Proteintech

FARSA Polyclonal Antibody

View all (10) FARSA antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (13)

FARSA Antibody in Immunocytochemistry (ICC/IF)

FARSA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

FARSA Antibody (18121-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using FARSA antibody 18121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

FARSA Antibody in Immunocytochemistry (ICC/IF)

FARSA Antibody in Immunocytochemistry (ICC/IF)

FARSA Antibody in Immunocytochemistry (ICC/IF)

FARSA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FARSA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FARSA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FARSA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FARSA Antibody in Western Blot (WB)

FARSA Antibody in Western Blot (WB)

FARSA Antibody in Western Blot (WB)

FARSA Antibody in Western Blot (WB)

FARSA Antibody in Western Blot (WB)

FARSA Antibody in Immunoprecipitation (IP)

Product Image Gallery

FARSA Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using FARSA antibody 18121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

FARSA Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using FARSA antibody 18121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

FARSA Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using FARSA antibody 18121-1-AP at 1:25 dilution and FITC- labeled Donkey anti-Rabbit IgG (green).

FARSA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue slide using (Product # 18121-1-AP) FARSA antibody at dilution of 1:1,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FARSA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue slide using (Product # 18121-1-AP) FARSA antibody at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FARSA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 40x lens).

FARSA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 10x lens).

FARSA Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 18121-1-AP) FARSA antibody at dilution of 1:8,000 incubated at room temperature for 1.5 hours.

FARSA Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

FARSA Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

FARSA Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FARSA Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FARSA Antibody in Immunoprecipitation (IP) — IP result of anti-FARSA (IP:18121-1-AP, 4ug; Detection:18121-1-AP 1:2000) with MCF-7 cells lysate 3240 ug.

Product Details

18121-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FARSA Fusion Protein Ag12647 (1-300 aa encoded by BC006495)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MADGQVAEL LLRRLEASDG GLDSAELAAE LGMEHQAVVG AVKSLQALGE VIEAELRSTK HWELTAEGEE IAREGSHEAR VFRSIPPEGL AQSELMRLPS GKVGFSKAMS NKWIRVDKSA ADGPRVFRVV DSMEDEVQRR LQLVRGGQAE KLGEKERSEL RKRKLLAEVT LKTYWVSKGS AFSTSISKQE TELSPEMISS GSWRDRPFKP YNFLAHGVLP DSGHLHPLLK VRSQFRQIFL EMGFTEMPTD NFIESSFWNF DALFQPQQHP ARDQHDTFFL RDPAEALQLP MDYVQRVKRT H (1-300 aa encoded by BC006495)

Target Information

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS2 is a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS1 encodes a mitochondrially-located phenylalanine-tRNA synthetase (PheRS) which consists of a single polypeptide chain unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and the eukaryotic cytoplasm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.

Nature communications

Zhou Q,Sun WW,Chen JC,Zhang HL,Liu J,Lin Y,Lin PC,Wu BX,An YP,Huang L,Sun WX,Zhou XW,Li YM,Yuan YY,Zhao JY,Xu W,Zhao SM

Mon Jul 25 00:00:00 UTC 2022

Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.

Nature communications

Zhou Q,Sun WW,Chen JC,Zhang HL,Liu J,Lin Y,Lin PC,Wu BX,An YP,Huang L,Sun WX,Zhou XW,Li YM,Yuan YY,Zhao JY,Xu W,Zhao SM

Mon Jul 25 00:00:00 UTC 2022

Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.

Nature communications

Zhou Q,Sun WW,Chen JC,Zhang HL,Liu J,Lin Y,Lin PC,Wu BX,An YP,Huang L,Sun WX,Zhou XW,Li YM,Yuan YY,Zhao JY,Xu W,Zhao SM

Mon Jul 25 00:00:00 UTC 2022

Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.

Nature communications

Zhou Q,Sun WW,Chen JC,Zhang HL,Liu J,Lin Y,Lin PC,Wu BX,An YP,Huang L,Sun WX,Zhou XW,Li YM,Yuan YY,Zhao JY,Xu W,Zhao SM

Mon Jul 25 00:00:00 UTC 2022

Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.

Nature communications

Zhou Q,Sun WW,Chen JC,Zhang HL,Liu J,Lin Y,Lin PC,Wu BX,An YP,Huang L,Sun WX,Zhou XW,Li YM,Yuan YY,Zhao JY,Xu W,Zhao SM

Mon Jul 25 00:00:00 UTC 2022

Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.

Nature communications

Zhou Q,Sun WW,Chen JC,Zhang HL,Liu J,Lin Y,Lin PC,Wu BX,An YP,Huang L,Sun WX,Zhou XW,Li YM,Yuan YY,Zhao JY,Xu W,Zhao SM

Mon Jul 25 00:00:00 UTC 2022

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Nature communications

Jhanji M,Rao CN,Massey JC,Hope MC,Zhou X,Keene CD,Ma T,Wyatt MD,Stewart JA,Sajish M

Fri Jun 10 00:00:00 UTC 2022

Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase.

Autoimmunity reviews

Muro Y,Yamashita Y,Koizumi H,Ogawa-Momohara M,Takeichi T,Mitsuma T,Akiyama M

Thu Dec 01 00:00:00 UTC 2022

Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase.

Autoimmunity reviews

Muro Y,Yamashita Y,Koizumi H,Ogawa-Momohara M,Takeichi T,Mitsuma T,Akiyama M

Thu Dec 01 00:00:00 UTC 2022

FARS1-related disorders caused by bi-allelic mutations in cytosolic phenylalanyl-tRNA synthetase genes: Look beyond the lungs!

Clinical genetics

Schuch LA,Forstner M,Rapp CK,Li Y,Smith DEC,Mendes MI,Delhommel F,Sattler M,Emiralioğlu N,Taskiran EZ,Orhan D,Kiper N,Rohlfs M,Jeske T,Hastreiter M,Gerstlauer M,Torrent-Vernetta A,Moreno-Galdó A,Kammer B,Brasch F,Reu-Hofer S,Griese M

Tue Jun 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: CML33; phenylalanine tRNA ligase 1, alpha, cytoplasmic; phenylalanine--tRNA ligase alpha chain; Phenylalanine--tRNA ligase alpha subunit; phenylalanine-tRNA synthetase alpha-subunit; phenylalanine-tRNA synthetase-like, alpha subunit; phenylalanyl-tRNA synthetase alpha chain; Phenylalanyl-tRNA synthetase alpha subunit; phenylalanyl-tRNA synthetase, alpha subunit; phenylalanyl-tRNA synthetase-like, alpha subunit; PheRS

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Gene Aliases: 0610012A19Rik; CML33; FARS; FARSA; FARSL; FARSLA; FRSA; PheHA

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UniProt ID: (Human) Q9Y285, (Rat) Q505J8, (Mouse) Q8C0C7

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Entrez Gene ID: (Human) 2193, (Rat) 288917, (Mouse) 66590

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tRNA binding phenylalanine-tRNA ligase activity protein binding ATP binding poly(A) RNA binding nucleotide binding aminoacyl-tRNA ligase activity ligase activity

tRNA aminoacylation for protein translation phenylalanyl-tRNA aminoacylation protein heterotetramerization translation biological_process tRNA aminoacylation

Performance Guarantee

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