Invitrogen

ErbB4 Monoclonal Antibody (HFR1)

1 Published Figure

34 References

View all (49) ErbB4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (13)
Published Figures (1)

ErbB4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

ErbB4 Antibody (MA1-861) in ICC/IF

Immunofluorescent analysis of ErbB4 (green) showing staining in the cytoplasm of SK-BR-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an ErbB4 monoclonal antibody (Product # MA1-861) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjug... View More

ErbB4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ErbB4 Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-861

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 28 publications 28 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

1:50

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5-2 µg / test

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

HFR1

Immunogen

Synthetic Peptide: C R(1250) S T L Q H P D Y L Q E Y S T(1264)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325379

Product Specific Information

MA1-861 detects Her-4 from human and mouse samples.

MA1-861 has been successfully used in immunoprecipitation, immunohistochemical, and Western blotting procedures. By Western blot, this antibody detects ~185 kDa protein representing Her-4 from human A431 cell and mouse NIH3T3/HER4 cell extracts.This antibody has also been used in immunohistochemical, using paraffin embedded mouse NIH3T3 cells.

MA1-861 immunizing peptide corresponds to amino acid residues 1250 to 1264 of human Her-4 oncoprotein.

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nrg1β Released in Remote Ischemic Preconditioning Improves Myocardial Perfusion and Decreases Ischemia/Reperfusion Injury via ErbB2-Mediated Rescue of Endothelial Nitric Oxide Synthase and Abrogation of Trx2 Autophagy.

Arteriosclerosis, thrombosis, and vascular biology

Kundumani-Sridharan V,Subramani J,Owens C,Das KC

MA1-861 was used in Proximity Ligation Assay (PLA) to conclude that Nrg1β is a RIPC factor that interacts with endothelial ErbB2 and prevents its degradation, which in turn prevents Trx2 degradation due to phosphorylation and inactivation of ATG5 (autophagy-related 5) by ErbB2.

Sun Aug 01 00:00:00 UTC 2021

Screening for potential targets to reduce stenosis in bioprosthetic heart valves.

Scientific reports

Foth R,Shomroni O,Sigler M,Hörer J,Cleuziou J,Paul T,Eildermann K

MA1-861 was used in Immunohistochemistry to suggest EGFR, ErbB4 and FLT4 as potential target candidates to limit pannus formation in bioprosthestic replacement valves.

Thu Jan 28 00:00:00 UTC 2021

Screening for potential targets to reduce stenosis in bioprosthetic heart valves.

Scientific reports

Foth R,Shomroni O,Sigler M,Hörer J,Cleuziou J,Paul T,Eildermann K

MA1-861 was used in Immunohistochemistry to suggest EGFR, ErbB4 and FLT4 as potential target candidates to limit pannus formation in bioprosthestic replacement valves.

Thu Jan 28 00:00:00 UTC 2021

EV20‑sss‑vc/MMAF, an HER‑3 targeting antibody‑drug conjugate displays antitumor activity in liver cancer.

Oncology reports

D'Agostino D,Gentile R,Ponziani S,Di Vittorio G,Dituri F,Giannelli G,Rossi C,Marzullo L,Giansanti F,De Laurenzi V,Iacobelli S,Ippoliti R,Capone E,Sala G

MA1-861 was used in Flow cytometry/Cell sorting to indicate that EV20‚sss‚vc/MMAF is a worthy candidate for the treatment of HER‚3 positive LC.

Mon Feb 01 00:00:00 UTC 2021

The reemergence of long-term potentiation in aged Alzheimer's disease mouse model.

Scientific reports

Huh S,Baek SJ,Lee KH,Whitcomb DJ,Jo J,Choi SM,Kim DH,Park MS,Lee KH,Kim BC

MA1861 was used in western blot to investigate the reemergence of hippocampal long-term potential in a mouse model of Alzheimer's disease

Tue Jul 05 00:00:00 UTC 2016

Prognostic and biological significance of proliferation and HER2 expression in the luminal class of breast cancer.

Breast cancer research and treatment

Jerjees DA,Alabdullah M,Green AR,Alshareeda A,Macmillan RD,Ellis IO,Rakha EA

MA1-861 was used in immunohistochemistry to study the prognostic and clinical value of Ki67 proliferation index and HER2 expression in luminal breast cancer

Sun Jun 01 00:00:00 UTC 2014

Expression of the epidermal growth factor system in human middle ear cholesteatoma.

Acta oto-laryngologica

Thorup MB,Munk M,Poulsen SS,Gaihede M,Nexo E,Sorensen BS,Ovesen T

MA1-861 was used in immunohistochemistry to study the expression of the EGFR system in patients with middle ear cholesteatoma

Sat Feb 01 00:00:00 UTC 2014

CYT-1 isoform of ErbB4 is an independent prognostic factor in serous ovarian cancer and selectively promotes ovarian cancer cell growth in vitro.

Gynecologic oncology

Paatero I,Lassus H,Junttila TT,Kaskinen M,Bützow R,Elenius K

MA1-861 was used in immunohistochemistry to study the role and prognostic value of ErbB4 CYT-1 isoform expression in serous ovarian cancer

Mon Apr 01 00:00:00 UTC 2013

Cystatin M loss is associated with the losses of estrogen receptor, progesterone receptor, and HER4 in invasive breast cancer.

Breast cancer research : BCR

Ko E,Park SE,Cho EY,Kim Y,Hwang JA,Lee YS,Nam SJ,Bang S,Park J,Kim DH

MA1-861 was used in immunohistochemistry to study the clinicopathological significance of cystatin M loss in invasive breast cancer

Mon Sep 12 00:00:00 UTC 2011

Overexpression of c-erbB2 is a negative prognostic factor in anaplastic astrocytomas.

Diagnostic pathology

Gulati S,Ytterhus B,Granli US,Gulati M,Lydersen S,Torp SH

MA1-861 was used in immunohistochemistry to study the prognostic value of c-erbB2 expression in anaplastic astrocytomas

Tue Mar 23 00:00:00 UTC 2010

HER-family gene amplification and expression in resected pancreatic cancer.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology

te Velde EA,Franke AC,van Hillegersberg R,Elshof SM,de Weger RW,Borel Rinkes IH,van Diest PJ

MA1-861 was used in immunohistochemistry to study the immunohistochemical expression of HER1-4 and gene amplification of HER2 in resected pancreatic cancer

Thu Oct 01 00:00:00 UTC 2009

The HER4/4ICD estrogen receptor coactivator and BH3-only protein is an effector of tamoxifen-induced apoptosis.

Cancer research

Naresh A,Thor AD,Edgerton SM,Torkko KC,Kumar R,Jones FE

MA1-861 was used in immunohistochemistry to study the role of HER4/4ICD and BH3-only protein in tamoxifen-induced apoptosis

Fri Aug 01 00:00:00 UTC 2008

Distinctive expression pattern of ErbB family receptors signifies an aggressive variant of bladder cancer.

The Journal of urology

Kassouf W,Black PC,Tuziak T,Bondaruk J,Lee S,Brown GA,Adam L,Wei C,Baggerly K,Bar-Eli M,McConkey D,Czerniak B,Dinney CP

MA1-861 was used in immunohistochemistry to investigate the association of expression of various members of ErbB family receptors with progression of urothelial carcinoma

Tue Jan 01 00:00:00 UTC 2008

Expression of tumor necrosis factor alpha converting enzyme in endocrine cancers.

American journal of clinical pathology

Kirkegaard T,Naresh A,Sabine VS,Tovey SM,Edwards J,Dunne B,Cooke TG,Jones FE,Bartlett JM

MA1-861 was used in immunohistochemistry to study the immunohistochemical expression of TACE in endocrine cancers and its potential role in breast cancer apoptosis

Thu May 01 00:00:00 UTC 2008

Phosphorylated HER-2 tyrosine kinase and Her-2/neu gene amplification as predictive factors of response to trastuzumab in patients with HER-2 overexpressing metastatic breast cancer (MBC).

European journal of cancer (Oxford, England : 1990)

Giuliani R,Durbecq V,Di Leo A,Paesmans M,Larsimont D,Leroy JY,Borms M,Vindevoghel A,Jerusalem G,D'Hondt V,Dirix L,Canon JL,Richard V,Cocquyt V,Majois F,Reginster M,Demol J,Kains JP,Delree P,Keppens C,Sotiriou C,Piccart MJ,Cardoso F

MA1-861 was used in immunohistochemistry to identify prognostic indicators of response/resistance to trastuzumab in metastatic breast cancer

Thu Mar 01 00:00:00 UTC 2007

Differential nuclear localization and kinase activity of alternative ErbB4 intracellular domains.

Oncogene

Sundvall M,Peri L,Määttä JA,Tvorogov D,Paatero I,Savisalo M,Silvennoinen O,Yarden Y,Elenius K

MA1-861 was used in immunocytochemistry to study the localization and function of alternative ErbB4 intracellular domains

Thu Oct 18 00:00:00 UTC 2007

Expression of the epidermal growth factor system in endometrioid endometrial cancer.

Gynecologic oncology

Ejskjaer K,Sørensen BS,Poulsen SS,Forman A,Nexø E,Mogensen O

MA1-861 was used in immunohistochemistry to study the expression of the EGF system in endometrioid endometrial cancer

Mon Jan 01 00:00:00 UTC 2007

Coexpression of c-erbB 1-4 receptor proteins in human glioblastomas. An immunohistochemical study.

Journal of experimental & clinical cancer research : CR

Torp SH,Gulati S,Johannessen E,Dalen A

MA1-861 was used in immunohistochemistry to study the coexpression of c-erbB 1-4 receptor proteins in human glioblastomas

Sat Sep 01 00:00:00 UTC 2007

The role of HER1-HER4 and EGFRvIII in hormone-refractory prostate cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Edwards J,Traynor P,Munro AF,Pirret CF,Dunne B,Bartlett JM

MA1-861 was used in immunohistochemistry to study the role of HER1-HER4 and EGFRvIII in hormone-refractory prostate cancer

Sun Jan 01 00:00:00 UTC 2006

Morphological and immunophenotypic analysis of breast carcinomas with basal and myoepithelial differentiation.

The Journal of pathology

Rakha EA,Putti TC,Abd El-Rehim DM,Paish C,Green AR,Powe DG,Lee AH,Robertson JF,Ellis IO

MA1-861 was used in immunohistochemistry to study the morphological and immunophenotypic features of breast carcinomas with basal and myoepithelial differentiation

Wed Mar 01 00:00:00 UTC 2006

Ovarian granulosa cell tumors frequently express EGFR (Her-1), Her-3, and Her-4: An immunohistochemical study.

Gynecologic oncology

Leibl S,Bodo K,Gogg-Kammerer M,Hrzenjak A,Petru E,Winter R,Denk H,Moinfar F

MA1-861 was used in immunohistochemistry to study the immunohistochemical expression of EGFR family members in ovarian granulosa cell tumors

Sat Apr 01 00:00:00 UTC 2006

Essential erbB family phosphorylation in osteosarcoma as a target for CI-1033 inhibition.

Pediatric blood & cancer

Hughes DP,Thomas DG,Giordano TJ,McDonagh KT,Baker LH

MA1-861 was used in immunohistochemistry to study the role of erbB family phosphorylation in osteosarcoma

Mon May 01 00:00:00 UTC 2006

Prognostic significance of HER3 and HER4 protein expression in colorectal adenocarcinomas.

BMC cancer

Kountourakis P,Pavlakis K,Psyrri A,Rontogianni D,Xiros N,Patsouris E,Pectasides D,Economopoulos T

MA1-861 was used in immunohistochemistry to study the prognostic value of HER3 and HER4 expression in colorectal adenocarcinomas

Tue Feb 28 00:00:00 UTC 2006

NRG1 gene rearrangements in clinical breast cancer: identification of an adjacent novel amplicon associated with poor prognosis.

Oncogene

Prentice LM,Shadeo A,Lestou VS,Miller MA,deLeeuw RJ,Makretsov N,Turbin D,Brown LA,Macpherson N,Yorida E,Cheang MC,Bentley J,Chia S,Nielsen TO,Gilks CB,Lam W,Huntsman DG

MA1-861 was used in immunohistochemistry to investigate the association of NRG1 gene rearrangements with poor prognosis in breast cancer treatment

Thu Nov 10 00:00:00 UTC 2005

High-throughput protein expression analysis using tissue microarray technology of a large well-characterised series identifies biologically distinct classes of breast cancer confirming recent cDNA expression analyses.

International journal of cancer

Abd El-Rehim DM,Ball G,Pinder SE,Rakha E,Paish C,Robertson JF,Macmillan D,Blamey RW,Ellis IO

MA1-861 was used in immunohistochemistry to discuss revising the classification systems for breast cancer

Thu Sep 01 00:00:00 UTC 2005

Coexpression of the type 1 growth factor receptor family members HER-1, HER-2, and HER-3 has a synergistic negative prognostic effect on breast carcinoma survival.

Cancer

Wiseman SM,Makretsov N,Nielsen TO,Gilks B,Yorida E,Cheang M,Turbin D,Gelmon K,Huntsman DG

MA1-861 was used in immunohistochemistry to study the frequency and prognostic value of coexpression of HER-1, HER-2, HER-3, and HER-4 by breast carcinoma

Sun May 01 00:00:00 UTC 2005

H-RAS V12-induced radioresistance in HCT116 colon carcinoma cells is heregulin dependent.

Molecular cancer therapeutics

Carón RW,Yacoub A,Zhu X,Mitchell C,Han SI,Sasazuki T,Shirasawa S,Hagan MP,Grant S,Dent P

MA1-861 was used in western blot to investigate the mechanism for radioresistance induced by H-RAS V12 in HCT116 cells

Tue Feb 01 00:00:00 UTC 2005

Correlation between human epidermal growth factor receptor family (EGFR, HER2, HER3, HER4), phosphorylated Akt (P-Akt), and clinical outcomes after radiation therapy in carcinoma of the cervix.

Gynecologic oncology

Lee CM,Shrieve DC,Zempolich KA,Lee RJ,Hammond E,Handrahan DL,Gaffney DK

MA1-861 was used in immunohistochemistry to study the prognostic value of EGFR family member expression and phospo-Akt levels in cervical carcinoma treated with radiation therapy

Tue Nov 01 00:00:00 UTC 2005

Paclitaxel and carboplatin as first-line chemotherapy combined with gefitinib (IRESSA) in patients with advanced breast cancer: a phase I/II study conducted by the Hellenic Cooperative Oncology Group.

Breast cancer research and treatment

Fountzilas G,Pectasides D,Kalogera-Fountzila A,Skarlos D,Kalofonos HP,Papadimitriou C,Bafaloukos D,Lambropoulos S,Papadopoulos S,Kourea H,Markopoulos C,Linardou H,Mavroudis D,Briasoulis E,Pavlidis N,Razis E,Kosmidis P,Gogas H

MA1-861 was used in immunohistochemistry to investigate the efficacy of the combination of paclitaxel, carboplatin and gefitinib against advanced breast cancer

Fri Jul 01 00:00:00 UTC 2005

Expression of the epidermal growth factor system in human endometrium during the menstrual cycle.

Molecular human reproduction

Ejskjaer K,Sørensen BS,Poulsen SS,Mogensen O,Forman A,Nexø E

MA1-861 was used in immunohistochemistry to study the importance of the EGF system in the growth of the human endometrium

Mon Aug 01 00:00:00 UTC 2005

Expression and co-expression of the members of the epidermal growth factor receptor (EGFR) family in invasive breast carcinoma.

British journal of cancer

Abd El-Rehim DM,Pinder SE,Paish CE,Bell JA,Rampaul RS,Blamey RW,Robertson JF,Nicholson RI,Ellis IO

MA1-861 was used in immunohistochemistry to study the expression of EGFR family members in invasive breast cancers

Mon Oct 18 00:00:00 UTC 2004

Exquisite antitumour response to trastuzumab in a patient with no evidence of Ras-Raf-MAPK and PI3K-Akt pathways activation.

Breast (Edinburgh, Scotland)

Mano MS,Awada A,Minisini A,Durbecq V,Di Leo A,Piccart M

MA1-861 was used in immunohistochemistry to report on a case of a patient with HER2-overexpressing metastatic breast cancer highly sensitive to trastuzumab treatment

Sun Aug 01 00:00:00 UTC 2004

A recurrent chromosome translocation breakpoint in breast and pancreatic cancer cell lines targets the neuregulin/NRG1 gene.

Genes, chromosomes & cancer

Adélaïde J,Huang HE,Murati A,Alsop AE,Orsetti B,Mozziconacci MJ,Popovici C,Ginestier C,Letessier A,Basset C,Courtay-Cahen C,Jacquemier J,Theillet C,Birnbaum D,Edwards PA,Chaffanet M

MA1-861 was used in immunohistochemistry to investigate a specific chromosomal aberration in breast and pancreatic cancer cell lines

Fri Aug 01 00:00:00 UTC 2003

CD44 anchors the assembly of matrilysin/MMP-7 with heparin-binding epidermal growth factor precursor and ErbB4 and regulates female reproductive organ remodeling.

Genes & development

Yu WH,Woessner JF,McNeish JD,Stamenkovic I

MA1-861 was used in immunohistochemistry and western blot to study the role of CD44 in forming a HSPG/MMP7/HB-EGF/ErbB4 complex and its role in regulating female reproductive organ remodelling

Fri Feb 01 00:00:00 UTC 2002

Radiosensitization of malignant glioma cells through overexpression of dominant-negative epidermal growth factor receptor.

Clinical cancer research : an official journal of the American Association for Cancer Research

Lammering G,Valerie K,Lin PS,Mikkelsen RB,Contessa JN,Feden JP,Farnsworth J,Dent P,Schmidt-Ullrich RK

MA1-861 was used in western blot to study the role of a dominant-negative EGFR in the radiosensitization of malignant glioma cells

Thu Mar 01 00:00:00 UTC 2001

Bioinformatics

Protein Aliases: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

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Gene Aliases: ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4

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UniProt ID: (Human) Q15303, (Mouse) Q61527

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Entrez Gene ID: (Human) 2066, (Mouse) 13869

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Function(s)

protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding protein binding ATP binding protein homodimerization activity transcription regulatory region DNA binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ErbB4 Monoclonal Antibody (HFR1)

ErbB4 Antibody (MA1-861) in ICC/IF

Product Details

MA1-861

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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