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Invitrogen
ErbB3 (HER-3) Monoclonal Antibody (SGP1), PerCP-eFluor™ 710, eBioscience™
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ErbB3 (HER-3) Antibody (46-6555-42) in Flow
Knockout of ErbB3 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR764358_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of ErbB3 was performed by staining T-47D ErbB3 Knock out cells with0.125 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ (Product # 46-4714-82, yellow histogram) or0.125 µgErbB3 (HER-3) Monoclonal Antibody (SGP1), PerCP-eFluor™ 710, eBioscience™ (Product # 46-6555-42, blue histogram).T47D Cas9 ... View More
Product Details
46-6555-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
SGP1
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10853823
Product Specific Information
Description: This SGP1 monoclonal antibody reacts with human c-ErbB3, which is also known as HER3. This epidermal growth factor (EGF) receptor family member is expressed on normal human tissues, particularly on neurons and cells of the gastrointestinal tract. c-ErbB3 is overexpressed in a variety of cancers, including breast, ovary, and gastrointestinal carcinomas. Upon binding its ligand heregulin beta-1, c-ErbB3 is activated by heterodimerization with ErbB2 or EGFR1. Dependent on PI3K signaling, c-ErbB3 mediates tumor cell invasion, intravasation, and metastasis. Expression of this cell surface receptor has been used as a prognostic marker for breast cancer.
The SGP1 antibody has been reported to stimulate anchorage-independent growth of breast cancer cell lines.
Applications Reported: This SGP1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SGP1 antibody has been pre-titrated and tested by flow cytometric analysis of the MCF7 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family. ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6. 2kb mRNA which is translated into a 160/180 kDa glycoprotein. The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity. Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation. Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized. One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: C-errB-3; human epidermal growth factor receptor 3; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; Proto-oncogene-like protein c-ErbB-3; Receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
Gene Aliases: c-erbB-3; c-erbB3; ErbB-3; ERBB3; erbB3-S; HER3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3
UniProt ID: (Human) P21860
Entrez Gene ID: (Human) 2065
protein tyrosine kinase activity
transmembrane signaling receptor activity
Ras guanyl-nucleotide exchange factor activity
protein binding
ATP binding
growth factor binding
protein tyrosine kinase activator activity
neuregulin binding
identical protein binding
protein homodimerization activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
protein heterodimerization activity
MAPK cascade
endocardial cushion development
negative regulation of cell adhesion
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
peripheral nervous system development
heart development
negative regulation of signal transduction
Schwann cell differentiation
phosphatidylinositol 3-kinase signaling
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cranial nerve development
ERBB2 signaling pathway
wound healing
regulation of cell proliferation
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
negative regulation of secretion
neuron apoptotic process
positive regulation of protein tyrosine kinase activity
positive regulation of calcineurin-NFAT signaling cascade
extrinsic apoptotic signaling pathway in absence of ligand
regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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