Invitrogen

ErbB3 Monoclonal Antibody (RTJ2)

Advanced Verification
3 References
View all (82) Her-3 (ErbB3) antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-860

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
View 2 publications 2 publications

Flow Cytometry (Flow)

0.5-2 µg / 10^6 cells
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RTJ2

Immunogen

Synthetic Peptide: E(1004) L E P E L D L D L D L E(1016)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325378

Product Specific Information

MA1-860 detects Her-3 from human and mouse samples.

MA1-860 has been successfully used in FACS, immunoprecipitation, immunohistochemical, immunofluorescence and ELISA procedures. By immunoprecipitation, this antibody detects ~185 kDa protein, representing Her-3 from human tissue extracts.This antibody has also been used in immunohistochemical, immunofluorescence and ELISA procedures using human tissues.

MA1-860 immunizing peptide corresponds to amino acid residues 1004 to 1016 of human cytoplasmic domain of HER-3 protein.

Target Information

ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family. ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6. 2kb mRNA which is translated into a 160/180 kDa glycoprotein. The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity. Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation. Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized. One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: avian erythroblastosis oncogene B 3 receptor; c-erbB-3; C-errB-3; Glial growth factor receptor; human epidermal growth factor receptor 3; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine kinase; Receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3

View more View less

Gene Aliases: c-erbB-3; c-erbB3; C76256; ErbB-3; ERBB3; erbB3-S; Erbb3r; HER3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3

View more View less

UniProt ID: (Human) P21860, (Mouse) Q61526

View more View less

Entrez Gene ID: (Human) 2065, (Mouse) 13867

View more View less

Function(s)
protein tyrosine kinase activity transmembrane signaling receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding growth factor binding protein tyrosine kinase activator activity neuregulin binding identical protein binding protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity nucleotide binding protein kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups neuregulin receptor activity
Process(es)
endocardial cushion development protein phosphorylation negative regulation of cell adhesion signal transduction transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway peripheral nervous system development negative regulation of signal transduction Schwann cell differentiation phosphatidylinositol 3-kinase signaling phosphorylation cranial nerve development regulation of cell proliferation negative regulation of neuron apoptotic process positive regulation of glucose import negative regulation of secretion neuron apoptotic process positive regulation of protein tyrosine kinase activity positive regulation of calcineurin-NFAT signaling cascade extrinsic apoptotic signaling pathway in absence of ligand MAPK cascade heart development regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation ERBB2 signaling pathway wound healing positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of cell motility
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support