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Invitrogen
FAM105B Polyclonal Antibody
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FIGURE: 1 / 2
FAM105B Antibody (PA5-143981) in Flow
Flow cytometry analysis of FAM105B in 293T cells using FAM105B Polyclonal Antibody (Product # PA5-143981), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control a... View More
Product Details
PA5-143981
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human OTULIN recombinant protein (Position: A49-L352).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075195
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HELA whole cell, human THP-1 whole cell, human K562 whole cell, rat liver tissue, mouse liver tissue. IHC: human appendicitis tissue, human bladder cancer tissue, human tonsil tissue, human placenta tissue. ICC/IF: SIHA cell. Flow: 293T cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Otulin is a deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme otulin; family with sequence similarity 105, member B; gumby; OTU deubiquitinase with linear linkage specificity; OTU domain-containing deubiquitinase with linear linkage specificity; protein FAM105B; Ubiquitin thioesterase Gumby; Ubiquitin thioesterase otulin
Gene Aliases: C79097; FAM105B; GUM; m3Sapc; m7-1Sapc; OTULIN
UniProt ID: (Human) Q96BN8, (Mouse) Q3UCV8
Entrez Gene ID: (Human) 90268, (Mouse) 432940, (Rat) 100362554
angiogenesis
sprouting angiogenesis
immune system process
proteolysis
Wnt signaling pathway
negative regulation of NF-kappaB transcription factor activity
innate immune response
negative regulation of inflammatory response
canonical Wnt signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
protein linear deubiquitination
regulation of tumor necrosis factor-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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