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Product Details
H00253725-B01P
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
FAM21C (AAH06456, 1 a.a. approximately 283 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Product may be used with Western Blot (Transfected lysate).
The Isotype of this product is composed of an IgG Mixture.
Immunogen sequence: MSVPRGPIAQ WADGAISPNG HRPQLRAASG EDSTEEALAA AAAPWEGGPV PGVDTSPFAK SLGHSRGEAD LFDSGDIFST GTGSQSVERT KPKAKIAENP ANPPVGGKAK SPMFPALGEA SSDDDLFQSA KPKPAKKTNP FPLLEDEDDL FTDQKVKKNE TKSSSQQDVI LTTQDIFEDD IFATEAIKPS QKTREKEKTL ESNLFDDNID IFADLTVKPK EKSKKKVEAK SIFDDDMDDI FSTGIQAKTT KPKSRSAQAA PEPRFEHKVS NIFDDPLNAF GGQ
Target Information
Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission (PubMed:19922874, PubMed:20498093, PubMed:22513087, PubMed:23331060). Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552). Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35 (PubMed:24980502). Required for the endosomal recruitment of CCC and retriever complexes subunits COMMD1 and CCDC93 as well as the retrievere complex subunit VPS35L (PubMed:25355947, PubMed:28892079). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: family with sequence similarity 21, member C; Vaccinia virus penetration factor; VPEF; WASH complex subunit 2C
Gene Aliases: FAM21A; FAM21C; KIAA0592; VPEF; WASHC2C
UniProt ID: (Human) Q9Y4E1
Entrez Gene ID: (Human) 253725
protein binding
phosphatidylinositol-4,5-bisphosphate binding
phosphatidylinositol-3,4,5-trisphosphate binding
phosphatidylinositol-5-phosphate binding
phosphatidylinositol-3-phosphate binding
phosphatidylinositol-3,4-bisphosphate binding
phosphatidylinositol-4-phosphate binding
phosphatidylinositol-3,5-bisphosphate binding
Performance Guarantee
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