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Rockland
FANCF Polyclonal Antibody
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FIGURE: 1 / 1
FANCF Antibody (600-401-674) in WB
Western blot using Rocklands affinity purified anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kDa in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irr... View More
Product Details
600-401-674
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chimpanzee,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal amino acid sequence of human FANCF.
Conjugate
Form
Liquid
Concentration
1.19 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human FANCF protein. A BLAST analysis was used to suggest cross-reactivity with FANCF protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
The Fanconi anemia complementation group currently includes FANCA, FANCB, FANCC, FANCD1 , FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ , FANCL, FANCM and FANCN . The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group F.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fanconi anemia group F protein; Fanconi anemia, complementation group F; MGC126856; Protein FACF
Gene Aliases: FAF; FANCF
UniProt ID: (Human) Q9NPI8
Entrez Gene ID: (Human) 2188
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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