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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • F4/80 Antibodies

          Proteintech

          F4/80 Polyclonal Antibody

          121 References
          View all (56) F4/80 antibodies

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          Cite F4/80 Polyclonal Antibody

          • Antibody Testing Data (6)
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          FIGURE: 1 / 6

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          F4/80 Antibody (28463-1-AP) in IHC (P)

          Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue slide using (Product # 28463-1-AP) F4/80 antibody at dilution of 1:2,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Flow Cytometry (Flow)

          Product Details

          28463-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 9 publications 9 publications

          Immunohistochemistry (IHC)

          -
          View 57 publications 57 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:2,000
          -

          Immunocytochemistry (ICC/IF)

          -
          View 46 publications 46 publications

          Flow Cytometry (Flow)

          Assay-dependent
          View 7 publications 7 publications

          Not applicable (N/A)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Adgre1 Fusion Protein Ag28252

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          367 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice

          Product Specific Information

          Aliquoting is unnecessary for -20°C storage.

          Target Information

          F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71

          View more View less

          Gene Aliases: Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3

          View more View less

          UniProt ID: (Mouse) Q61549

          View more View less

          Entrez Gene ID: (Mouse) 13733

          View more View less

          Function(s)
          signal transducer activity transmembrane signaling receptor activity G-protein coupled receptor activity calcium ion binding
          Process(es)
          signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway
          It has to be done as per old AB suggested Products section.
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