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          • Primary Antibodies ›
          • Estrogen Receptor alpha Antibodies
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          Invitrogen

          Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11)

          Advanced Verification
          3 Published Figures
          64 References
          View all (168) Estrogen Receptor alpha antibodies

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          Datasheet
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          Datasheet
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          Cite Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11)

          • Antibody Testing Data (5)
          • Published Figures (3)
          • Advanced Verification (1)
          Estrogen Receptor alpha Antibody in Western Blot (WB)
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          Estrogen Receptor alpha Antibody in Western Blot (WB)
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          FIGURE: 1 / 9

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          Estrogen Receptor alpha Antibody (MA5-13065) in WB

          Western blot analysis of Estrogen Receptor (ER) was performed by loading the indicated amounts of recombinant full-length human ER alpha (Product # A15674) and ER beta (Product # A15664) proteins, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Blotter ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Estrogen Receptor alpha Antibody in Western Blot (WB)
          Estrogen Receptor alpha Antibody in Western Blot (WB)
          Estrogen Receptor alpha Antibody in Western Blot (WB)
          Estrogen Receptor alpha Antibody in Immunoprecipitation (IP)
          Estrogen Receptor alpha Antibody in Immunoprecipitation (IP)
          Estrogen Receptor alpha Antibody in Immunoprecipitation (IP)
          Estrogen Receptor alpha Antibody in ChIP Assay (ChIP)
          Estrogen Receptor alpha Antibody in ChIP Assay (ChIP)
          Estrogen Receptor alpha Antibody

          Product Details

          MA5-13065

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200-1:1,000
          View 14 publications 14 publications

          Immunohistochemistry (IHC)

          -
          View 5 publications 5 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          2 µg/mL
          View 7 publications 7 publications

          ChIP assay (ChIP)

          Assay-dependent
          View 23 publications 23 publications

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          Gel Shift (GS)

          1mg/mL
          View 4 publications 4 publications

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications
          Product Specifications

          Published species

          Bovine, Chicken, Human, Mouse, Pig, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          TE111.5D11

          Immunogen

          35kDa, C-terminal fragment (aa 302-595) of human ER expressed in E. coli

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Storage conditions

          4°C

          Shipping conditions

          Wet ice

          RRID

          AB_10979037

          Product Specific Information

          MA5-13065 targets Estrogen Receptor in ChIP, GS, IP, and WB applications and shows reactivity with Bovine, Canine, Chicken, Hamster, Human, mouse, Ovine, Porcine, Rabbit, and Rat samples. The TE111.5D11 clone was developed against the entire ER ligand binding domain (LBD), but has greatest affinity for ER-alpha. Because ER-alpha and ER-beta share 50-60% homology, MA5-13065 recognizes ER-beta1 with lower affinity.

          The MA5-13065 immunogen is 35kDa, C-terminal fragment (aa 302-595) of human ER expressed in E. coli.

          Target Information

          Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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