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          • Primary Antibodies ›
          • F4/80 Antibodies

          Invitrogen

          F4/80 Monoclonal Antibody (BM8), Alexa Fluor™ 488, eBioscience™

          97 Published Figures
          408 References
          View all (56) F4/80 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite F4/80 Monoclonal Antibody (BM8), Alexa Fluor™ 488, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (97)
          F4/80 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          F4/80 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 98

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          F4/80 Antibody (53-4801-82) in Flow

          Staining of mouse resident peritoneal exudate cells with Anti-Mouse CD11b APC (Product # 17-0112-82) and 0.25 µg of Rat IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (left) or 0.25 µg of Anti-Mouse F4/80 Antigen Alexa Fluor® 488 (right). Cells in the large scatter population were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          F4/80 Antibody in Flow Cytometry (Flow)
          F4/80 Antibody in Flow Cytometry (Flow)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)

          Product Details

          53-4801-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 155 publications 155 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 4 publications 4 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 5 publications 5 publications

          Immunocytochemistry (ICC/IF)

          -
          View 46 publications 46 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 195 publications 195 publications

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Hamster, Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          BM8

          Conjugate

          Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
          • Unconjugated
          • Alexa Fluor 532 
          • Alexa Fluor 561
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 563
          • Brilliant UV 661
          • Brilliant Violet 421
          • Brilliant Violet 650
          • eFluor 450
          • eFluor 506
          • eFluor 570
          • eFluor 660
          • FITC
          • Functional Grade
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          499/520 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_469915

          Product Specific Information

          Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.

          Applications Reported: This BM8 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This BM8 antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71

          View more View less

          Gene Aliases: Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3

          View more View less

          UniProt ID: (Mouse) Q61549

          View more View less

          Entrez Gene ID: (Mouse) 13733

          View more View less

          Function(s)
          signal transducer activity transmembrane signaling receptor activity G-protein coupled receptor activity calcium ion binding
          Process(es)
          signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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