agents can also block the proliferation of breast cancer stem cells found in HER-2-negative patients and suggests the importance of HER-2 as a target for multiple types of breast cancer. The MJD2 antibody recognizes the extracellular domain of HER-2 but at an epitope distinct from that recognized by the 4D5 (Herceptin) and SP3 monoclonal antibodies.

Applications Reported: This MJD2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This MJD2 antibody has been tested by immunhistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This MJD2 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized human BT474 cells and can be used at less than or equal to 5 µg/mL. This MJD2 antibody has been tested by western blot analysis of reduced human BT474 cell lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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