Invitrogen

Estrogen Receptor beta Polyclonal Antibody

Estrogen Receptor beta Antibody in Immunocytochemistry (ICC/IF)

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Estrogen Receptor beta Antibody (PA1-313) in ICC/IF

Immunofluorescence analysis of Estrogen Receptor beta was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Estrogen Receptor beta Polyclonal Antibody (Product # PA1-313) at 1:100 dilution in 0.1% BSA, incub... View More

Estrogen Receptor beta Antibody in Immunohistochemistry (IHC)

Estrogen Receptor beta Antibody in Western Blot (WB)

Estrogen Receptor beta Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Estrogen Receptor beta Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Estrogen Receptor beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Estrogen Receptor beta Antibody in Western Blot, Gel Shift (WB, GS)

Estrogen Receptor beta Antibody in Gel Shift (GS)

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Product Details

PA1-313

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

2-5 µg/mL

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Gel Shift (GS)

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2102375

Product Specific Information

PA1-313 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing ER beta from rat brain homogenate. Immunohistochemical staining of ER beta in rat prostate with PA1-313 results in nuclear staining, whereas nuclear staining may not be observed in human tissue.

The PA1-313 immunogen is a synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta. PA1-313 immunizing peptide (Cat. # PEP-029) is available for use in neutralization and control experiments.

Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.

Target Information

Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The absence of oestrogen receptor beta disturbs collagen I type deposition during Achilles tendon healing by regulating the IRF5-CCL3 axis.

Journal of cellular and molecular medicine

Bian X,Liu T,Yang M,Gu C,He G,Zhou M,Tang H,Lu K,Lai F,Wang F,Yang Q,Gustafsson JÅ,Fan X,Tang K

PA1-313 was used in Immunohistochemistry to reveal that ERβ regulates Col I deposition during ATH via the IRF5-CCL3 axis.

Tue Sep 01 00:00:00 UTC 2020

The absence of oestrogen receptor beta disturbs collagen I type deposition during Achilles tendon healing by regulating the IRF5-CCL3 axis.

Journal of cellular and molecular medicine

Bian X,Liu T,Yang M,Gu C,He G,Zhou M,Tang H,Lu K,Lai F,Wang F,Yang Q,Gustafsson JÅ,Fan X,Tang K

PA1-313 was used in Immunohistochemistry to reveal that ERβ regulates Col I deposition during ATH via the IRF5-CCL3 axis.

Tue Sep 01 00:00:00 UTC 2020

The absence of oestrogen receptor beta disturbs collagen I type deposition during Achilles tendon healing by regulating the IRF5-CCL3 axis.

Journal of cellular and molecular medicine

Bian X,Liu T,Yang M,Gu C,He G,Zhou M,Tang H,Lu K,Lai F,Wang F,Yang Q,Gustafsson JÅ,Fan X,Tang K

PA1-313 was used in Immunohistochemistry to reveal that ERβ regulates Col I deposition during ATH via the IRF5-CCL3 axis.

Tue Sep 01 00:00:00 UTC 2020

Interaction Proteomics Identifies ERbeta Association with Chromatin Repressive Complexes to Inhibit Cholesterol Biosynthesis and Exert An Oncosuppressive Role in Triple-negative Breast Cancer.

Molecular & cellular proteomics : MCP

Alexandrova E,Giurato G,Saggese P,Pecoraro G,Lamberti J,Ravo M,Rizzo F,Rocco D,Tarallo R,Nyman TA,Collina F,Cantile M,Di Bonito M,Botti G,Nassa G,Weisz A

PA1-313 was used in Immunoprecipitation to explore the potential of targeting Estrogen Receptor beta (ERβ) and its role in triple-negative breast cancer cells.

Sat Feb 01 00:00:00 UTC 2020

Insights into the Role of Estrogen Receptor β in Triple-Negative Breast Cancer.

Cancers

Sellitto A,D'Agostino Y,Alexandrova E,Lamberti J,Pecoraro G,Memoli D,Rocco D,Coviello E,Giurato G,Nassa G,Tarallo R,Weisz A,Rizzo F

PA1-313 was used in Western Blot, Immunohistochemistry to comprehensively review the current state of knowledge concerning the role of estrogen receptor β in triple-negative breast cancer biology.

Fri Jun 05 00:00:00 UTC 2020

Insights into the Role of Estrogen Receptor β in Triple-Negative Breast Cancer.

Cancers

Sellitto A,D'Agostino Y,Alexandrova E,Lamberti J,Pecoraro G,Memoli D,Rocco D,Coviello E,Giurato G,Nassa G,Tarallo R,Weisz A,Rizzo F

PA1-313 was used in Western Blot, Immunohistochemistry to comprehensively review the current state of knowledge concerning the role of estrogen receptor β in triple-negative breast cancer biology.

Fri Jun 05 00:00:00 UTC 2020

Absence of estrogen receptor beta leads to abnormal adipogenesis during early tendon healing by an up-regulation of PPARγ signalling.

Journal of cellular and molecular medicine

Bian X,Liu T,Zhou M,He G,Ma Y,Shi Y,Wang Y,Tang H,Kang X,Yang M,Gustafsson JÅ,Fan X,Tang K

Published figure using Estrogen Receptor beta polyclonal antibody (Product # PA1-313) in Immunofluorescence

Fri Nov 01 00:00:00 UTC 2019

Effect of estradiol on fibroblasts from postmenopausal idiopathic carpal tunnel syndrome patients.

Journal of cellular physiology

Yamanaka Y,Menuki K,Tajima T,Okada Y,Kosugi K,Zenke Y,Sakai A

PA1-313 was used in Immunocytochemistry and Immunohistochemistry to indicate that ERα-mediated signaling may be involved in the regulation of Col1A1, and its regulatory effect may be dependent on the ERα expression level.

Thu Nov 01 00:00:00 UTC 2018

Effect of estradiol on fibroblasts from postmenopausal idiopathic carpal tunnel syndrome patients.

Journal of cellular physiology

Yamanaka Y,Menuki K,Tajima T,Okada Y,Kosugi K,Zenke Y,Sakai A

Thu Nov 01 00:00:00 UTC 2018

Targeting Estrogen Receptor Beta in a Phase 2 Study of High-Dose Estradiol in Metastatic Triple-Negative Breast Cancer: A Wisconsin Oncology Network Study.

Clinical breast cancer

Wisinski KB,Xu W,Tevaarwerk AJ,Saha S,Kim K,Traynor A,Dietrich L,Hegeman R,Patel D,Blank J,Harter J,Burkard ME

PA1-313 was used in immunohistochemistry - paraffin section to analyze a Wisconsin oncology network study of metastatic triple-negative breast cancer where targeting estrogen receptor beta in a phase 2 study of high-dose estradiol

Mon Aug 01 00:00:00 UTC 2016

Targeting Estrogen Receptor Beta in a Phase 2 Study of High-Dose Estradiol in Metastatic Triple-Negative Breast Cancer: A Wisconsin Oncology Network Study.

Clinical breast cancer

Wisinski KB,Xu W,Tevaarwerk AJ,Saha S,Kim K,Traynor A,Dietrich L,Hegeman R,Patel D,Blank J,Harter J,Burkard ME

Mon Aug 01 00:00:00 UTC 2016

Prognostic significance of full-length estrogen receptor beta expression in stage I-III triple negative breast cancer.

American journal of translational research

Shanle EK,Onitilo AA,Huang W,Kim K,Zang C,Engel JM,Xu W,Wisinski KB

PA1-313 was used in immunohistochemistry and western blot to examine the association between ERbeta1 and Ki67 expression in non-metastatic triple negative breast cancer

Wed Sep 02 00:00:00 UTC 2015

Prognostic significance of full-length estrogen receptor beta expression in stage I-III triple negative breast cancer.

American journal of translational research

Shanle EK,Onitilo AA,Huang W,Kim K,Zang C,Engel JM,Xu W,Wisinski KB

PA1-313 was used in immunohistochemistry and western blot to examine the association between ERbeta1 and Ki67 expression in non-metastatic triple negative breast cancer

Wed Sep 02 00:00:00 UTC 2015

Histone H3 Lysine 27 demethylases Jmjd3 and Utx are required for T-cell differentiation.

Nature communications

Manna S,Kim JK,Baugé C,Cam M,Zhao Y,Shetty J,Vacchio MS,Castro E,Tran B,Tessarollo L,Bosselut R

PA1-313 was used in immunohistochemistry and western blot to test if H3K27Me3 demethylation affects transcription and cell differentiation

Wed Sep 02 00:00:00 UTC 2015

Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.

Clinical biochemistry

Kruer TL,Cummins TD,Powell DW,Wittliff JL

PA1-313 was used in EMSA to study the prognostic value of estrogen resonse element binding proteins in breast cancer

Fri Nov 01 00:00:00 UTC 2013

Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors.

Cancer cell international

Yusuf R,Frenkel K

Published figure using Estrogen Receptor beta polyclonal antibody (Product # PA1-313) in Western Blot

Wed Sep 01 00:00:00 UTC 2010

Prepubertal physical activity up-regulates estrogen receptor beta, BRCA1 and p53 mRNA expression in the rat mammary gland.

Breast cancer research and treatment

Wang M,Yu B,Westerlind K,Strange R,Khan G,Patil D,Boeneman K,Hilakivi-Clarke L

PA1-313 was used in immunohistochemistry to study the effects of physical activity on the expression of the tumor suppressor genes BRCA1, p53 and estrogen receptor-beta in prepupertal rats and the significance for breast cancer

Fri May 01 00:00:00 UTC 2009

Estrogen increases survival in an orthotopic model of glioblastoma.

Journal of neuro-oncology

Barone TA,Gorski JW,Greenberg SJ,Plunkett RJ

PA1-313 was used in western blot to investigate the effect of estrogen on glioblastoma

Thu Oct 01 00:00:00 UTC 2009

Differing transcriptional responses to pulsed or continuous estradiol exposure in human umbilical vein endothelial cells.

The Journal of steroid biochemistry and molecular biology

Li J,Wang H,Johnson SM,Horner-Glister E,Thompson J,White IN,Al-Azzawi F

PA1-313 was used in western blot to study the effect of estradiol on transcriptional responses in human umbilical vein endothelial cells

Tue Jul 01 00:00:00 UTC 2008

Changes in biomarkers of estrogen receptor and growth factor signaling pathways in MCF-7 tumors after short- and long-term treatment with soy and flaxseed.

The Journal of steroid biochemistry and molecular biology

Power KA,Chen JM,Saarinen NM,Thompson LU

PA1-313 was used in immunohistochemistry to study the effects of soy and flaxseed on tumor growth and estrogen receptor and growth factor signaling pathways in human breast cancer cells using a murine xenograft model

Sat Nov 01 00:00:00 UTC 2008

Changes in transcription profile and cytoskeleton morphology in pelvic ligament fibroblasts in response to stretch: the effects of estradiol and levormeloxifene.

Molecular human reproduction

Ewies AA,Elshafie M,Li J,Stanley A,Thompson J,Styles J,White I,Al-Azzawi F

PA1-313 was used in western blot to investigate the effect of mechanical stretch on gene transcription and cytoskeletonal organization in pelvic ligament fibroblasts

Fri Feb 01 00:00:00 UTC 2008

Estrogen receptor beta displays cell cycle-dependent expression and regulates the G1 phase through a non-genomic mechanism in prostate carcinoma cells.

Cellular oncology : the official journal of the International Society for Cellular Oncology

Hurtado A,Pinós T,Barbosa-Desongles A,López-Avilés S,Barquinero J,Petriz J,Santamaria-Martínez A,Morote J,de Torres I,Bellmunt J,Reventós J,Munell F

PA1-313 was used in Western Blotting to demonstrate that, in LNCaP prostate cancer cells, both ERbeta isoforms are differentially expressed during the cell cycle and that ERbeta regulates the G1 phase by a non-genomic mechanism.

Fri Oct 10 00:00:00 UTC 2008

Nuclear estrogen receptor beta in lung cancer: expression and survival differences by sex.

Clinical cancer research : an official journal of the American Association for Cancer Research

Schwartz AG,Prysak GM,Murphy V,Lonardo F,Pass H,Schwartz J,Brooks S

PA1-313 was used in immunohistochemistry to demonstrate the sex-related expression of nuclear estrogen receptor beta in lung cancer.

Sat Oct 15 00:00:00 UTC 2005

Estrogen receptors in the uterus and ovarian follicles of gilts treated with dihydrotestosterone.

Domestic animal endocrinology

Cárdenas H,Pope WF

PA1-313 was used in immunohistochemistry to investigate the expression of estrogen receptor alpha and beta in the uterus and ovarian follicles

Sat Oct 01 00:00:00 UTC 2005

Binding of estrogen receptor beta to estrogen response element in situ is independent of estradiol and impaired by its amino terminus.

Molecular endocrinology (Baltimore, Md.)

Huang J,Li X,Maguire CA,Hilf R,Bambara RA,Muyan M

PA1-313 was used in immunohistochemistry to study the contribution of distinct amino termini of estrogen receptors towards their DNA response element binding and subsequent transcription efficacies.

Tue Nov 01 00:00:00 UTC 2005

Oestrogen receptor beta: what it means for patients with breast cancer.

The Lancet. Oncology

Speirs V,Carder PJ,Lane S,Dodwell D,Lansdown MR,Hanby AM

PA1-313 was used in immunohistochemistry to review the significance of oestrogen receptor-beta in breast cancer patients

Mon Mar 01 00:00:00 UTC 2004

Single-chain estrogen receptors (ERs) reveal that the ERalpha/beta heterodimer emulates functions of the ERalpha dimer in genomic estrogen signaling pathways.

Molecular and cellular biology

Li X,Huang J,Yi P,Bambara RA,Hilf R,Muyan M

PA1-313 was used in western blot to study the function of estrogen receptor alpha and beta heterodimer.

Wed Sep 01 00:00:00 UTC 2004

Single-chain estrogen receptors (ERs) reveal that the ERalpha/beta heterodimer emulates functions of the ERalpha dimer in genomic estrogen signaling pathways.

Molecular and cellular biology

Li X,Huang J,Yi P,Bambara RA,Hilf R,Muyan M

PA1-313 was used in western blot to study the function of estrogen receptor alpha and beta heterodimer.

Wed Sep 01 00:00:00 UTC 2004

Single-chain estrogen receptors (ERs) reveal that the ERalpha/beta heterodimer emulates functions of the ERalpha dimer in genomic estrogen signaling pathways.

Molecular and cellular biology

Li X,Huang J,Yi P,Bambara RA,Hilf R,Muyan M

PA1-313 was used in western blot to study the function of estrogen receptor alpha and beta heterodimer.

Wed Sep 01 00:00:00 UTC 2004

Single-chain estrogen receptors (ERs) reveal that the ERalpha/beta heterodimer emulates functions of the ERalpha dimer in genomic estrogen signaling pathways.

Molecular and cellular biology

Li X,Huang J,Yi P,Bambara RA,Hilf R,Muyan M

PA1-313 was used in western blot to study the function of estrogen receptor alpha and beta heterodimer.

Wed Sep 01 00:00:00 UTC 2004

Estrogen regulates adrenal angiotensin AT1 receptors by modulating AT1 receptor translation.

Endocrinology

Wu Z,Maric C,Roesch DM,Zheng W,Verbalis JG,Sandberg K

PA1-313 was used in immunohistochemistry to investigate the molecular mechanisms for estrgen regulation of AT1R gene expression in the rat adrenal gland.

Tue Jul 01 00:00:00 UTC 2003

17Beta-estradiol up-regulates vascular endothelial growth factor receptor-2 expression in human myometrial microvascular endothelial cells: role of estrogen receptor-alpha and -beta.

The Journal of clinical endocrinology and metabolism

Gargett CE,Zaitseva M,Bucak K,Chu S,Fuller PJ,Rogers PA

PA1-313 was used in western blot to investigate the role of estrogen receptor-alpha and -beta in human myometrial microvascular endothelial cells.

Sun Sep 01 00:00:00 UTC 2002

17-Beta-estradiol regulates expression of genes that function in macrophage activation and cholesterol homeostasis.

The Journal of steroid biochemistry and molecular biology

Kramer PR,Wray S

PA1-313 was used in western blot to investigate the regulation of 17-beta-estradiol on the expression of genes functioning in macrophage activation and cholesterol metabolism

Mon Jul 01 00:00:00 UTC 2002

Loss of estrogen receptor beta expression in malignant human prostate cells in primary cultures and in prostate cancer tissues.

The Journal of clinical endocrinology and metabolism

Pasquali D,Rossi V,Esposito D,Abbondanza C,Puca GA,Bellastella A,Sinisi AA

PA1-313 was used in western blot to investigate the expression of ER beta and alpha in malignant human prostate cells

Tue May 01 00:00:00 UTC 2001

Different regulation of oestrogen receptors alpha and beta in the human cervix at term pregnancy.

Molecular human reproduction

Wang H,Stjernholm Y,Ekman G,Eriksson H,Sahlin L

PA1-313 was used in immunohistochemistry to study the expression change of the two ERs in the cervix from women at term pregnancy and immediately post-partum and to try to find its relationship with cervical ripening

Thu Mar 01 00:00:00 UTC 2001

Estrogen receptors in the normal adult and developing human inner ear and in Turner's syndrome.

Hearing research

Stenberg AE,Wang H,Fish J,Schrott-Fischer A,Sahlin L,Hultcrantz M

PA1-313 was used in immunohistochemistry to investigate the expression and activity of estrogen receptors in human sensory tissues

Sun Jul 01 00:00:00 UTC 2001

Estrogen receptor beta is coexpressed with ERalpha and PR and associated with nodal status, grade, and proliferation rate in breast cancer.

The American journal of pathology

Järvinen TA,Pelto-Huikko M,Holli K,Isola J

PA1-313 was used in immunohistochemistry the study the expression of estrogen receptor beta in normal and pathological human tissues.

Sat Jan 01 00:00:00 UTC 2000

Estrogen receptor beta is coexpressed with ERalpha and PR and associated with nodal status, grade, and proliferation rate in breast cancer.

The American journal of pathology

Järvinen TA,Pelto-Huikko M,Holli K,Isola J

PA1-313 was used in immunohistochemistry the study the expression of estrogen receptor beta in normal and pathological human tissues.

Sat Jan 01 00:00:00 UTC 2000

Identification and developmental expression of the estrogen receptor alpha and beta in the baboon fetal adrenal gland.

Endocrinology

Albrecht ED,Babischkin JS,Davies WA,Leavitt MG,Pepe GJ

PA1-313 was used in immunohistochemistry to study the expression of the estrogen receptor alpha and beta in the adrenal gland

Wed Dec 01 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: ER; ER beta; ER-beta; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta

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Gene Aliases: ER-BETA; ER[b]; Erb; Erb2; ERbeta; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2

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UniProt ID: (Human) Q92731, (Mouse) O08537, (Rat) Q62986

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Entrez Gene ID: (Human) 2100, (Mouse) 13983, (Rat) 25149

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Function(s)

core promoter sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding steroid binding protein binding drug binding zinc ion binding enzyme binding estrogen receptor activity estrogen response element binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding peroxisome proliferator activated receptor binding receptor antagonist activity estradiol binding steroid hormone binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding lipid binding hormone binding sequence-specific DNA binding metal ion binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Estrogen Receptor beta Polyclonal Antibody

Estrogen Receptor beta Antibody (PA1-313) in ICC/IF

Product Details

PA1-313

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Gel Shift (GS)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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