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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Ezrin Antibodies
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          Invitrogen

          Ezrin Monoclonal Antibody (3C12)

          Advanced Verification
          8 Published Figures
          61 References
          View all (37) Ezrin antibodies

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          Datasheet
          Protocols
          Datasheet
          Protocols

          Cite Ezrin Monoclonal Antibody (3C12)

          • Antibody Testing Data (12)
          • Published Figures (8)
          • Advanced Verification (2)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
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          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 22

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          Ezrin Antibody (MA5-13862) in ICC/IF

          Immunofluorescent analysis of Phalloidin (magenta) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Ezrin Antibody in Western Blot (WB)
          Ezrin Antibody in Immunoprecipitation (IP)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Ezrin Antibody in Immunoprecipitation (IP)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Ezrin Antibody in Western Blot (WB)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunocytochemistry (ICC/IF)
          Ezrin Antibody in Immunohistochemistry (IHC)
          Ezrin Antibody in Proximity Ligation Assay (PLA) (PLA)
          Ezrin Antibody in Proximity Ligation Assay (PLA) (PLA)
          Ezrin Antibody
          Ezrin Antibody

          Product Details

          MA5-13862

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5-1.0 µg/mL
          View 15 publications 15 publications

          Immunohistochemistry (IHC)

          -
          View 24 publications 24 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1-2 µg/mL
          View 7 publications 7 publications

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          View 8 publications 8 publications

          Immunoprecipitation (IP)

          2 µg/mL
          View 1 publication 1 publication

          in situ PLA (PLA)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 5 publications 5 publications
          Product Specifications

          Published species

          Dog, Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3C12

          Immunogen

          A recombinant fragment of human ezrin corresponding to aa 362-585
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10979020

          Product Specific Information

          MA5-13862 targets Ezrin in IHC (P), ICC, IP, IF, and WB applications and shows reactivity with Bovine, Hamster, Human, Marsupial, mouse, Non-human primate, and Rat samples.

          The MA5-13862 immunogen is a recombinant fragment of human ezrin corresponding to aa 362-585.

          Target Information

          The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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