Invitrogen

FABP7 Polyclonal Antibody

FABP7 Antibody in Immunocytochemistry (ICC/IF)

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FABP7 Antibody (PA5-24949) in ICC/IF

Immunofluorescence analysis of Fatty acid-binding protein, brain was performed using 70% confluent log phase SK-MEL-5 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with FABP7 Polyclonal Antibody (Product # PA5-24949) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fl... View More

FABP7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FABP7 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

FABP7 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. SK-MEL-5 cells were transfected with Fatty acid-binding protein, brain siRNA and decrease in signal intensity was observed in Western Blot application using Anti-FABP7 Polyclonal Antibody (Product # PA5-24949). {KD}

Product Details

PA5-24949

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 104-132 amino acids from the C-terminal region of human FABP7

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2542449

Product Specific Information

This antibody is predicted to react with bovine and chicken based on sequence homology.

Target Information

FABP7 was initially isolated from a human fetal brain cDNA library and whose mRNA was expressed in adult brain and muscle tissues at low levels. The protein encoded by this gene is a member of the fatty acid binding protein (FABPs) family, a group of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism. FABP7 is a downstream gene of the Pax6 transcription factor and has been suggested to be essential for the maintenance of neuroepithelial cells during early cortical development. More recently, FABP7 was found to be frequently expressed in melanomas. Down-regulation of FABP7 through RNAi expression could reduce in vitro cell proliferation and Matrigel invasion, suggesting that FABP7 may be a potential target for the development of diagnostic and therapeutic tools.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Self-renewing macrophages in dorsal root ganglia contribute to promote nerve regeneration.

Proceedings of the National Academy of Sciences of the United States of America

Feng R,Muraleedharan Saraswathy V,Mokalled MH,Cavalli V

PA5-24949 was used in Immunohistochemistry to reveal the heterogeneity of dorsal root ganglia macrophages and their potential neuro- and glioprotective roles, which may inform future therapeutic approaches to treat nerve injury.

Tue Feb 14 00:00:00 UTC 2023

Defective branched-chain amino acid catabolism in dorsal root ganglia contributes to mechanical pain.

EMBO reports

Xie H,Li J,Lian N,Xie M,Wu M,Tang K,Kang Y,Lu P,Li T

PA5-24949 was used in Immunohistochemistry (Frozen) to demonstrate a causal role of defective BCAA catabolism in mechanical pain by enhancing metabolite-mediated epigenetic regulation.

Mon Nov 06 00:00:00 UTC 2023

Disrupted Association of Sensory Neurons With Enveloping Satellite Glial Cells in Fragile X Mouse Model.

Frontiers in molecular neuroscience

Avraham O,Deng PY,Maschi D,Klyachko VA,Cavalli V

PA5-24949 was used in Immunohistochemistry to show that major structural defects in sensory neuron-satellite glial cell (SGC) association in the dorsal root ganglia, manifested by aberrant covering of the neuron and gaps between SGCs and the neuron along their contact surface.

Fri Apr 05 00:00:00 UTC 2024

Disrupted Association of Sensory Neurons With Enveloping Satellite Glial Cells in Fragile X Mouse Model.

Frontiers in molecular neuroscience

Avraham O,Deng PY,Maschi D,Klyachko VA,Cavalli V

Fri Apr 05 00:00:00 UTC 2024

Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair.

eLife

Avraham O,Feng R,Ewan EE,Rustenhoven J,Zhao G,Cavalli V

PA5-24949 was used in Immunohistochemistry to provide a map of the distinct DRG microenvironment responses to peripheral and central injuries at the single-cell level and highlights that manipulating non-neuronal cells could lead to avenues to promote functional recovery after CNS injuries or disease.

Wed Sep 29 00:00:00 UTC 2021

FABP7 upregulation induces a neurotoxic phenotype in astrocytes.

Glia

Killoy KM,Harlan BA,Pehar M,Vargas MR

PA5-24949 was used in Western Blot, Immunohistochemistry (Paraffin) to investigate the expression of FABP7 in animal models of human superoxide dismutase 1 (hSOD1)-linked ALS.

Tue Dec 01 00:00:00 UTC 2020

FABP7 upregulation induces a neurotoxic phenotype in astrocytes.

Glia

Killoy KM,Harlan BA,Pehar M,Vargas MR

PA5-24949 was used in Western Blot, Immunohistochemistry (Paraffin) to investigate the expression of FABP7 in animal models of human superoxide dismutase 1 (hSOD1)-linked ALS.

Tue Dec 01 00:00:00 UTC 2020

FABP7 upregulation induces a neurotoxic phenotype in astrocytes.

Glia

Killoy KM,Harlan BA,Pehar M,Vargas MR

PA5-24949 was used in Western Blot, Immunohistochemistry (Paraffin) to investigate the expression of FABP7 in animal models of human superoxide dismutase 1 (hSOD1)-linked ALS.

Tue Dec 01 00:00:00 UTC 2020

Satellite glial cells promote regenerative growth in sensory neurons.

Nature communications

Avraham O,Deng PY,Jones S,Kuruvilla R,Semenkovich CF,Klyachko VA,Cavalli V

Published figure using FABP7 polyclonal antibody (Product # PA5-24949) in Immunohistochemistry

Tue Sep 29 00:00:00 UTC 2020

Satellite glial cells promote regenerative growth in sensory neurons.

Nature communications

Avraham O,Deng PY,Jones S,Kuruvilla R,Semenkovich CF,Klyachko VA,Cavalli V

Published figure using FABP7 polyclonal antibody (Product # PA5-24949) in Immunohistochemistry

Tue Sep 29 00:00:00 UTC 2020

Satellite glial cells promote regenerative growth in sensory neurons.

Nature communications

Avraham O,Deng PY,Jones S,Kuruvilla R,Semenkovich CF,Klyachko VA,Cavalli V

Published figure using FABP7 polyclonal antibody (Product # PA5-24949) in Immunohistochemistry

Tue Sep 29 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: B FABP; B-FABP; BLBP; brain lipid binding protein; Brain lipid-binding protein; Brain-type fatty acid-binding protein; DKFZp547J2313; FABP 7; Fatty acid-binding protein 7; Fatty acid-binding protein, brain; hypothetical protein DKFZp547J2313; Mammary-derived growth inhibitor related; mammary-derived growth inhibitor-related; OTTHUMP00000017119; RP11-548E19.1

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Gene Aliases: B-FABP; BFABP; BLBP; FABP7; FABPB; MRG

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UniProt ID: (Human) O15540, (Mouse) P51880

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Entrez Gene ID: (Human) 2173, (Mouse) 12140

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Function(s)

transporter activity lipid binding fatty acid binding

Process(es)

transport nervous system development negative regulation of cell proliferation triglyceride catabolic process cell proliferation in forebrain neurogenesis epithelial cell proliferation prepulse inhibition startle response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FABP7 Polyclonal Antibody

FABP7 Antibody (PA5-24949) in ICC/IF

Product Details

PA5-24949

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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