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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • F4/80 Antibodies

          Invitrogen

          F4/80 Monoclonal Antibody (CI:A3-1)

          Advanced Verification
          12 Published Figures
          51 References
          View all (56) F4/80 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite F4/80 Monoclonal Antibody (CI:A3-1)

          • Antibody Testing Data (2)
          • Published Figures (12)
          • Advanced Verification (1)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 15

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          F4/80 Antibody (MA1-91124) in ICC/IF

          Immunofluorescence analysis of F4/80 was performed using 70% confluent log phase RAW 264.7 and NMuMG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with F4/80 Monoclonal Antibody (CI:A3-1) (Product # MA1-91124) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006)... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry (ICC/IF)
          F4/80 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (IHC)
          F4/80 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          F4/80 Antibody

          Product Details

          MA1-91124

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          10 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 16 publications 16 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:100
          View 1 publication 1 publication

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          Assay-dependent
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          1:50-1:100
          View 17 publications 17 publications

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Radioimmune Assays (RIA)

          Assay-dependent
          -

          Immunomicroscopy (IM)

          Assay-dependent
          -

          Miscellaneous PubMed (MISC)

          -
          View 11 publications 11 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Rat / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          CI:A3-1

          Immunogen

          Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2277854

          Product Specific Information

          Positive control of mouse macrophages suggested. MA1-91124 detects a band of approximately 160 kDa due to the protein being heavily glycosylated, predicted molecular weight of precursor protein is 102 kDa.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          This antibody recognizes murine F4/80 antigen which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

          Target Information

          F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; egf-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71

          View more View less

          Gene Aliases: ADGRE1; DD7A5-7; EGF-TM7; EMR1; F4/80; Gpf480; Ly71; TM7LN3

          View more View less

          UniProt ID: (Human) Q14246, (Mouse) Q61549, (Rat) Q5Y4N8

          View more View less

          Entrez Gene ID: (Human) 2015, (Mouse) 13733, (Rat) 316137

          View more View less

          Function(s)
          G-protein coupled receptor activity calcium ion binding signal transducer activity transmembrane signaling receptor activity
          Process(es)
          G-protein coupled receptor signaling pathway adaptive immune response cell adhesion cell surface receptor signaling pathway signal transduction
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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