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          • Primary Antibodies ›
          • F4/80 Antibodies

          Invitrogen

          F4/80 Monoclonal Antibody (BM8), Functional Grade, eBioscience™

          98 Published Figures
          426 References
          View all (56) F4/80 antibodies

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          Datasheet
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          Cite F4/80 Monoclonal Antibody (BM8), Functional Grade, eBioscience™

          • Published Figures (98)
          F4/80 Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.
          F4/80 Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.

          FIGURE: 1 / 98

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          F4/80 Antibody (16-4801-86) in IHC

          Cellular & molecular biology letters 2023 - Fig. 3 CEBPB is associated with macrophage activation in LN. A Analysis of single-cell expression of CEBPB in the kidney using the Human Protein Atlas database. B Correlation between CEBPB expression and macrophage infiltration in kidney tissues ( n = 23) by Pearson's correlation analysis. C Immunohistochemical detection of CD68 in the kidney tissues of LN mice with CEBPB downregulation. D Immunohistochemic... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen), Immunohistochemistry (PFA fixed) (IHC (F), IHC (PFA))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)

          Product Details

          16-4801-86

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 174 publications 174 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 9 publications 9 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 3 publications 3 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 10 publications 10 publications

          Immunocytochemistry (ICC/IF)

          -
          View 40 publications 40 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 187 publications 187 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Functional Assay (Functional)

          Assay-Dependent
          -

          Control (Ctrl)

          Assay-Dependent
          -

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Hamster, Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          BM8

          Conjugate

          Functional Grade Functional Grade Functional Grade
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Alexa Fluor 561
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 563
          • Brilliant UV 661
          • Brilliant Violet 421
          • Brilliant Violet 650
          • eFluor 450
          • eFluor 506
          • eFluor 570
          • eFluor 660
          • FITC
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_469097

          Product Specific Information

          Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 125 kDa transmembrane protein. The F4/80 antigen is expressed by a majority of mature macrophages and is the best marker for this population of cells. However, other cell types such as Langerhans cells and liver Kupffer cells have been reported to express this antigen. Expression of F4/80 commences during early myeloid development and is upregulated on all BM cells stimulated in vitro with M-CSF. It has been shown that some cytokines downregulate the expression of F4/80 resulting in lack of F4/80 antigen on a subpopulation of macrophages, especially in the lymphoid microenvironment in vivo.

          Applications Reported: BM8 has been reported for use in flow cytometric analysis.

          Applications Tested: The BM8 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Storage and handling: Use in a sterile environment.

          Filtration: 0.2 µm post-manufacturing filtered.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

          Aggregation: Less than 10%, as determined by HPLC.

          Target Information

          F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71

          View more View less

          Gene Aliases: Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3

          View more View less

          UniProt ID: (Mouse) Q61549

          View more View less

          Entrez Gene ID: (Mouse) 13733

          View more View less

          Function(s)
          signal transducer activity transmembrane signaling receptor activity G-protein coupled receptor activity calcium ion binding
          Process(es)
          signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

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