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Invitrogen
FARSA Polyclonal Antibody
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FIGURE: 1 / 1
FARSA Antibody (PA5-90609) in WB
Western blot analysis of FARSA in extracts of various cell lines. Samples were incubated with FARSA Polyclonal antibody (Product # PA5-90609) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-90609
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 210-486 of human FARSA (NP_0044521)
Conjugate
Form
Liquid
Concentration
1.36 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806143
Product Specific Information
Immunogen sequence: YNFLAHGVLP DSGHLHPLLK VRSQFRQIFL EMGFTEMPTD NFIESSFWNF DALFQPQQHP ARDQHDTFFL RDPAEALQLP MDYVQRVKRT HSQGGYGSQG YKYNWKLDEA RKNLLRTHTT SASARALYRL AQKKPFTPVK YFSIDRVFRN ETLDATHLAE FHQIEGVVAD HGLTLGHLMG VLREFFTKLG ITQLRFKPAY NPYTEPSMEV FSYHQGLKKW VEVGNSGVFR PEMLLPMGLP ENVSVIAWGL SLERPTMIKY GINNIRELVG HKVNLQM; Positive Samples: HeLa, Mouse liver; Cellular Location: Cytoplasm
Target Information
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS2 is a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS1 encodes a mitochondrially-located phenylalanine-tRNA synthetase (PheRS) which consists of a single polypeptide chain unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and the eukaryotic cytoplasm.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CML33; phenylalanine tRNA ligase 1, alpha, cytoplasmic; phenylalanine--tRNA ligase alpha chain; Phenylalanine--tRNA ligase alpha subunit; phenylalanine-tRNA synthetase alpha-subunit; phenylalanine-tRNA synthetase-like, alpha subunit; phenylalanyl-tRNA synthetase alpha chain; Phenylalanyl-tRNA synthetase alpha subunit; phenylalanyl-tRNA synthetase, alpha subunit; phenylalanyl-tRNA synthetase-like, alpha subunit; PheRS
Gene Aliases: 0610012A19Rik; CML33; FARS; FARSA; FARSL; FARSLA; FRSA; PheHA
UniProt ID: (Human) Q9Y285, (Mouse) Q8C0C7
Entrez Gene ID: (Human) 2193, (Mouse) 66590
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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